Freezing and Thawing – Cell Culture

TYPE

Cell Culture & Laboratory Techniques

LEVEL

University, Apprenticeship, College, Post-Graduate

EQUIPMENT

General Laboratory Equipment & Specialist Equipment

The “Freezing and Thawing” collection forms part of a wider suite of virtual cell culture training resources available for download on our website. While introductory modules focus on developing essential skills such as sterile working practices and accurate pipetting, it is equally important that new users gain confidence in carrying out common cell culture procedures—one of the most frequent being the freezing of cells in cryogenic vials.

This collection also introduces more advanced techniques, including the use of specialist equipment such as plasmatherms for thawing bags. By practicing these methods virtually, learners become familiar not only with the core processes but also with variations they may encounter in different laboratory environments.

Core Cell Culture & Lab Practice Training

In addition to technique, the modules highlight the importance of effective laboratory organisation—such as arranging reagents, maintaining appropriate storage conditions, and preventing waste or degradation of valuable biological materials. These practices, though critical, are often underemphasised in formal training programmes. In many cases, personnel are expected to follow standard operating procedures (SOPs) with minimal, or sometimes no, hands-on training beforehand.

By addressing these overlooked areas, the “Freezing and Thawing” protocols aim to build both technical skill and user confidence which in turn ensures the integrity of valuable specimens reagents.

We recommend completing this collection after the Pipetting and Fundamental Laboratory Skills modules, as it builds naturally on those foundations. Operators can then expand their training further by incorporating additional SOPs and cell culture practices relevant to their specific laboratory.

SOP List

Freezing and thawing bags and bottles.
Fridge andfreezer storage.
Freezing cells in cryovials.

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Learning Objectives

Practice assessing different cell culture reagents storage requirements.
Understand how to properly organise a fridge and freezer for storage.
Learn the correct procedure for freezing cells in cryovials.
Learn how to use the plasma thaw device.

Freezing and Thawing – Cell Culture

Core Cell Culture & Lab Practice Training

Learning Objectives

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