{ "version": "v3", "compatibility": "0.1.4", "collections": [ { "name": "Introduction to Laboratories (Secondary)", "sops": [ { "name": "Making cell media", "steps": [ "Try making cell media (cell food that helps them stay alive and multiply!).", "Grab at the square box labelled Centrifuge tube (50ml) and a tube should appear.", "Hover the tube over the rack with holes in it and let go once the blue outline appears.", "The tube should slot into place.", "Do this a few more times with more tubes until the rack is full.", "Open all of the lids on these tubes by tapping the lids.", "Grab at the long box labelled : serological pipettes (25ml).", "A long transparent pipette should appear in your hand. Keep a hold of this.", "Grab the white and blue controller with your other hand.", "Attach the end of the long pipette to the front of the controller. A blue outline should appear and the pipette should snap into place. ", "You have assembled your pipetting device.", "With you free hand tap the lid of the bottle labelled DMEM and insert the tip of the pipette into the liquid inside ready to suck it up into the pipette. ", "Now go and speak to a teacher who can show you the correct technique for pipetting using this device. ", "Practice that technique and draw up liquid to as close to 25ml as you can. Be careful not to go over that line as pulling liquid into the controller can damage it.", "Move the pipette over the tube in the rack and try releasing the liquid into one of them using the technique described to you. ", "Once you have done that you can practice sucking up different amounts of the different liquids and releasing them/mixing them in the tubes. ", "Play around with this and create different colours and concentration variations. 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", "Firstly it is important to be able to differentiate between chemicals and solutions with different hazards. ", "As part of the first exercise, group all the bottles with the same symbol on the label together.", "Any bottles with multiple symbols on the labels should be left as they are for now and not grouped.", "The exclamation mark symbol stands for 'Moderate Health Hazard' e.g. causes skin irritation. You should avoid contact with these chemicals but if contact is made with skin or eyes immediately flush the affected area with cool, running water for at least 15-20 minutes. Consider double gloving when handling these chemicals. ", "The symbol with the person and a blast on their chest stands for 'Serious Health Hazard' e.g. cause breaking difficulties. Short or long term exposure to these chemicals could be very serious and so you should avoid any contact or ingestion. If contact is made with skin or eyes immediately flush the affected area with cool, running water for at least 15-20 minutes. Consider double gloving when handling these chemicals. ", "The skull and crossbones symbol stands for 'Toxic' so could cause death if swallowed or inhaled. You should avoid contact with these chemicals but if contact is made with skin or eyes immediately flush the affected area with cool, running water for at least 15-20 minutes. Consider double gloving when handling these chemicals. ", "The symbol with a a fish and tree stands for 'Harmful to the Environment' and so it could cause damage to both animal and plant life. Responsible disposal of these chemicals is very important as they can have significant environmental impact. ", "The symbol containing liquid on hands stands for 'Corrosive' so damages skin and clothing. These chemicals may cause burns as they can even corrode metals. Do not breathe them in or inhale. You should avoid contact with these chemicals but if contact is made with skin or eyes immediately flush the affected area with cool, running water for at least 15-20 minutes. Consider double gloving when handling these chemicals. ", "The flame symbol stands for 'Flammable' and means that the substance/powder catches fire easily. Do not store them near or expose them to heat, fire or sparks. They may also give off flammable gases when exposed to water so be aware of this. ", "The symbol similar to the flammable symbol but with an O in the middle of the flame means 'Oxidizing'. These chemicals make flammable substances burn more fiercely. They also burn in the absence of air. As with flammable substances keep out of the way of heat, fire or sparks.", "'Flammables' and 'Oxidizers' should be stored separately, ideally 5 meters from each other in their own cabinet. As this SOP does not span 5 metres, for now you should place the flammable substances in the flammable cupboard away from the oxidizing chemicals, which should be placed in the oxidizers cabinet.", "*NOTE - Before storing 'Flammables' together in the cabinet it should be noted that Flammable and Corrosives should undergo a second evaluation. If a a substance is a 'flammable corrosive acid', it should not be stored with a 'flammable corrosive base'. So it is preferable to have separate cabinets for these types of flammables.", "Also consider where the oxidizers have been grouped on the benchtop, are they near any sources of heat or water? Would they be best repositioned?", "Are any of the oxidisers powder and other liquids? These should also not be stored together. ", "Now looking at 'Corrosives', as mentioned previously, acids and bases in their category cannot be stored together. You can tell if they are acids or bases by their PH. Try to separate out the acidic and basic corrosives, placing half in the one cabinet and half in the other. ", "Corrosive acids should also not be stored with anything that is a 'health hazard' or 'toxic'. ", "Using this information try your best to re-group and store chemicals together (if possible). ", "In the cases of multiple hazard pictograms on the containers, the order of importance should be considered as (from most to least): Oxidisers > Flammables > Corrosives > Toxic/Serious Health Hazard > Harzardous/Environmental Hazard.", "There are non-hazardous solutions to store as well. If you check the labels you can see if they can be stored in the fridge. ", "Any chemicals left on the bench surface are fine to do so if they have either the Harzardous/Environmental Hazard warning. ", "A teacher or training officer can assess your ability to do so by the end of this exercise. 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"containers/tromethamine_powder_100g.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Sodium_Chloride_100ml_0", "name": "Sodium Chloride 100ml", "x": 18, "y": 11, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Sodium_Chloride_100ml.BP_Bottle_Sodium_Chloride_100ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle02_100ml.png", "thumbnailImage": "containers/bottle100ml_sodium_chloride.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Tris_Buffer_100ml_0", "name": "Tris Buffer 100ml", "x": 20, "y": 11, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Tris_Buffer_100ml.BP_Bottle_Tris_Buffer_100ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle02_100ml.png", "thumbnailImage": "containers/bottle100ml_tris_buffer.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Acetate_Buffer_100ml_0", "name": "Acetate Buffer 100ml", "x": 22, "y": 11, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Acetate_Buffer_100ml.BP_Bottle_Acetate_Buffer_100ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle02_100ml.png", "thumbnailImage": "containers/bottle100ml_acetate_buffer.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Bovine_Serum_Albumin_100ml_0", "name": "Bovine Serum Albumin 100ml", "x": 24, "y": 11, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Bovine_Serum_Albumin_500ml.BP_Bottle_Bovine_Serum_Albumin_500ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle02_100ml.png", "thumbnailImage": "containers/bottle100ml_bovine_serum_albumin.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Glutamine_100ml_0", "name": "Glutamine 100ml", "x": 30, "y": 10, "w": 1, "h": 1, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Glutamine_100ml.BP_Bottle_Glutamine_100ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle03_100ml.png", "thumbnailImage": "containers/bottle100ml_glutamine.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Accutase_100ml_0", "name": "Accutase 100ml", "x": 32, "y": 10, "w": 1, "h": 1, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Accutase_100ml.BP_Bottle_Accutase_100ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle03_100ml.png", "thumbnailImage": "containers/bottle100ml_accutase.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Cell_Differentiation_Media_100ml_0", "name": "Cell Differentiation Media 100ml", "x": 34, "y": 10, "w": 1, "h": 1, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Cell_Differentiation_Media_100ml.BP_Bottle_Cell_Differentiation_Media_100ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle03_100ml.png", "thumbnailImage": "containers/bottle100ml_cell_differentiation_media.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Trypsin_EDTA_100ml_0", "name": "Trypsin EDTA 100ml", "x": 30, "y": 12, "w": 1, "h": 1, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Trypsin_100ml.BP_Bottle_Trypsin_100ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle03_100ml.png", "thumbnailImage": "containers/bottle100ml_trypsin.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Trypan_Blue_100ml_0", "name": "Trypan Blue 100ml", "x": 32, "y": 12, "w": 1, "h": 1, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Trypan_Blue_100ml.BP_Bottle_Trypan_Blue_100ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle03_100ml.png", "thumbnailImage": "containers/bottle100ml_trypan_blue.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Cryopreservation_Medium_100ml_0", "name": "Cryopreservation Medium 100ml", "x": 34, "y": 12, "w": 1, "h": 1, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Cryopresevation_Medium_100ml.BP_Bottle_Cryopresevation_Medium_100ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle03_100ml.png", "thumbnailImage": "containers/bottle100ml_cryo_medium.png", "isSurface": false, "requiresSurface": true, "items": [] } ] }, { "id": "Fridge_0", "name": "Fridge", "x": 27, "y": 26, "w": 13, "h": 14, "ueBlueprint": "/Game/Instruments/Incubators/BP_Small_Fridge_Freezer.BP_Small_Fridge_Freezer_C", "absoluteRotation": 180, "relativeRotation": 180, "gridImage": "devices/fridge_freezer_small.png", "thumbnailImage": "devices/fridge_freezer_small.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Table_120_x_45_0", "name": "Table 120 x 45", "x": 1, "y": 31, "w": 24, "h": 9, "ueBlueprint": "/Game/Instruments/Tables/BP_Table_Stand_Small_Thin.BP_Table_Stand_Small_Thin_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "furniture/table_thin.png", "thumbnailImage": "furniture/table_thin.png", "isSurface": true, "requiresSurface": true, "items": [ { "id": "Corrosives_Cabinet_0", "name": "Corrosives Cabinet", "x": 2, "y": 2, "w": 8, "h": 6, "ueBlueprint": "/Game/Instruments/HazardsCabinets/BP_Hazards_Cabinet_Corrosive.BP_Hazards_Cabinet_Corrosive_C", "absoluteRotation": 180, "relativeRotation": 180, "gridImage": "furniture/cabinet_small.png", "thumbnailImage": "furniture/cabinet_corrosives.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Oxidisers_Cabinet_0", "name": "Oxidisers Cabinet", "x": 14, "y": 2, "w": 8, "h": 6, "ueBlueprint": "/Game/Instruments/HazardsCabinets/BP_Hazards_Cabinet_Oxidiser.BP_Hazards_Cabinet_Oxidiser_C", "absoluteRotation": 180, "relativeRotation": 180, "gridImage": "furniture/cabinet_small.png", "thumbnailImage": "furniture/cabinet_oxidisers.png", "isSurface": false, "requiresSurface": true, "items": [] } ] } ], "thumbnailImage": "", "gridImage": "", "parts": [] } }, { "version": "v2", "name": "Performing a Cell Count (Microscope)", "steps": [ "Objective - Safely and accurately perform a manual cell count using a Haemocytometer and Digital Microscope. ", "Remove the Haemocytometer from the case. It features a small grid and a coverslip, between which cells are introduced and contained for counting.", "Remove a vial of cells from the dispenser ready for counting. Place it on the surface and open it by tapping the lid.", "Retrieve a microcentrifuge tube from the dispenser box. ", "Place it in the rack and pop it open.", "Retrieve a 1000ul micropipette from the pipette rack and pop a tip on the end by stabbing at the colour matched tip box.", "Insert tip into the vial of cells and using the correct pipetting action (demonstrated by the trainer), mix up and down.", "Cell mixing is important as they are not dissolved within the liquid and can settle at the bottom due to gravity. Mixing evenly distributes them and means that a representative sample can be taken. ", "Once mixed and the tip empty, hover the tip over the sharps box to remove it. The sharps box is the yellow bin on the table. ", "Return the 1000ul pipette to the pipette stand. ", "Remove a 10ul micropipette from the pipette rack and pop a tip on the end by stabbing at the colour matched tip box.", "Insert 10ul pipette tip into vial of cells suspension and withdraw 10ul.", "Once full, place the tip inside the microcentrifuge and expel the 10ul cell suspension.", "Discard of the empty tip by hovering the micropipette over and inserting the tip into the sharps bin.", "Tap the lid of the Trypan Blue bottle to open it.", "Pop a fresh tip back on the end of the 10ul micropipette by stabbing at the colour matched tip box.", "Insert 10ul pipette tip into Trypan Blue and withdraw 10ul.", "Once full, place the tip inside the microcentrifuge and expel the 10ul Trypan Blue.", "Mix the cell and Trypan Blue suspension up and down with the same pipette and then withdraw 10ul to add to the Haemocytometer.", "Load 10μl onto the Haemocytometer where the coverslip meets the counting area – the sample will be drawn underneath by capillary action.", "Dispose of the used pipette tip into the sharps bin. Close all the lids on bottles and tubes and place the micropipette back onto the pipette rack. ", "Place the assembled Haemocytometer into the slide retainer on the Microscope.", "On a real laboratory Microscope you would have it set to 10x objective (yellow ring lense) and a brightness setting of 3. ", "On a real laboratory Microscope, you would be able to navigate to the counting grid by using the repositioning knobs. This VR Microscope allows you to focus on the haemocytometer grid but the position is pre-set so the entire counting grid is visible. ", "Count at least the top left and bottom right large squares using a tally counter. Ideally you should count all four corner squares and central square in the chamber. ", "Treat cells that are more than 50% within the outer lines of the main square as countable. Count in a snaking pattern left to right within each large square, ensuring you move through all the small squares within the large square.", "In a real lab environment you would make a note of your counts and create an average of all large squares counted e.g. 3 squares = 30 cells, 30/3 = 10 cells per square. For the purpose of VR, make a mental note of your average. This is the basis for how a cell count is performed. The next steps involve finding out how many cells were in the initial vial we took the sample from. To do this:", "Multiply the result by 10,000 to convert to cells/ml in Trypan Blue", "Multiply your answer by 2 to determine the original cell concentration in the cell suspension vial (diluted 2x in Typan Blue for this protocol). ", "You have successfully performed a manual cell count on a Haemocytometer. " ], "layout": { "id": "main", "name": "Main grid", "x": 0, "y": 0, "w": 40, "h": 40, "ueBlueprint": "", "relativeRotation": 0, "absoluteRotation": 0, "isSurface": true, "requiresSurface": false, "items": [ { "id": "Table_with_Hazard_Cabinets_0", "name": "Table with Hazard Cabinets", "x": 0, "y": 7, "w": 40, "h": 15, "ueBlueprint": "/Game/Instruments/Tables/BP_Cabinet_Tabletop.BP_Cabinet_Tabletop_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "furniture/cabinets_with_table.png", "thumbnailImage": "furniture/cabinets_with_table.png", "isSurface": true, "requiresSurface": true, "items": [ { "id": "Pipettes_Mechanical_10ul,_25ul,_100ul,_1000ul_0", "name": "Pipettes Mechanical 10ul, 25ul, 100ul, 1000ul", "x": 17, "y": 3, "w": 5, "h": 3, "ueBlueprint": "/Game/Instruments/Pipettes/BP_Mechanical_Pipette_Stand.BP_Mechanical_Pipette_Stand_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/mechanical_pipette_rack.png", "thumbnailImage": "devices/mechanical_pipette_rack.png", "isSurface": false, "requiresSurface": true, "parts": [ { "slot": "Pipette10Snap", "ueBlueprint": "/Game/Instruments/Pipettes/BP_Mechanical_Pipette_Controller_10.BP_Mechanical_Pipette_Controller_10_C" }, { "slot": "Pipette20Snap", "ueBlueprint": "/Game/Instruments/Pipettes/BP_Mechanical_Pipette_Controller_20.BP_Mechanical_Pipette_Controller_20_C" }, { "slot": "Pipette200Snap", "ueBlueprint": "/Game/Instruments/Pipettes/BP_Mechanical_Pipette_Controller_200.BP_Mechanical_Pipette_Controller_200_C" }, { "slot": "Pipette1000Snap", "ueBlueprint": "/Game/Instruments/Pipettes/BP_Mechanical_Pipette_Controller_1000.BP_Mechanical_Pipette_Controller_1000_C" } ], "items": [] }, { "id": "Pipette_tip_box_20ul_0", "name": "Pipette tip box 20ul", "x": 24, "y": 3, "w": 3, "h": 3, "ueBlueprint": "/Game/Instruments/Pipettes/Dispensers/BP_Dispenser_Micro_Pipettes_20.BP_Dispenser_Micro_Pipettes_20_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": 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"requiresSurface": true, "items": [] }, { "id": "Haemocytometer_0", "name": "Haemocytometer", "x": 24, "y": 8, "w": 3, "h": 3, "ueBlueprint": "/Game/Instruments/MicroScope/BP_Haemocytometer_Case.BP_Haemocytometer_Case_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/haemocytometer.png", "thumbnailImage": "devices/haemocytometer.png", "isSurface": false, "requiresSurface": true, "parts": [ { "slot": "HmSnap", "ueBlueprint": "/Game/Instruments/MicroScope/BP_Haemocytometer.BP_Haemocytometer_C", "parts": [] } ], "items": [] }, { "id": "Tube_Rack_Micro_0", "name": "Tube Rack Micro", "x": 19, "y": 10, "w": 3, "h": 1, "ueBlueprint": "/Game/Instruments/Centrifuge/TubeRacks/BP_Centrifuge_Tube_Rack_Micro.BP_Centrifuge_Tube_Rack_Micro_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "other/tube_rack_micro.png", "thumbnailImage": "other/tube_rack_micro.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Microcentrifuge_Tube_1.5ml_0", "name": "Microcentrifuge Tube 1.5ml", "x": 19, "y": 7, "w": 3, "h": 2, "ueBlueprint": "/Game/Instruments/Centrifuge/BP_Dispenser_Microcentrifuge_Tube_1halfml.BP_Dispenser_Microcentrifuge_Tube_1halfml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/dispenser_microcentrifuge_tube_one_half_ml.png", "thumbnailImage": "containers/microcentrifuge_tube_one_half_ml.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Ethanol_Spray_Bottle_0", "name": "Ethanol Spray Bottle", "x": 2, "y": 4, "w": 3, "h": 2, "ueBlueprint": "/Game/Instruments/Sanitising/BP_Spray.BP_Spray_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "cleaning/spray_bottle.png", "thumbnailImage": "cleaning/spray_bottle.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Ethanol_Wash_Bottle_0", "name": "Ethanol Wash Bottle", "x": 2, "y": 7, "w": 3, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/SqueezeBottles/BP_Squeeze_Bottle_Ethanol.BP_Squeeze_Bottle_Ethanol_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/wash_bottle_ethanol_5l.png", "thumbnailImage": "containers/wash_bottle_ethanol_5l.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Wipes_IPA_0", "name": "Wipes IPA", "x": 1, "y": 10, "w": 5, "h": 3, "ueBlueprint": "/Game/Instruments/Sanitising/BP_Dispenser_Wipes.BP_Dispenser_Wipes_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "cleaning/wipes_ipa.png", "thumbnailImage": "cleaning/wipes_ipa.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Microscope_Digital_0", "name": "Microscope Digital", "x": 7, "y": 3, "w": 9, "h": 7, "ueBlueprint": "/Game/Instruments/MicroScope/BP_Digital_Microscope.BP_Digital_Microscope_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/microscope_digital.png", "thumbnailImage": "devices/microscope_digital.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Trypan_Blue_100ml_0", "name": "Trypan Blue 100ml", "x": 16, "y": 9, "w": 1, "h": 1, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Trypan_Blue_100ml.BP_Bottle_Trypan_Blue_100ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle03_100ml.png", "thumbnailImage": "containers/bottle100ml_trypan_blue.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Pipette_tip_box_1000ul_0", "name": "Pipette tip box 1000ul", "x": 30, "y": 3, "w": 3, "h": 3, "ueBlueprint": "/Game/Instruments/Pipettes/Dispensers/BP_Dispenser_Micro_Pipettes_1000.BP_Dispenser_Micro_Pipettes_1000_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/dispenser_pipette_tips_1000ul.png", "thumbnailImage": "containers/dispenser_pipette_tips_1000ul.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Bin_Sharps_0", "name": "Bin Sharps", "x": 34, "y": 8, "w": 4, "h": 4, "ueBlueprint": "/Game/Instruments/Bins/BP_Bin_Sharps.BP_Bin_Sharps_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "cleaning/bin_sharps.png", "thumbnailImage": "cleaning/bin_sharps.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Pipette_tip_box_200ul_0", "name": "Pipette tip box 200ul", "x": 33, "y": 3, "w": 3, "h": 3, "ueBlueprint": "/Game/Instruments/Pipettes/Dispensers/BP_Dispenser_Micro_Pipettes_200.BP_Dispenser_Micro_Pipettes_200_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/dispenser_pipette_tips_200ul.png", "thumbnailImage": "containers/dispenser_pipette_tips_200ul.png", "isSurface": false, "requiresSurface": true, "items": [] } ] } ], "thumbnailImage": "", "gridImage": "", "parts": [] } }, { "name": "Precision weighing and measuring", "steps": [ "Purpose and Scope: Initial training into the calibration of a weighing scale prior to weighing liquids for accuracy post pipetting. ", "Check for visible damage, loose components, or debris on the weighing platform.", "Ensure the draft shield door on the scales is closed. ", "Turn on the analytical scale and allow it to stabilise.", "Check the scale is balanced by inspecting the indicator. The bubble should be in the centre of the indicator ring. If not this can be adjusted by tapping each wheel. ", "Check that the measurement mode is in grams.", "Press the Function then Tare button to access to the calibration screen.", "Gently open the draft field door.", "Pick up the smallest/lightest calibration weight from the box and place in the centre of the scale. ", "Gently close the draft field door by touching the handle.", "The scale should show weight but wait a few seconds until balance settles on a weight.", "Make a note of the weight in a calibration book.", "Gently open the draft field door by touching the handle.", "Remove the weight from the scale and close the draft field door.", "Re-TARE the scale.", "Repeat the calibration/calibration weight weighing process with the other weights in order lightest-heaviest.", "Once all the weights have been calibrated , double tap the function button to take you back to the sample measuring mode.", "Press the TARE button. ", "Gently open the draft field door. ", "Place the empty 50ml centrifuge tube on the scale and gently close the draft field door. ", "Make a note of the weight pre-liquid. ", "Press TARE. ", "Gently open the draft field door and remove the empty centrifuge tube. ", "Gently close the draft field door. ", "Using a serological pipette and pipette controller, transfer 36ml FBS into the centrifuge tube. ", "Gently open the draft field door. ", "Place the filled 50ml centrifuge tube on the scale and gently close the draft field door. ", "Make a note of the weight post FBS. ", "Gently open the draft field door and remove the filled centrifuge tube. ", "Gently close the draft field door.", "Using a 1000ul mechanical pipette and 1000ul tips, transfer 4ml of DMSO 1ml by 1ml pipetting up and down each time to mix, into the centrifuge tube to bring the total volume to 40ml. ", "Gently open the draft field door.", "Place the filled 50ml centrifuge tube on the scale and gently close the draft field door.", "Make a note of the weight post FBS&DMSO to determine if the final weight corresponds to the total volume.", "Gently open the draft field door and remove the filled centrifuge tube.", "Close the draft field door.", "Turn off the scales." ], "layout": { "id": "main", "name": "Main grid", "x": 0, "y": 0, "w": 40, "h": 40, "ueBlueprint": "", "relativeRotation": 0, "absoluteRotation": 0, "isSurface": true, "requiresSurface": false, "items": [ { "id": "Table_200_x_70_0", "name": "Table 200 x 70", "x": 0, "y": 1, "w": 40, "h": 14, "ueBlueprint": "/Game/Instruments/Tables/BP_Table_200x70.BP_Table_200x70_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "furniture/table_200x70.png", "thumbnailImage": "furniture/table_large.png", "isSurface": true, "requiresSurface": true, "items": [ { "id": "Dispenser_Paper_Table_0", "name": "Dispenser Paper Table", "x": 2, "y": 1, "w": 4, "h": 4, "ueBlueprint": "/Game/Instruments/Sanitising/BP_Dispenser_Paper_Towel.BP_Dispenser_Paper_Towel_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "cleaning/dispenser_paper_table.png", "thumbnailImage": "cleaning/dispenser_paper_table.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Wipes_IPA_0", "name": "Wipes IPA", "x": 2, "y": 6, "w": 5, "h": 3, "ueBlueprint": "/Game/Instruments/Sanitising/BP_Dispenser_Wipes.BP_Dispenser_Wipes_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "cleaning/wipes_ipa.png", "thumbnailImage": "cleaning/wipes_ipa.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Ethanol_Spray_Bottle_0", "name": "Ethanol Spray Bottle", "x": 3, "y": 10, "w": 3, "h": 2, "ueBlueprint": "/Game/Instruments/Sanitising/BP_Spray.BP_Spray_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "cleaning/spray_bottle.png", "thumbnailImage": "cleaning/spray_bottle.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Analytical_Scale_0", "name": "Analytical Scale", "x": 9, "y": 1, "w": 7, "h": 7, "ueBlueprint": "/Game/Instruments/Measuring/WeighingScale/BP_Analytical_Scale.BP_Analytical_Scale_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/analytical_scale.png", "thumbnailImage": "devices/analytical_scale.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Analytical_Scale_-_Calibration_Weights_0", "name": "Analytical Scale - 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", "Pinch at the box labelled 'antibodies' to remove a tube and place it in the other rack.", "Pick up the blue micropipette from the stand of pipettes by grabbing at it. ", "Insert the blue pipette into the pipette tip box to pick up a tip. ", "Adjust the 1000uL micropipette volume to 900uL using the + and - toggles.", "Dip the tip of the pipette inside the flow buffer solution and withdraw it into the pipette.", "Once the numbers on the pop up above the pipette reaches 900uL, you can move the pipette from the bottle and push the liquid out into the top left micropipette in the rack.", "Return the blue 1000uL pipette and retrieve the yellow 200uL pipette from the rack", "Adjust the 200uL micropipette volume to 100uL using the + and - toggles.", "Dip the tip of the pipette inside the antibody vial and withdraw it into the pipette.", "Dispense 100uL antibodies into the top left micropipette in the rack with the flow buffer to create a 1:10 dilution. You should label this tube 1:10 in the real lab. ", "Remove the tip and close the tube lid.", "Vortex the tube for 5-10 seconds to mix thoroughly.", "Retrieve a new tip for the 200uL micropipette", "Remove 100uL of the 1:10 solution from the first microcentrifuge tube and transfer to the next tube along (tube 2) which in the lab would be labelled 1:100. ", "Retrieving a new pipette tip for the 1000uL pipette, adjust to 900uL and transfer 900uL flow buffer into the 1:100 tube (tube 2).", "Vortex the tube for 5-10 seconds to mix thoroughly.", "Repeat this process for as many dilutions as needed (e.g. 1:1000, 1:10000), each time transferring 100 uL from the previous dilution to a new micro tube with 900 uL of flow buffer and vortexing to mix.", "In the laboratory flow buffer-antibody staining solutions of different dilutions would be added to cells before performing the phenotyping. Running the antibody at different dilutions (a “titration”) helps determine the best working concentration for that specific antibody, cell type and experiment." ], "layout": { "id": "main", "name": "Main grid", "x": 0, "y": 0, "w": 40, "h": 40, "ueBlueprint": "", "relativeRotation": 0, "absoluteRotation": 0, "isSurface": true, "requiresSurface": false, "items": [ { "id": "Table_180_x_60_0", "name": "Table 180 x 60", "x": 2, "y": 5, "w": 36, "h": 12, "ueBlueprint": "/Game/Instruments/Tables/BP_Table_180x60.BP_Table_180x60_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "furniture/table_180x60.png", "thumbnailImage": "furniture/table_large.png", "isSurface": true, "requiresSurface": true, "items": [ { "id": "Bio_Bin_1L_0", "name": "Bio Bin 1L", "x": 30, "y": 1, "w": 5, "h": 4, "ueBlueprint": "/Game/Instruments/Bins/BP_Bin_1L_Bio.BP_Bin_1L_Bio_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "cleaning/bio_bin_1l.png", "thumbnailImage": 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[] }, { "id": "Tube_Rack_Micro_0", "name": "Tube Rack Micro", "x": 13, "y": 8, "w": 3, "h": 1, "ueBlueprint": "/Game/Instruments/Centrifuge/TubeRacks/BP_Centrifuge_Tube_Rack_Micro.BP_Centrifuge_Tube_Rack_Micro_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "other/tube_rack_micro.png", "thumbnailImage": "other/tube_rack_micro.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Tube_Rack_Micro_1", "name": "Tube Rack Micro", "x": 13, "y": 9, "w": 3, "h": 1, "ueBlueprint": "/Game/Instruments/Centrifuge/TubeRacks/BP_Centrifuge_Tube_Rack_Micro.BP_Centrifuge_Tube_Rack_Micro_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "other/tube_rack_micro.png", "thumbnailImage": "other/tube_rack_micro.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Tube_Rack_15ml_1", "name": "Tube Rack 15ml", "x": 22, "y": 9, "w": 3, "h": 1, "ueBlueprint": 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