{ "version": "v3", "compatibility": "0.1.4", "collections": [ { "name": "Freezing and Thawing", "sops": [ { "name": "Freezing and Thawing bags and bottles", "steps": [ "Objective: Practice freezing and thawing cryobags and bottles.", "Turn on Microbiological safety cabinet (MSC) by pressing the power button on the MSC’s touch display. ", "On the MSC’s touch display, turn on MSC light.", "On the MSC’s touch display, turn on laminar airflow (fan).", "On the MSC’s touch display, use the upwards arrow button to lift MSC glass sash. Wait until the sash is fully open before continuing.", "Grab the ethanol spray bottle located on the table opposite the MSC and liberally spray both hands to sanitise them before working in the MSC.", "Replace the FBS bottle lid with a fluid transfer cap.", "Hang the 250ml cryobag and using the pre-attached tubing, connect it up to the bottle. ", "Insert the midpoint of the tubing into the pump head and open the pinch clamps on the tubing line. Pump ~200ml FBS into the bag and then stop pumping.", "Remove the fluid transfer cap from the FBS bottle and onto the empty bottle, with the tubing attached.", "Start the pump and run until the additional FBS drains from the tubing.", "Stop the pump and remove the tubing end from the fluid transfer cap. ", "Re-attach the cap to the end of the tubing and close the pinch clamp. ", "Re-cap the bottle of FBS and dispose of the fluid transfer cap. ", "Place both the cryobag and bottle of FBS inside the -80 freezer and allow to freeze.", "To rapidly thaw the bottle of FBS, you can use the waterbath.", "Open the waterbath lid and select the temperature of the water on the device touchscreen. ", "Place the bottle of FBS inside the waterbath and close the lid. You can take the bottle out after defrosting the cryobag.", "However, to defrost the bag you should use the Plasmatherm.", "Press the power icon on the plasmatherm.", "press the blue button to open the Plasmatherm. ", "Place the frozen cryobag inside the plasmatherm.", "Close the plasmatherm lid using the blue button.", "Tap the icon for the menu and navigate to program and then OK. ", "Navigate to start USER and then press OK.", "Once completed, open the plasmatherm and remove the thawed bag of FBS. ", "You now have a thawed bottle and bag of FBS." ], "layout": { "id": "main", "name": "Main grid", "x": 0, "y": 0, "w": 40, "h": 40, "ueBlueprint": "", "relativeRotation": 0, "absoluteRotation": 0, "isSurface": true, "requiresSurface": false, "items": [ { "id": "MSC_Small_0", "name": "MSC Small", "x": 26, "y": 0, "w": 13, "h": 28, "ueBlueprint": "/Game/Instruments/MSC/BP_MSC_Herasafe_Standing.BP_MSC_Herasafe_Standing_C", "absoluteRotation": 90, "relativeRotation": 90, "gridImage": "devices/msc.png", "thumbnailImage": "devices/msc.png", "isSurface": true, "requiresSurface": false, "items": [ { "id": "Peristaltic_Pump_Digital_0", "name": "Peristaltic Pump Digital", "x": 3, "y": 15, "w": 9, "h": 5, "ueBlueprint": "/Game/Instruments/LiquidPumps/PeristalticPump/BP_Peristaltic_Pump.BP_Peristaltic_Pump_C", "absoluteRotation": 90, "relativeRotation": 0, "gridImage": "devices/peristaltic_pump.png", "thumbnailImage": "devices/peristaltic_pump.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Fluid_Transfer_Cap_0", "name": "Fluid Transfer Cap", "x": 8, "y": 9, "w": 4, "h": 5, "ueBlueprint": "/Game/Instruments/Tubing/Dispensers/BP_Dispenser_Connector_Fluid_Transfer_Cap.BP_Dispenser_Connector_Fluid_Transfer_Cap_C", "absoluteRotation": 90, "relativeRotation": 0, "gridImage": "tubing/dispenser_fluid_transfer_cap.png", "thumbnailImage": "tubing/fluid_transfer_cap.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Fetal_Bovine_Serum_(FBS)_1L_0", "name": "Fetal Bovine Serum (FBS) 1L", "x": 6, "y": 22, "w": 3, "h": 3, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_FBS_1L.BP_Bottle_FBS_1L_C", "absoluteRotation": 90, "relativeRotation": 0, "gridImage": "containers/bottle02_1l.png", "thumbnailImage": "containers/bottle1l_fbs.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Bottle_250ml_0", "name": "Bottle 250ml", "x": 3, "y": 24, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/GL45/BP_Bottle_GL45_250ml.BP_Bottle_GL45_250ml_C", "absoluteRotation": 90, "relativeRotation": 0, "gridImage": "containers/bottle02_250ml.png", "thumbnailImage": "containers/bottle02_250ml.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Cryobag_250ml_0", "name": "Cryobag 250ml", "x": 2, "y": 3, "w": 5, "h": 3, "ueBlueprint": "/Game/Instruments/Containers/Bags/BP_Fluid_Bag_BioFluid_250ml.BP_Fluid_Bag_BioFluid_250ml_C", "absoluteRotation": 90, "relativeRotation": 0, "gridImage": "bags/cryobag_250ml.png", "thumbnailImage": "bags/cryobag.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Bag_Stand_50cm_0", "name": "Bag Stand 50cm", "x": 8, "y": 3, "w": 4, "h": 4, "ueBlueprint": "/Game/Instruments/BagStands/BP_Bag_Stand_50cm.BP_Bag_Stand_50cm_C", "absoluteRotation": 90, "relativeRotation": 0, "gridImage": "bags/bag_stand_50cm.png", "thumbnailImage": "bags/bag_stand_50cm.png", "isSurface": false, "requiresSurface": true, "items": [] } ] }, { "id": "Table_180_x_60_0", "name": "Table 180 x 60", "x": 4, "y": 28, "w": 36, "h": 12, "ueBlueprint": "/Game/Instruments/Tables/BP_Table_180x60.BP_Table_180x60_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "furniture/table_180x60.png", "thumbnailImage": "furniture/table_large.png", "isSurface": true, "requiresSurface": true, "items": [ { "id": "Plasma_Thaw_Device_0", "name": "Plasma Thaw Device", "x": 13, "y": 0, "w": 7, "h": 12, "ueBlueprint": "/Game/Instruments/Plasmatherm/BP_Plasmatherm_CG.BP_Plasmatherm_CG_C", "absoluteRotation": 180, "relativeRotation": 180, "gridImage": "devices/thaw_device_plasmatherm.png", "thumbnailImage": "devices/thaw_device_plasmatherm.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Water_Bath_0", "name": "Water Bath", "x": 0, "y": 3, "w": 11, "h": 7, "ueBlueprint": "/Game/Instruments/WaterBath/BP_Water_Bath.BP_Water_Bath_C", "absoluteRotation": 180, "relativeRotation": 180, "gridImage": "devices/water_bath.png", "thumbnailImage": "devices/water_bath.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Ethanol_Spray_Bottle_0", "name": "Ethanol Spray Bottle", "x": 21, "y": 1, "w": 2, "h": 3, "ueBlueprint": "/Game/Instruments/Sanitising/BP_Spray.BP_Spray_C", "absoluteRotation": 90, "relativeRotation": 90, "gridImage": "cleaning/spray_bottle.png", "thumbnailImage": "cleaning/spray_bottle.png", "isSurface": false, "requiresSurface": true, "items": [] } ] }, { "id": "Bio_Bin_6L_0", "name": "Bio Bin 6L", "x": 15, "y": 1, "w": 5, "h": 4, "ueBlueprint": "/Game/Instruments/Bins/BP_Bin_6L_Bio.BP_Bin_6L_Bio_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "cleaning/bio_bin_6l.png", "thumbnailImage": "cleaning/bio_bin_6l.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Freezer_-80_0", "name": "Freezer -80", "x": 0, "y": 0, "w": 14, "h": 17, "ueBlueprint": "/Game/Instruments/Incubators/BP_Freezer.BP_Freezer_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/fridge_freezer_tsx.png", "thumbnailImage": "devices/fridge_freezer_tsx.png", "isSurface": false, "requiresSurface": true, "items": [] } ], "thumbnailImage": "", "gridImage": "", "parts": [] } }, { "version": "v2", "name": "Fridge and Freezer storage ", "steps": [ "Purpose and Scope: This SOP allows users to learn where and how to store different cell culture chemicals and reagents between lab work. ", "There are some key considerations for storing in laboratories:", "1. Segregate by compatibility (flammables, oxidisers, acids, bases, biological vs chemical). Do not store flammable solvents in a domestic/non-explosion-proof fridge.", "2. Keep clear labelling (see example below) and an up-to-date inventory.", "3. Use continuous temp monitoring with alarms when possible; otherwise record temps daily.", "On receipt of the reagents, check the package integrity and delivery temperature and read the SDS / manufacturer storage instructions immediately for storage requirements. For these reagents the storage temp can be found on the side of the bottle, however some reagents may also be suitable for aliquoting and freezing in the -80. ", "Aliquoting upon receipt is preferable as it prevents repeated freeze thaw cycles. ", "Place liquids in secondary containment (tray or sealed box) if necessary. ", "Segregate items by hazard class and stability (e.g., flammables separate; oxidisers away from organics; acids separate from cyanides). Follow SDS compatibility guidance.", "Put reagents away from the door (middle shelves/back) where temperature is most stable. Avoid door shelves for long-term storage.", "Do not block vents; allow airflow between items.", "Group similar items together and clearly label shelves or zones.", "Do not overload shelves or exceed manufacturer load limits.", "Keep an accessible inventory (electronic or log) with location and expiry dates; review monthly or quarterly.", "Considering this, you should now try your hand at organising reagents and chemicals into the freezers and fridges. " ], "layout": { "id": "main", "name": "Main grid", "x": 0, "y": 0, "w": 40, "h": 40, "ueBlueprint": "", "relativeRotation": 0, "absoluteRotation": 0, "isSurface": true, "requiresSurface": false, "items": [ { "id": "Freezer_-80_0", "name": "Freezer -80", "x": 0, "y": 0, "w": 14, "h": 17, "ueBlueprint": "/Game/Instruments/Incubators/BP_Freezer.BP_Freezer_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/fridge_freezer_tsx.png", "thumbnailImage": "devices/fridge_freezer_tsx.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Table_120_x_45_0", "name": "Table 120 x 45", "x": 16, "y": 31, "w": 24, "h": 9, "ueBlueprint": "/Game/Instruments/Tables/BP_Table_Stand_Small_Thin.BP_Table_Stand_Small_Thin_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "furniture/table_thin.png", "thumbnailImage": "furniture/table_thin.png", "isSurface": true, "requiresSurface": true, "items": [ { "id": "DMEM_1L_0", "name": "DMEM 1L", "x": 20, "y": 5, "w": 3, "h": 3, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_DMEM_1L.BP_Bottle_DMEM_1L_C", "absoluteRotation": 180, "relativeRotation": 180, "gridImage": "containers/bottle02_1l.png", "thumbnailImage": "containers/bottle1l_dmem.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Fetal_Bovine_Serum_(FBS)_1L_1", "name": "Fetal Bovine Serum (FBS) 1L", "x": 16, "y": 5, "w": 3, "h": 3, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_FBS_1L.BP_Bottle_FBS_1L_C", "absoluteRotation": 180, "relativeRotation": 180, "gridImage": "containers/bottle02_1l.png", "thumbnailImage": "containers/bottle1l_fbs.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Fetal_Bovine_Serum_(FBS)_1L_2", "name": "Fetal Bovine Serum (FBS) 1L", "x": 20, "y": 1, "w": 3, "h": 3, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_FBS_1L.BP_Bottle_FBS_1L_C", "absoluteRotation": 180, "relativeRotation": 180, "gridImage": "containers/bottle02_1l.png", "thumbnailImage": "containers/bottle1l_fbs.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Fetal_Bovine_Serum_(FBS)_1L_0", "name": "Fetal Bovine Serum (FBS) 1L", "x": 16, "y": 1, "w": 3, "h": 3, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_FBS_1L.BP_Bottle_FBS_1L_C", "absoluteRotation": 180, "relativeRotation": 180, "gridImage": "containers/bottle02_1l.png", "thumbnailImage": "containers/bottle1l_fbs.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "General_Culture_Media_0", "name": "General Culture Media", "x": 11, "y": 5, "w": 3, "h": 3, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Cell_Culture_Medium_1L.BP_Bottle_Cell_Culture_Medium_1L_C", "absoluteRotation": 180, "relativeRotation": 180, "gridImage": "containers/bottle02_1l.png", "thumbnailImage": "containers/general_culture_media1L.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "DMEM_1L_1", "name": "DMEM 1L", "x": 7, "y": 5, "w": 3, "h": 3, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_DMEM_1L.BP_Bottle_DMEM_1L_C", "absoluteRotation": 180, "relativeRotation": 180, "gridImage": "containers/bottle02_1l.png", "thumbnailImage": "containers/bottle1l_dmem.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Sterile_PBS_1L_0", "name": "Sterile PBS 1L", "x": 3, "y": 5, "w": 3, "h": 3, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_PBS_1L.BP_Bottle_PBS_1L_C", "absoluteRotation": 180, "relativeRotation": 180, "gridImage": "containers/bottle01_1l.png", "thumbnailImage": "containers/bottle1l_sterile_pbs.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Vial_of_Cells_0", "name": "Vial of Cells", "x": 11, "y": 1, "w": 4, "h": 3, "ueBlueprint": "/Game/Instruments/Containers/Dispensers/BP_Dispenser_Vial_Of_Cells.BP_Dispenser_Vial_Of_Cells_C", "absoluteRotation": 180, "relativeRotation": 180, "gridImage": "containers/dispenser_vial_of_cells.png", "thumbnailImage": "containers/vial_of_cells.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Bovine_Serum_Albumin_100ml_0", "name": "Bovine Serum Albumin 100ml", "x": 8, "y": 3, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Bovine_Serum_Albumin_500ml.BP_Bottle_Bovine_Serum_Albumin_500ml_C", "absoluteRotation": 180, "relativeRotation": 180, "gridImage": "containers/bottle02_100ml.png", "thumbnailImage": "containers/bottle100ml_bovine_serum_albumin.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Bovine_Serum_Albumin_100ml_1", "name": "Bovine Serum Albumin 100ml", "x": 6, "y": 3, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Bovine_Serum_Albumin_500ml.BP_Bottle_Bovine_Serum_Albumin_500ml_C", "absoluteRotation": 180, "relativeRotation": 180, "gridImage": "containers/bottle02_100ml.png", "thumbnailImage": "containers/bottle100ml_bovine_serum_albumin.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Paraformaldehyde_Fixation_Buffer_100ml_0", "name": "Paraformaldehyde Fixation Buffer 100ml", "x": 4, "y": 3, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_PFA_100ml.BP_Bottle_PFA_100ml_C", "absoluteRotation": 180, "relativeRotation": 180, "gridImage": "containers/bottle02_100ml.png", "thumbnailImage": "containers/bottle100ml_paraformaldehyde_fixation_buffer.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "DMSO_100ml_0", "name": "DMSO 100ml", "x": 2, "y": 3, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_DMSO_100ml.BP_Bottle_DMSO_100ml_C", "absoluteRotation": 180, "relativeRotation": 180, "gridImage": "containers/bottle02_100ml.png", "thumbnailImage": "containers/bottle100ml_dmso.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Accutase_100ml_0", "name": "Accutase 100ml", "x": 2, "y": 1, "w": 1, "h": 1, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Accutase_100ml.BP_Bottle_Accutase_100ml_C", "absoluteRotation": 180, "relativeRotation": 180, "gridImage": "containers/bottle03_100ml.png", "thumbnailImage": "containers/bottle100ml_accutase.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Glutamine_100ml_0", "name": "Glutamine 100ml", "x": 4, "y": 1, "w": 1, "h": 1, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Glutamine_100ml.BP_Bottle_Glutamine_100ml_C", "absoluteRotation": 180, "relativeRotation": 180, "gridImage": "containers/bottle03_100ml.png", "thumbnailImage": "containers/bottle100ml_glutamine.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Flow_Buffer_100ml_0", "name": "Flow Buffer 100ml", "x": 7, "y": 1, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Flow_Buffer_100ml.BP_Bottle_Flow_Buffer_100ml_C", "absoluteRotation": 180, "relativeRotation": 180, "gridImage": "containers/bottle02_100ml.png", "thumbnailImage": "containers/bottle100ml_flow_buffer.png", "isSurface": false, "requiresSurface": true, "items": [] } ] }, { "id": "Freezer_-20_0", "name": "Freezer -20", "x": 14, "y": 1, "w": 13, "h": 14, "ueBlueprint": "/Game/Instruments/Incubators/BP_Small_Fridge_Freezer.BP_Small_Fridge_Freezer_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/fridge_freezer_small.png", "thumbnailImage": "devices/fridge_freezer_small.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Fridge_0", "name": "Fridge", "x": 27, "y": 1, "w": 13, "h": 14, "ueBlueprint": "/Game/Instruments/Incubators/BP_Small_Fridge_Freezer.BP_Small_Fridge_Freezer_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/fridge_freezer_small.png", "thumbnailImage": "devices/fridge_freezer_small.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Table_60_x_45_0", "name": "Table 60 x 45", "x": 4, "y": 31, "w": 12, "h": 9, "ueBlueprint": "/Game/Instruments/Tables/BP_Table_Stand_Small.BP_Table_Stand_Small_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "furniture/table_stand.png", "thumbnailImage": "furniture/table_stand.png", "isSurface": true, "requiresSurface": true, "items": [ { "id": "Corrosives_Cabinet_0", "name": "Corrosives Cabinet", "x": 1, "y": 2, "w": 8, "h": 6, "ueBlueprint": "/Game/Instruments/HazardsCabinets/BP_Hazards_Cabinet_Corrosive.BP_Hazards_Cabinet_Corrosive_C", "absoluteRotation": 180, "relativeRotation": 180, "gridImage": "furniture/cabinet_small.png", "thumbnailImage": "furniture/cabinet_corrosives.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Nitric_Acid_1L_0", "name": "Nitric Acid 1L", "x": 10, "y": 1, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Nitric_Acid_1L.BP_Bottle_Nitric_Acid_1L_C", "absoluteRotation": 180, "relativeRotation": 180, "gridImage": "containers/bottle06_1l.png", "thumbnailImage": "containers/bottle1l_nitric_acid.png", "isSurface": false, "requiresSurface": true, "items": [] } ] } ], "thumbnailImage": "", "gridImage": "", "parts": [] } }, { "version": "v2", "name": "Freezing cells in vials", "steps": [ "Objective: This protocol teaches the steps to Freeze cells into vials. ", "Turn on Microbiological safety cabinet (MSC) by pressing the power button on the MSC’s touch display. ", "On the MSC’s touch display, turn on MSC light.", "On the MSC’s touch display, turn on laminar airflow (fan).", "On the MSC’s touch display, use the upwards arrow button to lift MSC glass sash. Wait until the sash is fully open before continuing.", "Grab the ethanol spray bottle located on the table opposite the MSC and liberally spray both hands to sanitise them before working in the MSC.", "Retrieve enough vials to fill the cool cell container and place them in a rack. ", "Retrieve a tube of cell suspension (in FBS) from the dispenser.", "Using a micropipette, transfer 900ul cell suspension into each of the vials. ", "Once you have filled the vials to 900ul, close all the lids, dispose of the tip and place the 1000ul pipette back in the rack. ", "Open the DMSO bottle and using a 200ul pipette, you will need to transfer 100ul to each vial using the technique outlined next.", "Add 100ul to each vial 1 by 1, ensuring you perform a quick mix/flush up and down in the pipette each time. Change tip before going back into the DMSO and following the addition of DMSO to each vial, place it immediately into the cool cell container. ", "You want to perform these steps as rapidly and efficiently as possible to avoid the cells being left in DMSO for too much time. ", "As soon as all the cryovials are full of cell suspension (90% FBS, 10% DMSO) and have been added to the cool cell, cover with the cool cell lid and place into the -80.", "The cool cell will regulate the gradual freezing of the cells ready to be placed in liquid nitrogen. " ], "layout": { "id": "main", "name": "Main grid", "x": 0, "y": 0, "w": 40, "h": 40, "ueBlueprint": "", "relativeRotation": 0, "absoluteRotation": 0, "isSurface": true, "requiresSurface": false, "items": [ { "id": "MSC_Small_0", "name": "MSC Small", "x": 0, "y": 0, "w": 28, "h": 13, "ueBlueprint": "/Game/Instruments/MSC/BP_MSC_Herasafe_Standing.BP_MSC_Herasafe_Standing_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/msc.png", "thumbnailImage": "devices/msc.png", "isSurface": true, "requiresSurface": false, "items": [ { "id": "Bottle_250ml_0", "name": "Bottle 250ml", "x": 6, "y": 2, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/GL45/BP_Bottle_GL45_250ml.BP_Bottle_GL45_250ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle02_250ml.png", "thumbnailImage": "containers/bottle02_250ml.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Bin_Sharps_0", "name": "Bin Sharps", "x": 2, "y": 2, "w": 4, 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