{ "version": "v3", "compatibility": "0.1.4", "collections": [ { "name": "Fundamental Lab Skills", "sops": [ { "name": "Weighing liquids ", "steps": [ "Purpose and Scope: Initial training into weighing liquids for accuracy post pipetting. ", "Check for visible damage, loose components, or debris on the weighing platform.", "Ensure the draft shield door on the scales is closed. ", "Turn on the analytical scale and allow it to stabilise.", "Check the scale is balanced by inspecting the indicator. The bubble should be in the centre of the indicator ring. If not this can be adjusted by tapping each wheel. ", "Check that the measurement mode is in grams.", "Press the Function then Tare button to access to the calibration screen.", "Gently open the draft field door.", "Pick up the smallest/lightest calibration weight from the box and place in the centre of the scale. ", "Gently close the draft field door by touching the handle.", "The scale should show weight but wait a few seconds until balance settles on a weight.", "Make a note of the weight in a calibration book.", "Gently open the draft field door by touching the handle.", "Remove the weight from the scale and close the draft field door.", "Re-TARE the scale.", "Repeat the calibration/calibration weight weighing process with the other weights in order lightest-heaviest.", "Once all the weights have been calibrated , double tap the function button to take you back to the sample measuring mode.", "Press the TARE button. ", "Gently open the draft field door. ", "Place the empty 50ml centrifuge tube on the scale and gently close the draft field door. ", "Make a note of the weight pre-liquid. ", "Press TARE. ", "Gently open the draft field door and remove the empty centrifuge tube. ", "Gently close the draft field door. ", "Using a serological pipette and pipette controller, transfer 36ml FBS into the centrifuge tube. ", "Gently open the draft field door. ", "Place the filled 50ml centrifuge tube on the scale and gently close the draft field door. ", "Make a note of the weight post FBS. ", "If the weight is higher or lower than 36 then remove or add FBS using a mechanical pipette until it stabilises at the target value. ", "Gently open the draft field door and remove the filled centrifuge tube. ", "Gently close the draft field door.", "Using a 1000ul mechanical pipette and 1000ul tips, transfer 4ml of DMSO 1ml by 1ml pipetting up and down each time to mix, into the centrifuge tube to bring the total volume to 40ml. ", "Gently open the draft field door.", "Place the filled 50ml centrifuge tube on the scale and gently close the draft field door.", "Make a note of the weight post FBS&DMSO to determine if the final weight corresponds to the total volume.", "Gently open the draft field door and remove the filled centrifuge tube.", "Close the draft field door.", "Turn off the scales." ], "layout": { "id": "main", "name": "Main grid", "x": 0, "y": 0, "w": 40, "h": 40, "ueBlueprint": "", "relativeRotation": 0, "absoluteRotation": 0, "isSurface": true, "requiresSurface": false, "items": [ { "id": "Table_200_x_70_0", "name": "Table 200 x 70", "x": 0, "y": 1, "w": 40, "h": 14, "ueBlueprint": "/Game/Instruments/Tables/BP_Table_200x70.BP_Table_200x70_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "furniture/table_200x70.png", "thumbnailImage": "furniture/table_large.png", "isSurface": true, "requiresSurface": true, "items": [ { "id": "Dispenser_Paper_Table_0", "name": "Dispenser Paper Table", "x": 2, "y": 1, "w": 4, "h": 4, "ueBlueprint": "/Game/Instruments/Sanitising/BP_Dispenser_Paper_Towel.BP_Dispenser_Paper_Towel_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "cleaning/dispenser_paper_table.png", "thumbnailImage": "cleaning/dispenser_paper_table.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Wipes_IPA_0", "name": "Wipes IPA", "x": 2, "y": 6, "w": 5, "h": 3, "ueBlueprint": "/Game/Instruments/Sanitising/BP_Dispenser_Wipes.BP_Dispenser_Wipes_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "cleaning/wipes_ipa.png", "thumbnailImage": "cleaning/wipes_ipa.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Ethanol_Spray_Bottle_0", "name": "Ethanol Spray Bottle", "x": 3, "y": 10, "w": 3, "h": 2, "ueBlueprint": "/Game/Instruments/Sanitising/BP_Spray.BP_Spray_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "cleaning/spray_bottle.png", "thumbnailImage": "cleaning/spray_bottle.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Analytical_Scale_0", "name": "Analytical Scale", "x": 9, "y": 1, "w": 7, "h": 7, "ueBlueprint": "/Game/Instruments/Measuring/WeighingScale/BP_Analytical_Scale.BP_Analytical_Scale_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/analytical_scale.png", "thumbnailImage": "devices/analytical_scale.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Analytical_Scale_-_Calibration_Weights_0", "name": "Analytical Scale - Calibration Weights", "x": 17, "y": 1, "w": 6, "h": 2, "ueBlueprint": "/Game/Instruments/Measuring/WeighingScale/CalibrationWeights/BP_Calibration_Weights_Box.BP_Calibration_Weights_Box_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/analytical_scale_weights.png", "thumbnailImage": "devices/analytical_scale_weights.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Pipette_tip_box_1000ul_0", "name": "Pipette tip box 1000ul", "x": 24, "y": 1, "w": 3, "h": 3, "ueBlueprint": "/Game/Instruments/Pipettes/Dispensers/BP_Dispenser_Micro_Pipettes_1000.BP_Dispenser_Micro_Pipettes_1000_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/dispenser_pipette_tips_1000ul.png", "thumbnailImage": "containers/dispenser_pipette_tips_1000ul.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Pipette_Controller_0", "name": "Pipette Controller", "x": 31, "y": 5, "w": 1, "h": 4, "ueBlueprint": "/Game/Instruments/Pipettes/BP_Pipette_Controller.BP_Pipette_Controller_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/pipette_controller.png", "thumbnailImage": "devices/pipette_controller.png", "isSurface": false, "requiresSurface": false, "items": [] }, { "id": "Bin_Sharps_0", "name": "Bin Sharps", "x": 35, "y": 1, "w": 4, "h": 4, "ueBlueprint": "/Game/Instruments/Bins/BP_Bin_Sharps.BP_Bin_Sharps_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "cleaning/bin_sharps.png", "thumbnailImage": "cleaning/bin_sharps.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Pipettes_Mechanical_10ul,_25ul,_100ul,_1000ul_0", "name": "Pipettes Mechanical 10ul, 25ul, 100ul, 1000ul", "x": 28, "y": 1, "w": 5, "h": 3, "ueBlueprint": "/Game/Instruments/Pipettes/BP_Mechanical_Pipette_Stand.BP_Mechanical_Pipette_Stand_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/mechanical_pipette_rack.png", "thumbnailImage": "devices/mechanical_pipette_rack.png", "isSurface": false, "requiresSurface": true, "parts": [ { "slot": "Pipette10Snap", "ueBlueprint": "/Game/Instruments/Pipettes/BP_Mechanical_Pipette_Controller_10.BP_Mechanical_Pipette_Controller_10_C" }, { "slot": "Pipette20Snap", "ueBlueprint": "/Game/Instruments/Pipettes/BP_Mechanical_Pipette_Controller_20.BP_Mechanical_Pipette_Controller_20_C" }, { "slot": "Pipette200Snap", "ueBlueprint": "/Game/Instruments/Pipettes/BP_Mechanical_Pipette_Controller_200.BP_Mechanical_Pipette_Controller_200_C" }, { "slot": "Pipette1000Snap", "ueBlueprint": "/Game/Instruments/Pipettes/BP_Mechanical_Pipette_Controller_1000.BP_Mechanical_Pipette_Controller_1000_C" } ], "items": [] }, { "id": "Fetal_Bovine_Serum_(FBS)_1L_0", "name": "Fetal Bovine Serum (FBS) 1L", "x": 20, "y": 6, "w": 3, "h": 3, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_FBS_1L.BP_Bottle_FBS_1L_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle02_1l.png", "thumbnailImage": "containers/bottle1l_fbs.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "DMSO_100ml_0", "name": "DMSO 100ml", "x": 25, "y": 7, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_DMSO_100ml.BP_Bottle_DMSO_100ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle02_100ml.png", "thumbnailImage": "containers/bottle100ml_dmso.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Centrifuge_Tube_50ml_0", "name": "Centrifuge Tube 50ml", "x": 34, "y": 6, "w": 4, "h": 3, "ueBlueprint": "/Game/Instruments/Centrifuge/BP_Dispenser_Centrifuge_Tube_50ml.BP_Dispenser_Centrifuge_Tube_50ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/dispenser_centrifuge_tube_50ml.png", "thumbnailImage": "containers/centrifuge_tube_50ml.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Seropipette_25ml_0", "name": "Seropipette 25ml", "x": 30, "y": 10, "w": 8, "h": 1, "ueBlueprint": "/Game/Instruments/Pipettes/Dispensers/BP_Dispenser_Seropips_25ml.BP_Dispenser_Seropips_25ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/dispenser_seropipette_25ml.png", "thumbnailImage": "containers/seropip_25ml.png", "isSurface": false, "requiresSurface": true, "items": [] } ] }, { "id": "Bio_Bin_6L_0", "name": "Bio Bin 6L", "x": 35, "y": 16, "w": 5, "h": 4, "ueBlueprint": "/Game/Instruments/Bins/BP_Bin_6L_Bio.BP_Bin_6L_Bio_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "cleaning/bio_bin_6l.png", "thumbnailImage": "cleaning/bio_bin_6l.png", "isSurface": false, "requiresSurface": true, "items": [] } ], "thumbnailImage": "", "gridImage": "", "parts": [] } }, { "name": "Working with Hazardous Chemicals", "steps": [ "The aim of this protocol is to understand how to properly handle chemicals. You should perform this protocol after looking at the general health and safety protocol. ", "Firstly it is important to be able to differentiate between chemicals and solutions with different hazards. ", "Hazards can either pose a risk: directly to you (e.g. by skin contact), indirectly to you and others (e.g. by reaction or spillage) or to the environment (e.g. by pollution of water).", "Measures should be taken in the laboratory to avoid any potential dangers and minimize likelihood of/exposure to these hazards. ", "There are 2 main tools that are used to identify chemical hazards: safety data sheets and chemical labels. ", "You can find some examples of safety data sheets (SDS) and other chemical/hazard identification resources on the website page for this training package. It is important that you take a look at these as the SDS is a one-stop-shop to find out about a chemical's properties, hazards and safety precautions. ", "You should know where to find the SDSs in your laboratory and read them before working with a chemical for the first time. ", "Following this you should produce a risk assessment prior to working with those chemicals/hazardous substances.", "For now we are going to look at the Globally Harmonised System (GHS) of classification and labeling if chemicals. ", "Within the laboratory, before handing any of these chemicals you should ensure: your hair is tied back, you are wearing correct personal protective equipment (PPE) e.g. a closed lab coat and closed shoes, safety goggles and a double layer of gloves.", "Pick up the bottles one by one and take a look at the labels. ", "The exclamation mark symbol stands for 'Moderate Health Hazard' e.g. causes skin irritation. You should avoid contact with these chemicals but if contact is made with skin or eyes immediately flush the affected area with cool, running water for at least 15-20 minutes.", "The symbol with the person and a blast on their chest stands for 'Serious Health Hazard' e.g. cause breaking difficulties. Short or long term exposure to these chemicals could be very serious and so you should avoid any contact or ingestion. If contact is made with skin or eyes immediately flush the affected area with cool, running water for at least 15-20 minutes. ", "The skull and crossbones symbol stands for 'Toxic' so could cause death if swallowed or inhaled. You should avoid contact with these chemicals but if contact is made with skin or eyes immediately flush the affected area with cool, running water for at least 15-20 minutes. ", "The symbol containing liquid on hands stands for 'Corrosive' so damages skin and clothing. These chemicals may cause burns as they can even corrode metals. Do not breathe them in or inhale. You should avoid contact with these chemicals but if contact is made with skin or eyes immediately flush the affected area with cool, running water for at least 15-20 minutes. ", "You should avoid pouring liquid from these chemicals as it is more likely to result in spillage. ", "Instead use the appropriate pipetting technique.", "You should also always avoid touching your face when handling these chemicals.", "The symbol with a a fish and tree stands for 'Harmful to the Environment' and so it could cause damage to both animal and plant life. ", "Responsible disposal of these chemicals is very important as they can have significant environmental impact. ", "To understand better how to properly dispose of these chemicals, take a look at the 'Waste Disposal' protocol in this collection. ", "The flame symbol stands for 'Flammable' and means that the substance/powder catches fire easily. They may also give off flammable gases when exposed to water so be aware of this. ", "The symbol similar to the flammable symbol but with an O in the middle of the flame means 'Oxidizing'. These chemicals make flammable substances burn more fiercely. They also burn in the absence of air. ", "It is critical to remember when working with 'flammables' and 'oxidisers' to not store them near or expose them to heat, fire or sparks. ", "'Flammables' and 'Oxidizers' should be stored separately, ideally 5 meters from each other in their own cabinet. This is because they can react violently with each other. ", "In addition, 'corrosive acid's' and 'corrosive bases' should also be stored and kept separately as they can react violently with each other. ", "You can tell if the chemicals are acidic or basic by their PH's.", "Corrosive acids should also not be stored with anything that is a 'health hazard' or 'toxic' due to the potential for dangerous reactions and the release of harmful substances.", "In the cases of multiple hazard pictograms on the containers, the order of importance should be considered as (from most to least): Oxidisers > Flammables > Corrosives > Toxic/Serious Health Hazard > Harzardous/Environmental Hazard.", "", "", "" ], "layout": { "id": "main", "name": "Main grid", "x": 0, "y": 0, "w": 40, "h": 40, "ueBlueprint": "", "relativeRotation": 0, "absoluteRotation": 0, "isSurface": true, "requiresSurface": false, "items": [ { "id": "Table_with_Hazard_Cabinets_0", "name": "Table with Hazard Cabinets", "x": 0, "y": 0, "w": 40, "h": 15, "ueBlueprint": "/Game/Instruments/Tables/BP_Cabinet_Tabletop.BP_Cabinet_Tabletop_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "furniture/cabinets_with_table.png", "thumbnailImage": "furniture/cabinets_with_table.png", "isSurface": true, "requiresSurface": true, "items": [ { "id": "Hotplate_Stirrer_0", "name": "Hotplate Stirrer", "x": 33, "y": 2, "w": 5, "h": 7, "ueBlueprint": "/Game/Instruments/HotPlateStirrer/BP_Hot_Plate_Stirrer.BP_Hot_Plate_Stirrer_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/hotplate_stirrer.png", "thumbnailImage": "devices/hotplate_stirrer.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Water_Bath_0", "name": "Water Bath", "x": 21, "y": 2, "w": 11, "h": 7, "ueBlueprint": "/Game/Instruments/WaterBath/BP_Water_Bath.BP_Water_Bath_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/water_bath.png", "thumbnailImage": "devices/water_bath.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Water_Wash_Bottle_0", "name": "Water Wash Bottle", "x": 2, "y": 11, "w": 3, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/SqueezeBottles/BP_Squeeze_Bottle_Water.BP_Squeeze_Bottle_Water_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/wash_bottle_water_5l.png", "thumbnailImage": "containers/wash_bottle_water_5l.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Ethanol_Wash_Bottle_0", "name": "Ethanol Wash Bottle", "x": 10, "y": 11, "w": 3, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/SqueezeBottles/BP_Squeeze_Bottle_Ethanol.BP_Squeeze_Bottle_Ethanol_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/wash_bottle_ethanol_5l.png", "thumbnailImage": "containers/wash_bottle_ethanol_5l.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Isopropanol_Wash_Bottle_0", "name": "Isopropanol Wash Bottle", "x": 6, "y": 11, "w": 3, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/SqueezeBottles/BP_Squeeze_Bottle_Isopropanol.BP_Squeeze_Bottle_Isopropanol_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/wash_bottle_isopropanol_5l.png", "thumbnailImage": "containers/wash_bottle_isopropanol_5l.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Sterile_PBS_1L_0", "name": "Sterile PBS 1L", "x": 17, "y": 2, "w": 3, "h": 3, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_PBS_1L.BP_Bottle_PBS_1L_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle01_1l.png", "thumbnailImage": "containers/bottle1l_sterile_pbs.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Distilled_Water_1L_0", "name": "Distilled Water 1L", "x": 17, "y": 5, "w": 3, "h": 3, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Distilled_Water_1L.BP_Bottle_Distilled_Water_1L_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle01_1l.png", "thumbnailImage": "containers/bottle1l_distilled_water.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Acrylamide_1L_0", "name": "Acrylamide 1L", "x": 1, "y": 1, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Acrylamide_1L.BP_Bottle_Acrylamide_1L_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle06_1l.png", "thumbnailImage": "containers/bottle1l_acrylamide.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Ethanol_1L_0", "name": "Ethanol 1L", "x": 4, "y": 1, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Ethanol_1L.BP_Bottle_Ethanol_1L_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle06_1l.png", "thumbnailImage": "containers/bottle1l_alcohol.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Formaldehyde_1L_0", "name": "Formaldehyde 1L", "x": 7, "y": 1, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Formaldehyde_1L.BP_Bottle_Formaldehyde_1L_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle06_1l.png", "thumbnailImage": "containers/bottle1l_formaldehyde.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Glutaraldehyde_1L_0", "name": "Glutaraldehyde 1L", "x": 10, "y": 1, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Glutaraldehyde_1L.BP_Bottle_Glutaraldehyde_1L_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle06_1l.png", "thumbnailImage": "containers/bottle1l_glutaraldehyde.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Hydrochloric_Acid_1L_0", "name": "Hydrochloric Acid 1L", "x": 13, "y": 1, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Hydrochloric_Acid_1L.BP_Bottle_Hydrochloric_Acid_1L_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle06_1l.png", "thumbnailImage": "containers/bottle1l_hydrochloric_acid.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Hydrogen_Peroxide_1L_0", "name": "Hydrogen Peroxide 1L", "x": 1, "y": 4, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Hydrogen_Peroxide_1L.BP_Bottle_Hydrogen_Peroxide_1L_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle06_1l.png", "thumbnailImage": "containers/bottle1l_hydrogen_peroxide.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "IMS_1L_0", "name": "IMS 1L", "x": 4, "y": 4, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_IMS_1L.BP_Bottle_IMS_1L_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle06_1l.png", "thumbnailImage": "containers/bottle1l_ims.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Nitric_Acid_1L_0", "name": "Nitric Acid 1L", "x": 7, "y": 4, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Nitric_Acid_1L.BP_Bottle_Nitric_Acid_1L_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle06_1l.png", "thumbnailImage": "containers/bottle1l_nitric_acid.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "IPA_1L_0", "name": "IPA 1L", "x": 10, "y": 4, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_IPA_1L.BP_Bottle_IPA_1L_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle06_1l.png", "thumbnailImage": "containers/bottle1l_ipa.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Sodium_Hydroxide_1L_0", "name": "Sodium Hydroxide 1L", "x": 13, "y": 4, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Sodium_Hydroxide_1L.BP_Bottle_Sodium_Hydroxide_1L_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle06_1l.png", "thumbnailImage": "containers/bottle1l_sodium_hydroxide.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Potassium_Hydroxide_1L_0", "name": "Potassium Hydroxide 1L", "x": 1, "y": 7, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Potassium_Hydroxide_1L.BP_Bottle_Potassium_Hydroxide_1L_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle06_1l.png", "thumbnailImage": "containers/bottle1l_potassium_hydroxide.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Sulphuric_Acid_1L_0", "name": "Sulphuric Acid 1L", "x": 4, "y": 7, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Sulphuric_Acid_1L.BP_Bottle_Sulphuric_Acid_1L_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle06_1l.png", "thumbnailImage": "containers/bottle1l_sulphuric_acid.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Xylene_1L_0", "name": "Xylene 1L", "x": 7, "y": 7, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Xylene_1L.BP_Bottle_Xylene_1L_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle06_1l.png", "thumbnailImage": "containers/bottle1l_xylene.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Copper_Sulfate_100ml_0", "name": "Copper Sulfate 100ml", "x": 10, "y": 7, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Copper_Sulfate_100ml.BP_Bottle_Copper_Sulfate_100ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle02_100ml.png", "thumbnailImage": "containers/bottle100ml_copper_sulfate.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Cell_Lysis_Buffer_100ml_0", "name": "Cell Lysis Buffer 100ml", "x": 12, "y": 7, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Cell_Lysis_Buffer_100ml.BP_Bottle_Cell_Lysis_Buffer_100ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle02_100ml.png", "thumbnailImage": "containers/bottle100ml_cell_lysis_buffer.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "DMSO_100ml_0", "name": "DMSO 100ml", "x": 14, "y": 7, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_DMSO_100ml.BP_Bottle_DMSO_100ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle02_100ml.png", "thumbnailImage": "containers/bottle100ml_dmso.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Flow_Buffer_100ml_0", "name": "Flow Buffer 100ml", "x": 14, "y": 9, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Flow_Buffer_100ml.BP_Bottle_Flow_Buffer_100ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle02_100ml.png", "thumbnailImage": "containers/bottle100ml_flow_buffer.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Paraformaldehyde_Fixation_Buffer_100ml_0", "name": "Paraformaldehyde Fixation Buffer 100ml", "x": 16, "y": 9, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_PFA_100ml.BP_Bottle_PFA_100ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle02_100ml.png", "thumbnailImage": "containers/bottle100ml_paraformaldehyde_fixation_buffer.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "HEPES_Powder_100g_0", "name": "HEPES Powder 100g", "x": 18, "y": 9, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Powders/BP_HEPES_100g.BP_HEPES_100g_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle02_100ml.png", "thumbnailImage": "containers/hepes_powder_100g.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Potassium_Chloride_Powder_100g_0", "name": "Potassium Chloride Powder 100g", "x": 20, "y": 9, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Powders/BP_Potassium_Chloride_100g.BP_Potassium_Chloride_100g_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle02_100ml.png", "thumbnailImage": "containers/potassium_chloride_powder_100g.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Potassium_Nitrate_Powder_100g_0", "name": "Potassium Nitrate Powder 100g", "x": 22, "y": 9, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Powders/BP_Potassium_Nitrate_100g.BP_Potassium_Nitrate_100g_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle02_100ml.png", "thumbnailImage": "containers/potassium_nitrate_powder_100g.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Sodium_Chloride_Powder_100g_0", "name": "Sodium Chloride Powder 100g", "x": 24, "y": 9, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Powders/BP_Sodium_Chloride_100g.BP_Sodium_Chloride_100g_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle02_100ml.png", "thumbnailImage": "containers/sodium_chloride_powder_100g.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Potassium_Nitrate_100ml_0", "name": "Potassium Nitrate 100ml", "x": 26, "y": 9, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Potassium_Nitrate_100ml.BP_Bottle_Potassium_Nitrate_100ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle02_100ml.png", "thumbnailImage": "containers/bottle100ml_potassium_nitrate.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Sodium_Hydroxide_Powder_100g_0", "name": "Sodium Hydroxide Powder 100g", "x": 14, "y": 11, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Powders/BP_Sodium_Hydroxide_100g.BP_Sodium_Hydroxide_100g_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle02_100ml.png", "thumbnailImage": "containers/sodium_hydroxide_powder_100g.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Tromethamine_Powder_100g_0", "name": "Tromethamine Powder 100g", "x": 16, "y": 11, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Powders/BP_Tromethamine_100g.BP_Tromethamine_100g_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle02_100ml.png", "thumbnailImage": "containers/tromethamine_powder_100g.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Sodium_Chloride_100ml_0", "name": "Sodium Chloride 100ml", "x": 18, "y": 11, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Sodium_Chloride_100ml.BP_Bottle_Sodium_Chloride_100ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle02_100ml.png", "thumbnailImage": "containers/bottle100ml_sodium_chloride.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Tris_Buffer_100ml_0", "name": "Tris Buffer 100ml", "x": 20, "y": 11, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Tris_Buffer_100ml.BP_Bottle_Tris_Buffer_100ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle02_100ml.png", "thumbnailImage": "containers/bottle100ml_tris_buffer.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Acetate_Buffer_100ml_0", "name": "Acetate Buffer 100ml", "x": 22, "y": 11, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Acetate_Buffer_100ml.BP_Bottle_Acetate_Buffer_100ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle02_100ml.png", "thumbnailImage": "containers/bottle100ml_acetate_buffer.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Bovine_Serum_Albumin_100ml_0", "name": "Bovine Serum Albumin 100ml", "x": 24, "y": 11, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Bovine_Serum_Albumin_500ml.BP_Bottle_Bovine_Serum_Albumin_500ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle02_100ml.png", "thumbnailImage": "containers/bottle100ml_bovine_serum_albumin.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Glutamine_100ml_0", "name": "Glutamine 100ml", "x": 30, "y": 10, "w": 1, "h": 1, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Glutamine_100ml.BP_Bottle_Glutamine_100ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle03_100ml.png", "thumbnailImage": "containers/bottle100ml_glutamine.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Accutase_100ml_0", "name": "Accutase 100ml", "x": 32, "y": 10, "w": 1, "h": 1, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Accutase_100ml.BP_Bottle_Accutase_100ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle03_100ml.png", "thumbnailImage": "containers/bottle100ml_accutase.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Cell_Differentiation_Media_100ml_0", "name": "Cell Differentiation Media 100ml", "x": 34, "y": 10, "w": 1, "h": 1, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Cell_Differentiation_Media_100ml.BP_Bottle_Cell_Differentiation_Media_100ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle03_100ml.png", "thumbnailImage": "containers/bottle100ml_cell_differentiation_media.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Trypsin_EDTA_100ml_0", "name": "Trypsin EDTA 100ml", "x": 30, "y": 12, "w": 1, "h": 1, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Trypsin_100ml.BP_Bottle_Trypsin_100ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle03_100ml.png", "thumbnailImage": "containers/bottle100ml_trypsin.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Trypan_Blue_100ml_0", "name": "Trypan Blue 100ml", "x": 32, "y": 12, "w": 1, "h": 1, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Trypan_Blue_100ml.BP_Bottle_Trypan_Blue_100ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle03_100ml.png", "thumbnailImage": "containers/bottle100ml_trypan_blue.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Cryopreservation_Medium_100ml_0", "name": "Cryopreservation Medium 100ml", "x": 34, "y": 12, "w": 1, "h": 1, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Cryopresevation_Medium_100ml.BP_Bottle_Cryopresevation_Medium_100ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle03_100ml.png", "thumbnailImage": "containers/bottle100ml_cryo_medium.png", "isSurface": false, "requiresSurface": true, "items": [] } ] }, { "id": "Fridge_0", "name": "Fridge", "x": 27, "y": 26, "w": 13, "h": 14, "ueBlueprint": "/Game/Instruments/Incubators/BP_Small_Fridge_Freezer.BP_Small_Fridge_Freezer_C", "absoluteRotation": 180, "relativeRotation": 180, "gridImage": "devices/fridge_freezer_small.png", "thumbnailImage": "devices/fridge_freezer_small.png", "isSurface": false, "requiresSurface": true, "items": [] } ], "thumbnailImage": "", "gridImage": "", "parts": [] } }, { "version": "v2", "name": "Correct operation of a centrifuge", "steps": [ "The centrifuge is a device that uses centrifugal force supplied by a rotor, to subject a specimen to a high enough specified constant force to separate various components of a fluid e.g. blood cell types or cells from supernatant. It is used often prior to re-seeding cells or producing different concentrations for freezing. ", "For the purpose of this training you will learn how to dismantle, clean and reassemble the centrifuge. In addition you will learn the importance of properly balancing and running the centrifuge. ", "Press \"open\" to open the centrifuge and firstly remove the bucket caps.", "Place these to one side.", "Remove the buckets and place to one side. ", "Spray and wipe down the inside of the centrifuge and around the fixed rotor with 70% ethanol to remove any fluid stains or contaminants. ", "Dry with a paper towel", "Spray and wipe down the bucket caps and buckets (interior and exterior) with 70% ethanol and allow to airdry or dry with a paper towel. ", "Once dry, insert the buckets and caps back into the rotor, re-assembling to it's original form. These should all securely click into place. ", "Next we will practice balancing the centrifuge. ", "When running samples you should ensure that opposite buckets are balanced with the same volume of liquid and same types/sizes of tube. It is therefore important that using the serological pipette and the controller that you create pre-made volume balances. ", "For the purpose of this test trial adding both different and the same volumes of distilled water to different centrifuge tubes. ", "Then try placing them in and closing the lid to see if changing their positions affects whether the device is considered balanced. ", "Balanced centrifuge: A centrifuge is considered balanced when the tubes are arranged so that the weight is evenly distributed across the rotor. For example:\nFour tubes each containing 20 ml of liquid, placed in opposite buckets (2 opposite 2). Alternatively, six tubes with varying volumes, such as four tubes of 20 ml and two additional tubes of 10 ml each, can also be balanced if they are positioned in opposite buckets to maintain symmetry.", "Unbalanced centrifuge: A centrifuge is considered unbalanced when the tubes are not arranged symmetrically, causing uneven weight distribution. For example:\nTubes with volumes placed in adjacent buckets instead of opposite buckets. Any arrangement where the rotor experiences uneven forces e.g. opposite buckets containing different tube with different liquid volumes. ", "An unbalanced centrifuge is very dangerous and it may cause vibration, noise or potential damage to the centrifuge. If unsecured and severely unbalanced a centrifuge has the potential to move around, causing damage to the surrounding environment as well. ", "Practice different combinations until you understand the DO's and DON'T's of using a centrifuge. " ], "layout": { "id": "main", "name": "Main grid", "x": 0, "y": 0, "w": 40, "h": 40, "ueBlueprint": "", "relativeRotation": 0, "absoluteRotation": 0, "isSurface": true, "requiresSurface": false, "items": [ { "id": "Table_100_x_80_0", "name": "Table 100 x 80", "x": 0, "y": 2, "w": 20, "h": 16, "ueBlueprint": "/Game/Instruments/Tables/BP_Table_Small.BP_Table_Small_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "furniture/table_small.png", "thumbnailImage": "furniture/table_small.png", "isSurface": true, "requiresSurface": true, "items": [ { "id": "Centrifuge_Tube_50ml_0", "name": "Centrifuge Tube 50ml", "x": 10, "y": 3, "w": 4, "h": 3, "ueBlueprint": "/Game/Instruments/Centrifuge/BP_Dispenser_Centrifuge_Tube_50ml.BP_Dispenser_Centrifuge_Tube_50ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/dispenser_centrifuge_tube_50ml.png", "thumbnailImage": "containers/centrifuge_tube_50ml.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Tube_Rack_50ml_0", "name": "Tube Rack 50ml", "x": 10, "y": 7, "w": 3, "h": 1, "ueBlueprint": "/Game/Instruments/Centrifuge/TubeRacks/BP_Centrifuge_Tube_Rack_50ml.BP_Centrifuge_Tube_Rack_50ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "other/tube_rack_50ml.png", "thumbnailImage": "other/tube_rack_50ml.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Tube_Rack_50ml_1", "name": "Tube Rack 50ml", "x": 10, "y": 9, "w": 3, "h": 1, "ueBlueprint": "/Game/Instruments/Centrifuge/TubeRacks/BP_Centrifuge_Tube_Rack_50ml.BP_Centrifuge_Tube_Rack_50ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "other/tube_rack_50ml.png", "thumbnailImage": "other/tube_rack_50ml.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Tube_Rack_50ml_2", "name": "Tube Rack 50ml", "x": 14, "y": 7, "w": 3, "h": 1, "ueBlueprint": "/Game/Instruments/Centrifuge/TubeRacks/BP_Centrifuge_Tube_Rack_50ml.BP_Centrifuge_Tube_Rack_50ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "other/tube_rack_50ml.png", "thumbnailImage": "other/tube_rack_50ml.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Tube_Rack_50ml_3", "name": "Tube Rack 50ml", "x": 14, "y": 9, "w": 3, "h": 1, "ueBlueprint": "/Game/Instruments/Centrifuge/TubeRacks/BP_Centrifuge_Tube_Rack_50ml.BP_Centrifuge_Tube_Rack_50ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "other/tube_rack_50ml.png", "thumbnailImage": "other/tube_rack_50ml.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Distilled_Water_1L_0", "name": "Distilled Water 1L", "x": 15, "y": 3, "w": 3, "h": 3, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Distilled_Water_1L.BP_Bottle_Distilled_Water_1L_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle01_1l.png", "thumbnailImage": "containers/bottle1l_distilled_water.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Pipette_Controller_0", "name": "Pipette Controller", "x": 18, "y": 7, "w": 1, "h": 4, "ueBlueprint": "/Game/Instruments/Pipettes/BP_Pipette_Controller.BP_Pipette_Controller_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/pipette_controller.png", "thumbnailImage": "devices/pipette_controller.png", "isSurface": false, "requiresSurface": false, "items": [] }, { "id": "Seropipette_10ml_0", "name": "Seropipette 10ml", "x": 10, "y": 11, "w": 8, "h": 1, "ueBlueprint": "/Game/Instruments/Pipettes/Dispensers/BP_Dispenser_Seropips_10ml.BP_Dispenser_Seropips_10ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/dispenser_seropipette_10ml.png", "thumbnailImage": "containers/seropip_10ml.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Seropipette_25ml_0", "name": "Seropipette 25ml", "x": 10, "y": 13, "w": 8, "h": 1, "ueBlueprint": "/Game/Instruments/Pipettes/Dispensers/BP_Dispenser_Seropips_25ml.BP_Dispenser_Seropips_25ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/dispenser_seropipette_25ml.png", "thumbnailImage": "containers/seropip_25ml.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Centrifuge_Benchtop_Small_0", "name": "Centrifuge Benchtop Small", "x": 1, "y": 2, "w": 7, "h": 8, "ueBlueprint": "/Game/Instruments/Centrifuge/BP_Centrifuge.BP_Centrifuge_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/centrifuge_benchtop_small.png", "thumbnailImage": "devices/centrifuge_benchtop_small.png", "isSurface": false, "requiresSurface": true, "parts": [ { "slot": "MotorSnap", "ueBlueprint": "/Game/Instruments/Centrifuge/BP_Centrifuge_Rotor.BP_Centrifuge_Rotor_C", "parts": [ { "slot": "BucketSnap1", "ueBlueprint": "/Game/Instruments/Centrifuge/BP_Centrifuge_Bucket.BP_Centrifuge_Bucket_C", "parts": [ { "slot": "LidSnap", "ueBlueprint": "/Game/Instruments/Centrifuge/BP_Cap_Bucket.BP_Cap_Bucket_C", "parts": [] } ] }, { "slot": "BucketSnap2", "ueBlueprint": "/Game/Instruments/Centrifuge/BP_Centrifuge_Bucket.BP_Centrifuge_Bucket_C", "parts": [ { "slot": "LidSnap", "ueBlueprint": "/Game/Instruments/Centrifuge/BP_Cap_Bucket.BP_Cap_Bucket_C", "parts": [] } ] }, { "slot": "BucketSnap3", "ueBlueprint": "/Game/Instruments/Centrifuge/BP_Centrifuge_Bucket.BP_Centrifuge_Bucket_C", "parts": [ { "slot": "LidSnap", "ueBlueprint": "/Game/Instruments/Centrifuge/BP_Cap_Bucket.BP_Cap_Bucket_C", "parts": [] } ] }, { "slot": "BucketSnap4", "ueBlueprint": "/Game/Instruments/Centrifuge/BP_Centrifuge_Bucket.BP_Centrifuge_Bucket_C", "parts": [ { "slot": "LidSnap", "ueBlueprint": "/Game/Instruments/Centrifuge/BP_Cap_Bucket.BP_Cap_Bucket_C", "parts": [] } ] } ] } ], "items": [] } ] }, { "id": "Table_100_x_80_1", "name": "Table 100 x 80", "x": 20, "y": 2, "w": 20, "h": 16, "ueBlueprint": "/Game/Instruments/Tables/BP_Table_Small.BP_Table_Small_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "furniture/table_small.png", "thumbnailImage": "furniture/table_small.png", "isSurface": true, "requiresSurface": true, "items": [ { "id": "Ethanol_Wash_Bottle_0", "name": "Ethanol Wash Bottle", "x": 10, "y": 2, "w": 2, "h": 3, "ueBlueprint": "/Game/Instruments/Containers/SqueezeBottles/BP_Squeeze_Bottle_Ethanol.BP_Squeeze_Bottle_Ethanol_C", "absoluteRotation": 270, "relativeRotation": 270, "gridImage": "containers/wash_bottle_ethanol_5l.png", "thumbnailImage": "containers/wash_bottle_ethanol_5l.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Ethanol_Spray_Bottle_0", "name": "Ethanol Spray Bottle", "x": 9, "y": 6, "w": 3, "h": 2, "ueBlueprint": "/Game/Instruments/Sanitising/BP_Spray.BP_Spray_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "cleaning/spray_bottle.png", "thumbnailImage": "cleaning/spray_bottle.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Dispenser_Paper_Table_0", "name": "Dispenser Paper Table", "x": 15, "y": 2, "w": 4, "h": 4, "ueBlueprint": "/Game/Instruments/Sanitising/BP_Dispenser_Paper_Towel.BP_Dispenser_Paper_Towel_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "cleaning/dispenser_paper_table.png", "thumbnailImage": "cleaning/dispenser_paper_table.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Wipes_IPA_0", "name": "Wipes IPA", "x": 13, "y": 7, "w": 5, "h": 3, "ueBlueprint": "/Game/Instruments/Sanitising/BP_Dispenser_Wipes.BP_Dispenser_Wipes_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "cleaning/wipes_ipa.png", "thumbnailImage": "cleaning/wipes_ipa.png", "isSurface": false, "requiresSurface": true, "items": [] } ] }, { "id": "Bio_Bin_6L_0", "name": "Bio Bin 6L", "x": 21, "y": 18, "w": 5, "h": 4, "ueBlueprint": "/Game/Instruments/Bins/BP_Bin_6L_Bio.BP_Bin_6L_Bio_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "cleaning/bio_bin_6l.png", "thumbnailImage": "cleaning/bio_bin_6l.png", "isSurface": false, "requiresSurface": true, "items": [] } ], "thumbnailImage": "", "gridImage": "", "parts": [] } }, { "version": "v2", "name": "Performing a Cell Count (Microscope)", "steps": [ "Objective - Safely and accurately perform a manual cell count using a Haemocytometer and Digital Microscope. ", "Remove the Haemocytometer from the case. It features a small grid and a coverslip, between which cells are introduced and contained for counting.", "Remove a vial of cells from the dispenser ready for counting. Place it on the surface and open it by tapping the lid.", "Retrieve a microcentrifuge tube from the dispenser box. ", "Place it in the rack and pop it open.", "Retrieve a 1000ul micropipette from the pipette rack and pop a tip on the end by inserting it into the colour matched tip box.", "Insert tip into the vial of cells and using the correct pipetting action (demonstrated by the trainer*), mix up and down. *This action is, thumb up to withdraw and thumb down to expel. ", "Cell mixing is important as they are not dissolved within the liquid and can settle at the bottom due to gravity. Mixing evenly distributes them and means that a representative sample can be taken. ", "Once mixed and the tip empty, hover the tip over the sharps box to remove it. The sharps box is the yellow bin on the table. ", "Return the 1000ul pipette to the pipette stand. ", "Remove a 10ul micropipette from the pipette rack and pop a tip on the end by stabbing at the colour matched tip box.", "Insert 10ul pipette tip into vial of cells suspension and withdraw 10ul.", "Once full, place the tip inside the microcentrifuge tube and expel the 10ul cell suspension.", "Discard of the empty tip by hovering the micropipette over and inserting the tip into the sharps bin.", "Tap the lid of the Trypan Blue bottle to open it.", "Pop a fresh tip back on the end of the 10ul micropipette by stabbing at the colour matched tip box.", "Insert 10ul pipette tip into Trypan Blue and withdraw 10ul.", "Once full, place the tip inside the microcentrifuge and expel the 10ul Trypan Blue.", "Mix the cell and Trypan Blue suspension up and down with the same pipette and then withdraw 10ul to add to the Haemocytometer.", "Load 10μl onto the Haemocytometer where the coverslip meets the counting area – the sample will be drawn underneath by capillary action.", "Dispose of the used pipette tip into the sharps bin. Close all the lids on bottles and tubes and place the micropipette back onto the pipette rack. ", "Place the assembled Haemocytometer into the slide retainer on the Microscope.", "On a real laboratory Microscope you would have it set to 10x objective (yellow ring lense) and a brightness setting of 3. ", "On a real laboratory Microscope, you would be able to navigate to the counting grid by using the repositioning knobs. This VR Microscope allows you to focus on the haemocytometer grid using the knobs on each side but the position is pre-set so the entire counting grid is visible. ", "If the microscope fails to focus on the grid, then try removing the haemocytometer and re-placing it down.", "Count at least the top left and bottom right large squares using a tally counter. Ideally you should count all four corner squares and central square in the chamber. ", "Treat cells that are more than 50% within the outer lines of the main square as countable. Count in a snaking pattern left to right within each large square, ensuring you move through all the small squares within the large square.", "In a real lab environment you would make a note of your counts and create an average of all large squares counted e.g. 3 squares = 30 cells, 30/3 = 10 cells per square. For the purpose of VR, make a mental note of your average. This is the basis for how a cell count is performed. The next steps involve finding out how many cells were in the initial vial we took the sample from. To do this:", "Multiply the result by 10,000 to convert to cells/ml in Trypan Blue", "Multiply your answer by 2 to determine the original cell concentration in the cell suspension vial (diluted 2x in Typan Blue for this protocol). ", "You have successfully performed a manual cell count on a Haemocytometer. " ], "layout": { "id": "main", "name": "Main grid", "x": 0, "y": 0, "w": 40, "h": 40, "ueBlueprint": "", "relativeRotation": 0, "absoluteRotation": 0, "isSurface": true, "requiresSurface": false, "items": [ { "id": "Table_with_Hazard_Cabinets_0", "name": "Table with Hazard Cabinets", "x": 0, "y": 7, "w": 40, "h": 15, "ueBlueprint": "/Game/Instruments/Tables/BP_Cabinet_Tabletop.BP_Cabinet_Tabletop_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "furniture/cabinets_with_table.png", "thumbnailImage": "furniture/cabinets_with_table.png", "isSurface": true, "requiresSurface": true, "items": [ { "id": "Pipettes_Mechanical_10ul,_25ul,_100ul,_1000ul_0", "name": "Pipettes Mechanical 10ul, 25ul, 100ul, 1000ul", "x": 17, "y": 3, "w": 5, "h": 3, "ueBlueprint": "/Game/Instruments/Pipettes/BP_Mechanical_Pipette_Stand.BP_Mechanical_Pipette_Stand_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/mechanical_pipette_rack.png", "thumbnailImage": "devices/mechanical_pipette_rack.png", "isSurface": false, "requiresSurface": true, "parts": [ { "slot": "Pipette10Snap", "ueBlueprint": "/Game/Instruments/Pipettes/BP_Mechanical_Pipette_Controller_10.BP_Mechanical_Pipette_Controller_10_C" }, { "slot": "Pipette20Snap", "ueBlueprint": "/Game/Instruments/Pipettes/BP_Mechanical_Pipette_Controller_20.BP_Mechanical_Pipette_Controller_20_C" }, { "slot": "Pipette200Snap", "ueBlueprint": "/Game/Instruments/Pipettes/BP_Mechanical_Pipette_Controller_200.BP_Mechanical_Pipette_Controller_200_C" }, { "slot": "Pipette1000Snap", "ueBlueprint": "/Game/Instruments/Pipettes/BP_Mechanical_Pipette_Controller_1000.BP_Mechanical_Pipette_Controller_1000_C" } ], "items": [] }, { "id": "Pipette_tip_box_20ul_0", "name": "Pipette tip box 20ul", "x": 24, "y": 3, "w": 3, "h": 3, "ueBlueprint": "/Game/Instruments/Pipettes/Dispensers/BP_Dispenser_Micro_Pipettes_20.BP_Dispenser_Micro_Pipettes_20_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/dispenser_pipette_tips_20ul.png", "thumbnailImage": "containers/dispenser_pipette_tips_20ul.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Pipette_tip_box_10ul_0", "name": "Pipette tip box 10ul", "x": 27, "y": 3, "w": 3, "h": 3, "ueBlueprint": "/Game/Instruments/Pipettes/Dispensers/BP_Dispenser_Micro_Pipettes_10.BP_Dispenser_Micro_Pipettes_10_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/dispenser_pipette_tips_10ul.png", "thumbnailImage": "containers/dispenser_pipette_tips_10ul.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Vial_of_Cells_0", "name": "Vial of Cells", "x": 29, "y": 8, "w": 4, "h": 3, "ueBlueprint": "/Game/Instruments/Containers/Dispensers/BP_Dispenser_Vial_Of_Cells.BP_Dispenser_Vial_Of_Cells_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/dispenser_vial_of_cells.png", "thumbnailImage": "containers/vial_of_cells.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Haemocytometer_0", "name": "Haemocytometer", "x": 24, "y": 8, "w": 3, "h": 3, "ueBlueprint": "/Game/Instruments/MicroScope/BP_Haemocytometer_Case.BP_Haemocytometer_Case_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/haemocytometer.png", "thumbnailImage": "devices/haemocytometer.png", "isSurface": false, "requiresSurface": true, "parts": [ { "slot": "HmSnap", "ueBlueprint": "/Game/Instruments/MicroScope/BP_Haemocytometer.BP_Haemocytometer_C", "parts": [] } ], "items": [] }, { "id": "Tube_Rack_Micro_0", "name": "Tube Rack Micro", "x": 19, "y": 10, "w": 3, "h": 1, "ueBlueprint": "/Game/Instruments/Centrifuge/TubeRacks/BP_Centrifuge_Tube_Rack_Micro.BP_Centrifuge_Tube_Rack_Micro_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "other/tube_rack_micro.png", "thumbnailImage": "other/tube_rack_micro.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Microcentrifuge_Tube_1.5ml_0", "name": "Microcentrifuge Tube 1.5ml", "x": 19, "y": 7, "w": 3, "h": 2, "ueBlueprint": "/Game/Instruments/Centrifuge/BP_Dispenser_Microcentrifuge_Tube_1halfml.BP_Dispenser_Microcentrifuge_Tube_1halfml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/dispenser_microcentrifuge_tube_one_half_ml.png", "thumbnailImage": "containers/microcentrifuge_tube_one_half_ml.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Ethanol_Spray_Bottle_0", "name": "Ethanol Spray Bottle", "x": 2, "y": 4, "w": 3, "h": 2, "ueBlueprint": "/Game/Instruments/Sanitising/BP_Spray.BP_Spray_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "cleaning/spray_bottle.png", "thumbnailImage": "cleaning/spray_bottle.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Ethanol_Wash_Bottle_0", "name": "Ethanol Wash Bottle", "x": 2, "y": 7, "w": 3, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/SqueezeBottles/BP_Squeeze_Bottle_Ethanol.BP_Squeeze_Bottle_Ethanol_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/wash_bottle_ethanol_5l.png", "thumbnailImage": "containers/wash_bottle_ethanol_5l.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Wipes_IPA_0", "name": "Wipes IPA", "x": 1, "y": 10, "w": 5, "h": 3, "ueBlueprint": "/Game/Instruments/Sanitising/BP_Dispenser_Wipes.BP_Dispenser_Wipes_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "cleaning/wipes_ipa.png", "thumbnailImage": "cleaning/wipes_ipa.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Microscope_Digital_0", "name": "Microscope Digital", "x": 7, "y": 3, "w": 9, "h": 7, "ueBlueprint": "/Game/Instruments/MicroScope/BP_Digital_Microscope.BP_Digital_Microscope_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/microscope_digital.png", "thumbnailImage": "devices/microscope_digital.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Trypan_Blue_100ml_0", "name": "Trypan Blue 100ml", "x": 16, "y": 9, "w": 1, "h": 1, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Trypan_Blue_100ml.BP_Bottle_Trypan_Blue_100ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle03_100ml.png", "thumbnailImage": "containers/bottle100ml_trypan_blue.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Pipette_tip_box_1000ul_0", "name": "Pipette tip box 1000ul", "x": 30, "y": 3, "w": 3, "h": 3, "ueBlueprint": "/Game/Instruments/Pipettes/Dispensers/BP_Dispenser_Micro_Pipettes_1000.BP_Dispenser_Micro_Pipettes_1000_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/dispenser_pipette_tips_1000ul.png", "thumbnailImage": "containers/dispenser_pipette_tips_1000ul.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Bin_Sharps_0", "name": "Bin Sharps", "x": 34, "y": 8, "w": 4, "h": 4, "ueBlueprint": "/Game/Instruments/Bins/BP_Bin_Sharps.BP_Bin_Sharps_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "cleaning/bin_sharps.png", "thumbnailImage": "cleaning/bin_sharps.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Pipette_tip_box_200ul_0", "name": "Pipette tip box 200ul", "x": 33, "y": 3, "w": 3, "h": 3, "ueBlueprint": "/Game/Instruments/Pipettes/Dispensers/BP_Dispenser_Micro_Pipettes_200.BP_Dispenser_Micro_Pipettes_200_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/dispenser_pipette_tips_200ul.png", "thumbnailImage": "containers/dispenser_pipette_tips_200ul.png", "isSurface": false, "requiresSurface": true, "items": [] } ] }, { "id": "Bio_Bin_6L_0", "name": "Bio Bin 6L", "x": 35, "y": 23, "w": 5, "h": 4, "ueBlueprint": "/Game/Instruments/Bins/BP_Bin_6L_Bio.BP_Bin_6L_Bio_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "cleaning/bio_bin_6l.png", "thumbnailImage": "cleaning/bio_bin_6l.png", "isSurface": false, "requiresSurface": true, "items": [] } ], "thumbnailImage": "", "gridImage": "", "parts": [] } }, { "version": "v2", "name": "Creating PBS", "steps": [ "The objective of this protocol is to learn how to create Phosphate Buffered Saline (PBS). This buffer is one of the most commonly used in Life Science laboratories.", "Turn on the fume hood and airflow. ", "Measure out 790ml of distilled water in a suitable container using a serological pipette. It is recommended you use a 1L bottle suitable for autoclaving.", "Discard of the used serological pipette and move the bottle slightly to one side. ", "Grab a funnel from the dispenser and place inside the bottle.", "Leave this here ready to add each powder one by one to the bottle.", "Turn on the analytical scale and check it is properly balanced.", "Grab the small weigh boat from the dispenser. ", "Place the weigh boat on the analytical scale and tare with the door closed", "Selecting the correct measuring spatula, add 4g of Sodium Chloride powder total to the weigh boat bit by bit*. Tilt the spatula only slightly and let the powder fall in a controlled trickle to release less or tilt at a steeper angle to release more. ", "*The spatula will automatically take it's max capacity every time you pick up or remove the powder so be aware of this when trying to dispense an exact amount onto the weigh boat. ", "Once you have measured 4g Sodium Chloride into the weigh boat, poor the weigh boat contents into the funnel. Be careful not to spill any contents outside of the funnel. ", "Repeat this again, making sure you re-tare between measurements, so that in total you can added 8g Sodium Chloride into the solution", "Rinse the spatula off into the beaker and dry to remove contaminants. ", "Discard of this weigh boat and retrieve another. ", "Re-tare the scale with the weigh boat.", "Measure out 0.2g Potassium Chloride powder and add to the solution through the funnel.", "Repeat steps 12-14 each time between chemicals. ", "Measure out 1.44g of Sodium Phosphate Dibasic and add to the solution through the funnel. ", "Repeat steps 12-14 ", "Measure out 0.245g of Potassium Phosphate Monobasic and add to the solution through the funnel. ", "Discard of the weigh boat and rinse/dry the spatula. ", "Using a serological pipette, add the remaining 10ml distilled water to the funnel to swill all the remaining powder into the bottle (now 800ml total volume distilled water).", "Carefully remove the funnel from the bottle and replace the bottle cap. ", "In a real lab you would place the funnel into a designated area for dirty glassware/plasticware prior to cleaning. For the purpose of this virtual protocol, place the funnel in the bin as we have created all the funnels to be single-use. ", "Ensuring the bottle is securely capped and sealed, place it on the orbital shaker to give the soon to be buffer a good mix*. ", "*In a real world setting you would want to make sure that you label up the bottle with it's current ingredients and your initials so that other lab personnel are aware of what is in the bottle. ", "Once the bottle has been shaking for 15 mins or more and is well mixed, move it off the orbital shaker and onto the work surface.", "Move to the fume hood and get it set up so that the PH of the buffer can be adjusted using HCl. ", "Place the bottle of buffer into the fume hood and turn on the PH meter.", "Perform a quick calibration of the PH meter if necessary, ensuring you wash the PH meter with distilled water prior to measuring the PH of the buffer. ", "Place the bottle of buffer under the PH meter to perform a measurement.", "Depending on what the PH falls as, use the HCl to adjust the solution to the desired PH of PH7*. ", "*Add the HCl slowly using the smallest micropipette initially. Depending on how much it appears that you need to add, continue adding using a larger volume micropipette and adding HCl bit by bit. " ], "layout": { "id": "main", "name": "Main grid", "x": 0, "y": 0, "w": 40, "h": 40, "ueBlueprint": "", "relativeRotation": 0, "absoluteRotation": 0, "isSurface": true, "requiresSurface": false, "items": [ { "id": "Table_200_x_70_0", "name": "Table 200 x 70", "x": 0, "y": 26, "w": 40, "h": 14, "ueBlueprint": "/Game/Instruments/Tables/BP_Table_200x70.BP_Table_200x70_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "furniture/table_200x70.png", "thumbnailImage": "furniture/table_large.png", "isSurface": true, "requiresSurface": true, "items": [ { "id": "Orbital_Shaker_0", "name": "Orbital Shaker", "x": 31, "y": 5, "w": 8, "h": 9, "ueBlueprint": "/Game/Instruments/OrbitalShaker/BP_Orbital_Shaker.BP_Orbital_Shaker_C", "absoluteRotation": 180, "relativeRotation": 180, "gridImage": "devices/orbital_shaker.png", "thumbnailImage": "devices/orbital_shaker.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Seropipette_100ml_0", "name": "Seropipette 100ml", "x": 31, "y": 1, "w": 8, "h": 2, "ueBlueprint": "/Game/Instruments/Pipettes/Dispensers/BP_Dispenser_Seropips_100ml.BP_Dispenser_Seropips_100ml_C", "absoluteRotation": 180, "relativeRotation": 180, "gridImage": "containers/dispenser_seropipette_100ml.png", "thumbnailImage": "containers/seropip_100ml.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Seropipette_10ml_0", "name": "Seropipette 10ml", "x": 31, "y": 3, "w": 8, "h": 1, "ueBlueprint": "/Game/Instruments/Pipettes/Dispensers/BP_Dispenser_Seropips_10ml.BP_Dispenser_Seropips_10ml_C", "absoluteRotation": 180, "relativeRotation": 180, "gridImage": "containers/dispenser_seropipette_10ml.png", "thumbnailImage": "containers/seropip_10ml.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Spatula_Case_0", "name": "Spatula Case", "x": 25, "y": 7, "w": 3, "h": 6, "ueBlueprint": "/Game/Instruments/Spatula/BP_Spatula_Set.BP_Spatula_Set_C", "absoluteRotation": 180, "relativeRotation": 180, "gridImage": "containers/spatula_case.png", "thumbnailImage": "containers/spatula_case.png", "isSurface": false, "requiresSurface": true, "parts": [ { "slot": "Snap1", "ueBlueprint": "/Game/Instruments/Spatula/BP_Spatula_1g.BP_Spatula_1g_C", "parts": [] }, { "slot": "Snap10", "ueBlueprint": "/Game/Instruments/Spatula/BP_Spatula_10g.BP_Spatula_10g_C", "parts": [] }, { "slot": "Snap50", "ueBlueprint": "/Game/Instruments/Spatula/BP_Spatula_50g.BP_Spatula_50g_C", "parts": [] } ], "items": [] }, { "id": "Pipette_Controller_0", "name": "Pipette Controller", "x": 29, "y": 9, "w": 1, "h": 4, "ueBlueprint": "/Game/Instruments/Pipettes/BP_Pipette_Controller.BP_Pipette_Controller_C", "absoluteRotation": 180, "relativeRotation": 180, "gridImage": "devices/pipette_controller.png", "thumbnailImage": "devices/pipette_controller.png", "isSurface": false, "requiresSurface": false, "items": [] }, { "id": "Funnel_254ml_0", "name": "Funnel 254ml", "x": 26, "y": 3, "w": 4, "h": 3, "ueBlueprint": "/Game/Instruments/Funnel/BP_Dispenser_Funnel_254ml.BP_Dispenser_Funnel_254ml_C", "absoluteRotation": 180, "relativeRotation": 180, "gridImage": "containers/funnel_254ml.png", "thumbnailImage": "containers/funnel_254ml.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Potassium_Chloride_Powder_100g_0", "name": "Potassium Chloride Powder 100g", "x": 23, "y": 1, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Powders/BP_Potassium_Chloride_100g.BP_Potassium_Chloride_100g_C", "absoluteRotation": 180, "relativeRotation": 180, "gridImage": "containers/bottle02_100ml.png", "thumbnailImage": "containers/potassium_chloride_powder_100g.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Sodium_Phosphate_Dibasic_100g_0", "name": "Sodium Phosphate Dibasic 100g", "x": 21, "y": 1, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Powders/BP_Sodium_Phosphate_Dibasic_100g.BP_Sodium_Phosphate_Dibasic_100g_C", "absoluteRotation": 180, "relativeRotation": 180, "gridImage": "containers/bottle02_100ml.png", "thumbnailImage": "containers/sodium_phospate_dibasic_powder_100g.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Sodium_Chloride_Powder_100g_0", "name": "Sodium Chloride Powder 100g", "x": 21, "y": 3, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Powders/BP_Sodium_Chloride_100g.BP_Sodium_Chloride_100g_C", "absoluteRotation": 180, "relativeRotation": 180, "gridImage": "containers/bottle02_100ml.png", "thumbnailImage": "containers/sodium_chloride_powder_100g.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Potassium_Phosphate_Monobasic_100g_0", "name": "Potassium Phosphate Monobasic 100g", "x": 23, "y": 3, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Powders/BP_Potassium_Phosphate_Monobasic_100g.BP_Potassium_Phosphate_Monobasic_100g_C", "absoluteRotation": 180, "relativeRotation": 180, "gridImage": "containers/bottle02_100ml.png", "thumbnailImage": "containers/potassium_phosphate_monobasic_100g.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Analytical_Scale_0", "name": "Analytical Scale", "x": 2, "y": 6, "w": 7, "h": 7, "ueBlueprint": "/Game/Instruments/Measuring/WeighingScale/BP_Analytical_Scale.BP_Analytical_Scale_C", "absoluteRotation": 180, "relativeRotation": 180, "gridImage": "devices/analytical_scale.png", "thumbnailImage": "devices/analytical_scale.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Analytical_Scale_-_Calibration_Weights_0", "name": "Analytical Scale - Calibration Weights", "x": 10, "y": 10, "w": 6, "h": 2, "ueBlueprint": "/Game/Instruments/Measuring/WeighingScale/CalibrationWeights/BP_Calibration_Weights_Box.BP_Calibration_Weights_Box_C", "absoluteRotation": 180, "relativeRotation": 180, "gridImage": "devices/analytical_scale_weights.png", "thumbnailImage": "devices/analytical_scale_weights.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Bottle_1L_0", "name": "Bottle 1L", "x": 11, "y": 3, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/GL45/BP_Bottle_GL45_1L.BP_Bottle_GL45_1L_C", "absoluteRotation": 180, "relativeRotation": 180, "gridImage": "containers/bottle03_1l.png", "thumbnailImage": "containers/bottle03_1l.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Distilled_Water_1L_0", "name": "Distilled Water 1L", "x": 17, "y": 10, "w": 3, "h": 3, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Distilled_Water_1L.BP_Bottle_Distilled_Water_1L_C", "absoluteRotation": 180, "relativeRotation": 180, "gridImage": "containers/bottle01_1l.png", "thumbnailImage": "containers/bottle1l_distilled_water.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Funnel_64ml_0", "name": "Funnel 64ml", "x": 22, "y": 11, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Funnel/BP_Dispenser_Funnel_64ml.BP_Dispenser_Funnel_64ml_C", "absoluteRotation": 180, "relativeRotation": 180, "gridImage": "containers/funnel_64ml.png", "thumbnailImage": "containers/funnel_64ml.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Weigh_Boat_7g_0", "name": "Weigh Boat 7g", "x": 22, "y": 8, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/WeighingBoats/BP_Dispenser_Weighing_Boat_7ml.BP_Dispenser_Weighing_Boat_7ml_C", "absoluteRotation": 180, "relativeRotation": 180, "gridImage": "containers/weigh_boat_7ml.png", "thumbnailImage": "containers/weigh_boat_7ml.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Beaker_250ml_1", "name": "Beaker 250ml", "x": 18, "y": 7, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Beakers/BP_Beaker_250ml.BP_Beaker_250ml_C", "absoluteRotation": 90, "relativeRotation": 90, "gridImage": "containers/beaker_250ml.png", "thumbnailImage": "containers/beaker_250ml.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Ethanol_Wash_Bottle_0", "name": "Ethanol Wash Bottle", "x": 17, "y": 5, "w": 3, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/SqueezeBottles/BP_Squeeze_Bottle_Ethanol.BP_Squeeze_Bottle_Ethanol_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/wash_bottle_ethanol_5l.png", "thumbnailImage": "containers/wash_bottle_ethanol_5l.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Water_Wash_Bottle_0", "name": "Water Wash Bottle", "x": 17, "y": 3, "w": 3, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/SqueezeBottles/BP_Squeeze_Bottle_Water.BP_Squeeze_Bottle_Water_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/wash_bottle_water_5l.png", "thumbnailImage": "containers/wash_bottle_water_5l.png", "isSurface": false, "requiresSurface": true, "items": [] } ] }, { "id": "Table_60_x_45_0", "name": "Table 60 x 45", "x": 0, "y": 0, "w": 12, "h": 9, "ueBlueprint": "/Game/Instruments/Tables/BP_Table_Stand_Small.BP_Table_Stand_Small_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "furniture/table_stand.png", "thumbnailImage": "furniture/table_stand.png", "isSurface": true, "requiresSurface": true, "items": [ { "id": "Corrosives_Cabinet_0", "name": "Corrosives Cabinet", "x": 3, "y": 1, "w": 8, "h": 6, "ueBlueprint": "/Game/Instruments/HazardsCabinets/BP_Hazards_Cabinet_Corrosive.BP_Hazards_Cabinet_Corrosive_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "furniture/cabinet_small.png", "thumbnailImage": "furniture/cabinet_corrosives.png", "isSurface": false, "requiresSurface": true, "items": [] } ] }, { "id": "Fume_Cabinet_0", "name": "Fume Cabinet", "x": 12, "y": 0, "w": 28, "h": 13, "ueBlueprint": "/Game/Instruments/FumeHood/BP_Fume_Hood.BP_Fume_Hood_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/fume_hood.png", "thumbnailImage": "devices/fume_hood.png", "isSurface": true, "requiresSurface": false, "items": [ { "id": "Pipettes_Mechanical_10ul,_25ul,_100ul,_1000ul_0", "name": "Pipettes Mechanical 10ul, 25ul, 100ul, 1000ul", "x": 21, "y": 2, "w": 5, "h": 3, "ueBlueprint": "/Game/Instruments/Pipettes/BP_Mechanical_Pipette_Stand.BP_Mechanical_Pipette_Stand_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/mechanical_pipette_rack.png", "thumbnailImage": "devices/mechanical_pipette_rack.png", "isSurface": false, "requiresSurface": true, "parts": [ { "slot": "Pipette10Snap", "ueBlueprint": "/Game/Instruments/Pipettes/BP_Mechanical_Pipette_Controller_10.BP_Mechanical_Pipette_Controller_10_C" }, { "slot": "Pipette20Snap", "ueBlueprint": "/Game/Instruments/Pipettes/BP_Mechanical_Pipette_Controller_20.BP_Mechanical_Pipette_Controller_20_C" }, { "slot": "Pipette200Snap", "ueBlueprint": "/Game/Instruments/Pipettes/BP_Mechanical_Pipette_Controller_200.BP_Mechanical_Pipette_Controller_200_C" }, { "slot": "Pipette1000Snap", "ueBlueprint": "/Game/Instruments/Pipettes/BP_Mechanical_Pipette_Controller_1000.BP_Mechanical_Pipette_Controller_1000_C" } ], "items": [] }, { "id": "Hydrochloric_Acid_1L_0", "name": "Hydrochloric Acid 1L", "x": 24, "y": 6, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Hydrochloric_Acid_1L.BP_Bottle_Hydrochloric_Acid_1L_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle06_1l.png", "thumbnailImage": "containers/bottle1l_hydrochloric_acid.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Pipette_tip_box_1000ul_0", "name": "Pipette tip box 1000ul", "x": 17, "y": 2, "w": 3, "h": 3, "ueBlueprint": "/Game/Instruments/Pipettes/Dispensers/BP_Dispenser_Micro_Pipettes_1000.BP_Dispenser_Micro_Pipettes_1000_C", "absoluteRotation": 180, "relativeRotation": 180, "gridImage": "containers/dispenser_pipette_tips_1000ul.png", "thumbnailImage": "containers/dispenser_pipette_tips_1000ul.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Pipette_tip_box_20ul_0", "name": "Pipette tip box 20ul", "x": 13, "y": 2, "w": 3, "h": 3, "ueBlueprint": "/Game/Instruments/Pipettes/Dispensers/BP_Dispenser_Micro_Pipettes_20.BP_Dispenser_Micro_Pipettes_20_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/dispenser_pipette_tips_20ul.png", "thumbnailImage": "containers/dispenser_pipette_tips_20ul.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Ph_Meter_0", "name": "Ph Meter", "x": 2, "y": 1, "w": 7, "h": 5, "ueBlueprint": "/Game/Instruments/Measuring/pHReader/BP_pH_Reader.BP_pH_Reader_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/ph_meter.png", "thumbnailImage": "devices/ph_meter.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "pH10_solution_100ml_0", "name": "pH10 solution 100ml", "x": 22, "y": 6, "w": 1, "h": 1, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_pH_10_Solution.BP_Bottle_pH_10_Solution_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle03_100ml.png", "thumbnailImage": "containers/bottle100ml_ph10.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "pH4_solution_100ml_0", "name": "pH4 solution 100ml", "x": 20, "y": 6, "w": 1, "h": 1, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_pH_4_Solution.BP_Bottle_pH_4_Solution_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle03_100ml.png", "thumbnailImage": "containers/bottle100ml_ph4.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "pH7_solution_100ml_0", "name": "pH7 solution 100ml", "x": 18, "y": 6, "w": 1, "h": 1, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_pH_7_Solution.BP_Bottle_pH_7_Solution_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle03_100ml.png", "thumbnailImage": "containers/bottle100ml_ph7.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Beaker_250ml_0", "name": "Beaker 250ml", "x": 2, "y": 7, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Beakers/BP_Beaker_250ml.BP_Beaker_250ml_C", "absoluteRotation": 270, "relativeRotation": 270, "gridImage": "containers/beaker_250ml.png", "thumbnailImage": "containers/beaker_250ml.png", "isSurface": false, "requiresSurface": true, "items": [] } ] }, { "id": "Bio_Bin_6L_0", "name": "Bio Bin 6L", "x": 0, "y": 9, "w": 5, "h": 4, "ueBlueprint": "/Game/Instruments/Bins/BP_Bin_6L_Bio.BP_Bin_6L_Bio_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "cleaning/bio_bin_6l.png", "thumbnailImage": "cleaning/bio_bin_6l.png", "isSurface": false, "requiresSurface": true, "items": [] } ], "thumbnailImage": "", "gridImage": "", "parts": [] } }, { "name": "Weighing solids", "steps": [ "Purpose and Scope: Training for safe and effective weighing of solids/powders.", "When weighing any hazardous chemicals or powders it is important to consider doing so in the fume hood. ", "Firstly, turn the fume hood on, turn the airflow and light on. Ensure the sash is at a safe, workable height. ", "Check for visible damage, loose components, or debris on the weighing platform.", "Ensure the draft shield door on the scales is closed. ", "Turn on the analytical scale and allow it to stabilise.", "Check the scale is balanced by inspecting the indicator. The bubble should be in the centre of the indicator ring. If not this can be adjusted by tapping each wheel. ", "Check that the measurement mode is in grams.", "For routine use, you should ideally calibrate the analytical scale weekly but for high accuracy applications, you should do so before each use or daily. For this protocol the calibration steps are optional so feel free to skip to step 20.", "Press the Function then Tare button to access to the calibration screen.", "Gently open the draft shield door.", "Pick up the smallest/lightest calibration weight from the box outside the fume hood and place in the centre of the scale. ", "Gently close the draft shield door by touching the handle.", "The scale should show weight but wait a few seconds until balance settles on a weight.", "Make a note of the weight in a calibration book.", "Gently open the draft shield door by touching the handle.", "Remove the weight from the scale and close the draft shield door.", "Re-TARE the scale.", "Repeat the calibration/calibration weight weighing process with the other weights in order lightest-heaviest.", "Once all the weights have been calibrated , double tap the function button to take you back to the sample measuring mode.", "Press the TARE button. ", "The purpose of the remainder of this protocol is to illustrate the proper practice for weighing solids and powders. Technique is usually dependent on the type and volume of solid you are weighing and apparatus used. ", "Firstly we will attempt to weigh 5g powder into a beaker. ", "Gently open the draft shield door. ", "Place the smallest weigh boat on the scale and press TARE.", "Selecting the most applicable spatula, measure 5g of any powder into the weigh boat. You may choose to use a smaller spatula multiple times or a larger spatula but very slowly dispense the powder into the weight boat. ", "Tilt the spatula only slightly and let the powder fall in a controlled trickle to release less or tilt at a steeper angle to release more*. *The spatula will automatically take it's max capacity every time you pick up or remove the powder so be aware of this when trying to dispense an exact amount onto the weigh boat. ", "Once you have 5g, shut the glass shield door to ensure it settles on as close to 5g as possible. ", "Then pick up the weigh boat and tilt gently above the glass beaker to transfer 5g. ", "Dispose of the weigh boat once empty and rinse off the spatula using the water wash bottle into a spare beaker to the far side of the fume hood. ", "Repeat steps 23-28 but now using a larger capacity weigh boat and spatula to transfer 50g of the same powder to the same beaker. ", "For small quantities, use the smallest weigh boat and pour into an open beaker. For larger quantities in larger weigh boats, select a container that can hold a funnel and direct the powder into the funnel cone. This larger target area reduces spillage and is especially helpful when you’re new to the technique.", "Test weighing different amounts of different powders into different types of containers. See how accurately you can weigh out specific amounts, such as 10.55g. or any container with a neck narrower than a beaker, insert an appropriately sized funnel to ensure safe, accurate transfer.", "Avoid using a funnel for tiny amounts—significant losses can occur. Instead, choose the smallest weigh boat possible and transfer into a container with a suitably wide opening. If this feels fiddly, practise first with a low-importance solid of similar texture. If the final container is small and lightweight (e.g., a plastic tube), you may tare the analytical balance with the empty vessel and transfer directly with a spatula; take care not to overshoot, as removing excess from small vessels can be difficult.", "Before pouring, it is also important to plan how you will clear any residual powder from the funnel.", "Remove residual powder by gently tapping the funnel against the container neck to shake fragments through. If preparing a solution, rinse the funnel with the remaining liquid (e.g., the buffer) to wash particulates through.", "When finished, clean or dispose of chemicals and apparatus as appropriate, wipe down all surfaces, and shut down the analytical balance and any other devices properly." ], "layout": { "id": "main", "name": "Main grid", "x": 0, "y": 0, "w": 40, "h": 40, "ueBlueprint": "", "relativeRotation": 0, "absoluteRotation": 0, "isSurface": true, "requiresSurface": false, "items": [ { "id": "Fume_Cabinet_0", "name": "Fume Cabinet", "x": 0, "y": 0, "w": 28, "h": 13, "ueBlueprint": "/Game/Instruments/FumeHood/BP_Fume_Hood.BP_Fume_Hood_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/fume_hood.png", "thumbnailImage": "devices/fume_hood.png", "isSurface": true, "requiresSurface": false, "items": [ { "id": "Analytical_Scale_0", "name": "Analytical Scale", "x": 2, "y": 1, "w": 7, "h": 7, "ueBlueprint": "/Game/Instruments/Measuring/WeighingScale/BP_Analytical_Scale.BP_Analytical_Scale_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/analytical_scale.png", "thumbnailImage": "devices/analytical_scale.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Bin_Sharps_0", "name": "Bin Sharps", "x": 22, "y": 1, "w": 4, "h": 4, "ueBlueprint": "/Game/Instruments/Bins/BP_Bin_Sharps.BP_Bin_Sharps_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "cleaning/bin_sharps.png", "thumbnailImage": "cleaning/bin_sharps.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Bio_Bin_1L_0", "name": "Bio Bin 1L", "x": 17, "y": 1, "w": 5, "h": 4, "ueBlueprint": "/Game/Instruments/Bins/BP_Bin_1L_Bio.BP_Bin_1L_Bio_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "cleaning/bio_bin_1l.png", "thumbnailImage": "cleaning/bio_bin_1l.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Beaker_250ml_0", "name": "Beaker 250ml", "x": 14, "y": 2, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Beakers/BP_Beaker_250ml.BP_Beaker_250ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/beaker_250ml.png", "thumbnailImage": "containers/beaker_250ml.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Potassium_Nitrate_Powder_100g_0", "name": "Potassium Nitrate Powder 100g", "x": 20, "y": 5, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Powders/BP_Potassium_Nitrate_100g.BP_Potassium_Nitrate_100g_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle02_100ml.png", "thumbnailImage": "containers/potassium_nitrate_powder_100g.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Tromethamine_Powder_100g_0", "name": "Tromethamine Powder 100g", "x": 20, "y": 9, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Powders/BP_Tromethamine_100g.BP_Tromethamine_100g_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle02_100ml.png", "thumbnailImage": "containers/tromethamine_powder_100g.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Tube_Rack_50ml_0", "name": "Tube Rack 50ml", "x": 10, "y": 2, "w": 3, "h": 1, "ueBlueprint": "/Game/Instruments/Centrifuge/TubeRacks/BP_Centrifuge_Tube_Rack_50ml.BP_Centrifuge_Tube_Rack_50ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "other/tube_rack_50ml.png", "thumbnailImage": "other/tube_rack_50ml.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Volumetric_Flask_100ml_0", "name": "Volumetric Flask 100ml", "x": 10, "y": 4, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Flask/BP_Volumetric_Flask_100ml.BP_Volumetric_Flask_100ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/volumetric_flask_100ml.png", "thumbnailImage": "containers/volumetric_flask.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Bottle_250ml_0", "name": "Bottle 250ml", "x": 13, "y": 5, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/GL45/BP_Bottle_GL45_250ml.BP_Bottle_GL45_250ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle02_250ml.png", "thumbnailImage": "containers/bottle02_250ml.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Bottle_100ml_0", "name": "Bottle 100ml", "x": 11, "y": 7, "w": 1, "h": 1, "ueBlueprint": "/Game/Instruments/Containers/GL45/BP_Bottle_GL45_100ml.BP_Bottle_GL45_100ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle05_100ml.png", "thumbnailImage": "containers/bottle05_100ml.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Beaker_600ml_0", "name": "Beaker 600ml", "x": 23, "y": 6, "w": 3, "h": 3, "ueBlueprint": "/Game/Instruments/Containers/Beakers/BP_Beaker_600ml.BP_Beaker_600ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/beaker_600ml.png", "thumbnailImage": "containers/beaker_600ml.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "HEPES_Powder_100g_0", "name": "HEPES Powder 100g", "x": 24, "y": 9, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Powders/BP_HEPES_100g.BP_HEPES_100g_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle02_100ml.png", "thumbnailImage": "containers/hepes_powder_100g.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Sodium_Hydroxide_Powder_100g_0", "name": "Sodium Hydroxide Powder 100g", "x": 22, "y": 9, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Powders/BP_Sodium_Hydroxide_100g.BP_Sodium_Hydroxide_100g_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle02_100ml.png", "thumbnailImage": "containers/sodium_hydroxide_powder_100g.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Sodium_Chloride_Powder_100g_0", "name": "Sodium Chloride Powder 100g", "x": 20, "y": 7, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Powders/BP_Sodium_Chloride_100g.BP_Sodium_Chloride_100g_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle02_100ml.png", "thumbnailImage": "containers/sodium_chloride_powder_100g.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "EDTA_Powder_100g_0", "name": "EDTA Powder 100g", "x": 18, "y": 5, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Powders/BP_EDTA_100g.BP_EDTA_100g_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle02_100ml.png", "thumbnailImage": "containers/edta_powder_100g.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Potassium_Chloride_Powder_100g_0", "name": "Potassium Chloride Powder 100g", "x": 16, "y": 5, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Powders/BP_Potassium_Chloride_100g.BP_Potassium_Chloride_100g_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle02_100ml.png", "thumbnailImage": "containers/potassium_chloride_powder_100g.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Centrifuge_Tube_50ml_0", "name": "Centrifuge Tube 50ml", "x": 16, "y": 7, "w": 4, "h": 3, "ueBlueprint": "/Game/Instruments/Centrifuge/BP_Dispenser_Centrifuge_Tube_50ml.BP_Dispenser_Centrifuge_Tube_50ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/dispenser_centrifuge_tube_50ml.png", "thumbnailImage": "containers/centrifuge_tube_50ml.png", "isSurface": false, "requiresSurface": true, "items": [] } ] }, { "id": "Table_60_x_45_0", "name": "Table 60 x 45", "x": 28, "y": 4, "w": 12, "h": 9, "ueBlueprint": "/Game/Instruments/Tables/BP_Table_Stand_Small.BP_Table_Stand_Small_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "furniture/table_stand.png", "thumbnailImage": "furniture/table_stand.png", "isSurface": true, "requiresSurface": true, "items": [ { "id": "Dispenser_Paper_Table_0", "name": "Dispenser Paper Table", "x": 1, "y": 1, "w": 4, "h": 4, "ueBlueprint": "/Game/Instruments/Sanitising/BP_Dispenser_Paper_Towel.BP_Dispenser_Paper_Towel_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "cleaning/dispenser_paper_table.png", "thumbnailImage": "cleaning/dispenser_paper_table.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Wipes_IPA_0", "name": "Wipes IPA", "x": 6, "y": 1, "w": 5, "h": 3, "ueBlueprint": "/Game/Instruments/Sanitising/BP_Dispenser_Wipes.BP_Dispenser_Wipes_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "cleaning/wipes_ipa.png", "thumbnailImage": "cleaning/wipes_ipa.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Ethanol_Spray_Bottle_0", "name": "Ethanol Spray Bottle", "x": 2, "y": 6, "w": 3, "h": 2, "ueBlueprint": "/Game/Instruments/Sanitising/BP_Spray.BP_Spray_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "cleaning/spray_bottle.png", "thumbnailImage": "cleaning/spray_bottle.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Water_Wash_Bottle_0", "name": "Water Wash Bottle", "x": 6, "y": 6, "w": 3, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/SqueezeBottles/BP_Squeeze_Bottle_Water.BP_Squeeze_Bottle_Water_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/wash_bottle_water_5l.png", "thumbnailImage": "containers/wash_bottle_water_5l.png", "isSurface": false, "requiresSurface": true, "items": [] } ] }, { "id": "Bio_Bin_6L_0", "name": "Bio Bin 6L", "x": 35, "y": 14, "w": 5, "h": 4, "ueBlueprint": "/Game/Instruments/Bins/BP_Bin_6L_Bio.BP_Bin_6L_Bio_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "cleaning/bio_bin_6l.png", "thumbnailImage": "cleaning/bio_bin_6l.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Table_60_x_45_1", "name": "Table 60 x 45", "x": 31, "y": 24, "w": 9, "h": 12, "ueBlueprint": "/Game/Instruments/Tables/BP_Table_Stand_Small.BP_Table_Stand_Small_C", "absoluteRotation": 90, "relativeRotation": 90, "gridImage": "furniture/table_stand.png", "thumbnailImage": "furniture/table_stand.png", "isSurface": true, "requiresSurface": true, "items": [ { "id": "Analytical_Scale_-_Calibration_Weights_0", "name": "Analytical Scale - Calibration Weights", "x": 6, "y": 1, "w": 2, "h": 6, "ueBlueprint": "/Game/Instruments/Measuring/WeighingScale/CalibrationWeights/BP_Calibration_Weights_Box.BP_Calibration_Weights_Box_C", "absoluteRotation": 90, "relativeRotation": 0, "gridImage": "devices/analytical_scale_weights.png", "thumbnailImage": "devices/analytical_scale_weights.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Seropipette_10ml_0", "name": "Seropipette 10ml", "x": 4, "y": 0, "w": 1, "h": 8, "ueBlueprint": "/Game/Instruments/Pipettes/Dispensers/BP_Dispenser_Seropips_10ml.BP_Dispenser_Seropips_10ml_C", "absoluteRotation": 90, "relativeRotation": 0, "gridImage": "containers/dispenser_seropipette_10ml.png", "thumbnailImage": "containers/seropip_10ml.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Spatula_Case_0", "name": "Spatula Case", "x": 3, "y": 9, "w": 6, "h": 3, "ueBlueprint": "/Game/Instruments/Spatula/BP_Spatula_Set.BP_Spatula_Set_C", "absoluteRotation": 90, "relativeRotation": 0, "gridImage": "containers/spatula_case.png", "thumbnailImage": "containers/spatula_case.png", "isSurface": false, "requiresSurface": true, "parts": [ { "slot": "Snap1", "ueBlueprint": "/Game/Instruments/Spatula/BP_Spatula_1g.BP_Spatula_1g_C", "parts": [] }, { "slot": "Snap10", "ueBlueprint": "/Game/Instruments/Spatula/BP_Spatula_10g.BP_Spatula_10g_C", "parts": [] }, { "slot": "Snap50", "ueBlueprint": "/Game/Instruments/Spatula/BP_Spatula_50g.BP_Spatula_50g_C", "parts": [] } ], "items": [] }, { "id": "Weigh_Boat_100g_0", "name": "Weigh Boat 100g", "x": 0, "y": 9, "w": 3, "h": 3, "ueBlueprint": "/Game/Instruments/Containers/WeighingBoats/BP_Dispenser_Weighing_Boat_100ml.BP_Dispenser_Weighing_Boat_100ml_C", "absoluteRotation": 90, "relativeRotation": 0, "gridImage": "containers/weigh_boat_100ml.png", "thumbnailImage": "containers/weigh_boat_100ml.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Funnel_254ml_0", "name": "Funnel 254ml", "x": 0, "y": 0, "w": 3, "h": 4, "ueBlueprint": "/Game/Instruments/Funnel/BP_Dispenser_Funnel_254ml.BP_Dispenser_Funnel_254ml_C", "absoluteRotation": 90, "relativeRotation": 0, "gridImage": "containers/funnel_254ml.png", "thumbnailImage": "containers/funnel_254ml.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Weigh_Boat_7g_0", "name": "Weigh Boat 7g", "x": 0, "y": 7, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/WeighingBoats/BP_Dispenser_Weighing_Boat_7ml.BP_Dispenser_Weighing_Boat_7ml_C", "absoluteRotation": 90, "relativeRotation": 0, "gridImage": "containers/weigh_boat_7ml.png", "thumbnailImage": "containers/weigh_boat_7ml.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Funnel_64ml_0", "name": "Funnel 64ml", "x": 1, "y": 4, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Funnel/BP_Dispenser_Funnel_64ml.BP_Dispenser_Funnel_64ml_C", "absoluteRotation": 90, "relativeRotation": 0, "gridImage": "containers/funnel_64ml.png", "thumbnailImage": "containers/funnel_64ml.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Pipette_Controller_0", "name": "Pipette Controller", "x": 4, "y": 8, "w": 4, "h": 1, "ueBlueprint": "/Game/Instruments/Pipettes/BP_Pipette_Controller.BP_Pipette_Controller_C", "absoluteRotation": 90, "relativeRotation": 0, "gridImage": "devices/pipette_controller.png", "thumbnailImage": "devices/pipette_controller.png", "isSurface": false, "requiresSurface": false, "items": [] } ] } ], "thumbnailImage": "", "gridImage": "", "parts": [] } }, { "version": "v2", "name": "Creating a Dilution Series", "steps": [ "Learn some basic chemistry, creating a dilution series using a pipette.", "Pinch at the box labelled 'microcentrifuge tube' and fill a rack with them. ", "Pinch at the box labelled 'antibodies' to remove a tube and place it in the other rack.", "Pick up the blue micropipette from the stand of pipettes by grabbing at it. ", "Insert the blue pipette into the pipette tip box to pick up a tip. ", "Adjust the 1000uL micropipette volume to 900uL using the + and - toggles.", "Dip the tip of the pipette inside the flow buffer solution and withdraw it into the pipette.", "Once the numbers on the pop up above the pipette reaches 900uL, you can move the pipette from the bottle and push the liquid out into the top left micropipette in the rack.", "Return the blue 1000uL pipette and retrieve the yellow 200uL pipette from the rack", "Adjust the 200uL micropipette volume to 100uL using the + and - toggles.", "Dip the tip of the pipette inside the antibody vial and withdraw it into the pipette.", "Dispense 100uL antibodies into the top left micropipette in the rack with the flow buffer to create a 1:10 dilution. You should label this tube 1:10 in the real lab. ", "Remove the tip and close the tube lid.", "Vortex the tube for 5-10 seconds to mix thoroughly.", "Retrieve a new tip for the 200uL micropipette", "Remove 100uL of the 1:10 solution from the first microcentrifuge tube and transfer to the next tube along (tube 2), which in the lab would be labelled 1:100. ", "Retrieving a new pipette tip for the 1000uL pipette, adjust to 900uL and transfer 900uL flow buffer into the 1:100 tube (tube 2).", "Vortex the tube for 5-10 seconds to mix thoroughly.", "Repeat this process for as many dilutions as needed (e.g. 1:1000, 1:10000), each time transferring 100 uL from the previous dilution to a new micro tube with 900 uL of flow buffer and vortexing to mix.", "In the laboratory flow buffer-antibody staining solutions of different dilutions would be added to cells before performing the phenotyping. Running the antibody at different dilutions (a “titration”) helps determine the best working concentration for that specific antibody, cell type and experiment." ], "layout": { "id": "main", "name": "Main grid", "x": 0, "y": 0, "w": 40, "h": 40, "ueBlueprint": "", "relativeRotation": 0, "absoluteRotation": 0, "isSurface": true, "requiresSurface": false, "items": [ { "id": "Table_180_x_60_0", "name": "Table 180 x 60", "x": 2, "y": 5, "w": 36, "h": 12, "ueBlueprint": "/Game/Instruments/Tables/BP_Table_180x60.BP_Table_180x60_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "furniture/table_180x60.png", "thumbnailImage": "furniture/table_large.png", "isSurface": true, "requiresSurface": true, "items": [ { "id": "Bio_Bin_1L_0", "name": "Bio Bin 1L", "x": 30, "y": 1, "w": 5, "h": 4, "ueBlueprint": "/Game/Instruments/Bins/BP_Bin_1L_Bio.BP_Bin_1L_Bio_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "cleaning/bio_bin_1l.png", "thumbnailImage": "cleaning/bio_bin_1l.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Bottle_500ml_0", "name": "Bottle 500ml", "x": 27, "y": 1, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/GL45/BP_Bottle_GL45_500ml.BP_Bottle_GL45_500ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle03_500ml.png", "thumbnailImage": "containers/bottle03_500ml.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Centrifuge_Tube_15ml_0", "name": "Centrifuge Tube 15ml", "x": 22, "y": 1, "w": 4, "h": 3, "ueBlueprint": "/Game/Instruments/Centrifuge/BP_Dispenser_Centrifuge_Tube_15ml.BP_Dispenser_Centrifuge_Tube_15ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/dispenser_centrifuge_tube_15ml.png", "thumbnailImage": "containers/centrifuge_tube_15ml.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Pipette_tip_box_200ul_0", "name": "Pipette tip box 200ul", "x": 1, "y": 1, "w": 3, "h": 3, "ueBlueprint": "/Game/Instruments/Pipettes/Dispensers/BP_Dispenser_Micro_Pipettes_200.BP_Dispenser_Micro_Pipettes_200_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/dispenser_pipette_tips_200ul.png", "thumbnailImage": "containers/dispenser_pipette_tips_200ul.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Pipette_tip_box_1000ul_0", "name": "Pipette tip box 1000ul", "x": 1, "y": 4, "w": 3, "h": 3, "ueBlueprint": "/Game/Instruments/Pipettes/Dispensers/BP_Dispenser_Micro_Pipettes_1000.BP_Dispenser_Micro_Pipettes_1000_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/dispenser_pipette_tips_1000ul.png", "thumbnailImage": "containers/dispenser_pipette_tips_1000ul.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Pipettes_Mechanical_10ul,_25ul,_100ul,_1000ul_0", "name": "Pipettes Mechanical 10ul, 25ul, 100ul, 1000ul", "x": 5, "y": 1, "w": 5, "h": 3, "ueBlueprint": "/Game/Instruments/Pipettes/BP_Mechanical_Pipette_Stand.BP_Mechanical_Pipette_Stand_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/mechanical_pipette_rack.png", "thumbnailImage": "devices/mechanical_pipette_rack.png", "isSurface": false, "requiresSurface": true, "parts": [ { "slot": "Pipette10Snap", "ueBlueprint": "/Game/Instruments/Pipettes/BP_Mechanical_Pipette_Controller_10.BP_Mechanical_Pipette_Controller_10_C" }, { "slot": "Pipette20Snap", "ueBlueprint": "/Game/Instruments/Pipettes/BP_Mechanical_Pipette_Controller_20.BP_Mechanical_Pipette_Controller_20_C" }, { "slot": "Pipette200Snap", "ueBlueprint": "/Game/Instruments/Pipettes/BP_Mechanical_Pipette_Controller_200.BP_Mechanical_Pipette_Controller_200_C" }, { "slot": "Pipette1000Snap", "ueBlueprint": "/Game/Instruments/Pipettes/BP_Mechanical_Pipette_Controller_1000.BP_Mechanical_Pipette_Controller_1000_C" } ], "items": [] }, { "id": "Seropipette_10ml_0", "name": "Seropipette 10ml", "x": 27, "y": 10, "w": 8, "h": 1, "ueBlueprint": "/Game/Instruments/Pipettes/Dispensers/BP_Dispenser_Seropips_10ml.BP_Dispenser_Seropips_10ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/dispenser_seropipette_10ml.png", "thumbnailImage": "containers/seropip_10ml.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Ethanol_Spray_Bottle_0", "name": "Ethanol Spray Bottle", "x": 31, "y": 6, "w": 3, "h": 2, "ueBlueprint": "/Game/Instruments/Sanitising/BP_Spray.BP_Spray_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "cleaning/spray_bottle.png", "thumbnailImage": "cleaning/spray_bottle.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Pipette_Controller_0", "name": "Pipette Controller", "x": 28, "y": 4, "w": 1, "h": 4, "ueBlueprint": "/Game/Instruments/Pipettes/BP_Pipette_Controller.BP_Pipette_Controller_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/pipette_controller.png", "thumbnailImage": "devices/pipette_controller.png", "isSurface": false, "requiresSurface": false, "items": [] }, { "id": "Flow_Buffer_100ml_0", "name": "Flow Buffer 100ml", "x": 5, "y": 5, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Flow_Buffer_100ml.BP_Bottle_Flow_Buffer_100ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle02_100ml.png", "thumbnailImage": "containers/bottle100ml_flow_buffer.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Microcentrifuge_Tube_1.5ml_0", "name": "Microcentrifuge Tube 1.5ml", "x": 1, "y": 8, "w": 3, "h": 2, "ueBlueprint": "/Game/Instruments/Centrifuge/BP_Dispenser_Microcentrifuge_Tube_1halfml.BP_Dispenser_Microcentrifuge_Tube_1halfml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/dispenser_microcentrifuge_tube_one_half_ml.png", "thumbnailImage": "containers/microcentrifuge_tube_one_half_ml.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Tube_Rack_Micro_0", "name": "Tube Rack Micro", "x": 13, "y": 8, "w": 3, "h": 1, "ueBlueprint": "/Game/Instruments/Centrifuge/TubeRacks/BP_Centrifuge_Tube_Rack_Micro.BP_Centrifuge_Tube_Rack_Micro_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "other/tube_rack_micro.png", "thumbnailImage": "other/tube_rack_micro.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Tube_Rack_Micro_1", "name": "Tube Rack Micro", "x": 13, "y": 9, "w": 3, "h": 1, "ueBlueprint": "/Game/Instruments/Centrifuge/TubeRacks/BP_Centrifuge_Tube_Rack_Micro.BP_Centrifuge_Tube_Rack_Micro_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "other/tube_rack_micro.png", "thumbnailImage": "other/tube_rack_micro.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Tube_Rack_15ml_1", "name": "Tube Rack 15ml", "x": 22, "y": 9, "w": 3, "h": 1, "ueBlueprint": "/Game/Instruments/Centrifuge/TubeRacks/BP_Centrifuge_Tube_Rack_15ml.BP_Centrifuge_Tube_Rack_15ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "other/tube_rack_15ml.png", "thumbnailImage": "other/tube_rack_15ml.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Tube_Rack_15ml_0", "name": "Tube Rack 15ml", "x": 22, "y": 8, "w": 3, "h": 1, "ueBlueprint": "/Game/Instruments/Centrifuge/TubeRacks/BP_Centrifuge_Tube_Rack_15ml.BP_Centrifuge_Tube_Rack_15ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "other/tube_rack_15ml.png", "thumbnailImage": "other/tube_rack_15ml.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Antibodies_0", "name": "Antibodies", "x": 8, "y": 5, "w": 3, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Dispensers/BP_Dispenser_Vial_Antibodies.BP_Dispenser_Vial_Antibodies_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/dispenser_antibodies.png", "thumbnailImage": "containers/live_staining_solution.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Cell_Suspension_50ml_0", "name": "Cell Suspension 50ml", "x": 17, "y": 1, "w": 4, "h": 3, "ueBlueprint": "/Game/Instruments/Containers/Dispensers/BP_Dispenser_Capped_Tube_Cell_Suspension_50ml.BP_Dispenser_Capped_Tube_Cell_Suspension_50ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/dispenser_cell_suspension_50ml.png", "thumbnailImage": "containers/cell_suspension_50ml.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Vortex_0", "name": "Vortex", "x": 12, "y": 1, "w": 5, "h": 5, "ueBlueprint": "/Game/Instruments/Vortex/BP_Vortex.BP_Vortex_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/vortex.png", "thumbnailImage": "devices/vortex.png", "isSurface": false, "requiresSurface": true, "items": [] } ] } ], "thumbnailImage": "", "gridImage": "", "parts": [] } } ] } ] }