{ "version": "v3", "compatibility": "0.1.3", "collections": [ { "name": "Calibration of basic laboratory equipment", "sops": [ { "version": "v2", "name": "Calibrating a mechanical pipette", "steps": [ "Verify the accuracy of the analytical balance using calibration weights.", "Ensure the balance is in a draft-free environment to prevent air currents from affecting the measurements.", "Place a weighing boat/vessel/tube on the balance and tare it to zero.", "Select the appropriate pipette tips for the pipette being calibrated.", "Attach a new, clean pipette tip to the pipette.", "Measure the temperature of the distilled water and ensure it is within the range of 20-25°C.", "Set the pipette to the desired volume (e.g., 10 µL, 100 µL, 1000 µL).", "Pre-wet the pipette tip by aspirating and dispensing distilled water three times.", "Aspirate the desired volume of distilled water.", "Dispense the water into the pre-tared weighing boat or vessel on the analytical balance.", "Record the weight of the dispensed water.", "Repeat the process for a minimum of 10 measurements for each volume setting.", "Calculate the mean volume dispensed, standard deviation, and coefficient of variation for each volume setting.", "Compare the results to the manufacturer’s specifications for accuracy and precision.", "Record all measurements, including the volume setting, weight of dispensed water, and calculated volumes, in the calibration logbook.", "Ensure all data is clearly labelled and organized.", "Convert the weight of the dispensed water to volume using the density of water at the measured temperature (approximately 0.9982 g/mL at 20°C).", "Verify that the pipette meets the manufacturer’s specifications for accuracy and precision.", "Verify that the pipette meets the manufacturer’s specifications for accuracy and precision." ], "layout": { "id": "main", "name": "Main grid", "x": 0, "y": 0, "w": 40, "h": 40, "ueBlueprint": "", "relativeRotation": 0, "absoluteRotation": 0, "isSurface": true, "requiresSurface": false, "items": [ { "id": "Table_120_x_45_1", "name": "Table 120 x 45", "x": 3, "y": 2, "w": 24, "h": 9, "ueBlueprint": "/Game/Instruments/Tables/BP_Table_Stand_Small_Thin.BP_Table_Stand_Small_Thin_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "furniture/table_thin.png", "thumbnailImage": "furniture/table_thin.png", "isSurface": true, "requiresSurface": true, "items": [ { "id": "Water_Bath_0", "name": "Water Bath", "x": 1, "y": 1, "w": 11, "h": 7, "ueBlueprint": "/Game/Instruments/WaterBath/BP_Water_Bath.BP_Water_Bath_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/water_bath.png", "thumbnailImage": "devices/water_bath.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Pipettes_Mechanical_10ul,_25ul,_100ul,_1000ul_0", "name": "Pipettes Mechanical 10ul, 25ul, 100ul, 1000ul", "x": 17, "y": 1, "w": 5, "h": 3, "ueBlueprint": "/Game/Instruments/Pipettes/BP_Mechanical_Pipette_Stand.BP_Mechanical_Pipette_Stand_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/mechanical_pipette_rack.png", "thumbnailImage": "devices/mechanical_pipette_rack.png", "isSurface": false, "requiresSurface": true, "parts": [ { "slot": "Pipette10Snap", "ueBlueprint": "/Game/Instruments/Pipettes/BP_Mechanical_Pipette_Controller_10.BP_Mechanical_Pipette_Controller_10_C" }, { "slot": "Pipette20Snap", "ueBlueprint": "/Game/Instruments/Pipettes/BP_Mechanical_Pipette_Controller_20.BP_Mechanical_Pipette_Controller_20_C" }, { "slot": "Pipette200Snap", "ueBlueprint": "/Game/Instruments/Pipettes/BP_Mechanical_Pipette_Controller_200.BP_Mechanical_Pipette_Controller_200_C" }, { "slot": "Pipette1000Snap", "ueBlueprint": "/Game/Instruments/Pipettes/BP_Mechanical_Pipette_Controller_1000.BP_Mechanical_Pipette_Controller_1000_C" } ], "items": [] }, { "id": "Distilled_Water_1L_0", "name": "Distilled Water 1L", "x": 13, "y": 1, "w": 3, "h": 3, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Distilled_Water_1L.BP_Bottle_Distilled_Water_1L_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle01_1l.png", "thumbnailImage": "containers/bottle1l_distilled_water.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Pipette_tip_box_20ul_0", "name": "Pipette tip box 20ul", "x": 13, "y": 5, "w": 3, "h": 3, "ueBlueprint": "/Game/Instruments/Pipettes/Dispensers/BP_Dispenser_Micro_Pipettes_20.BP_Dispenser_Micro_Pipettes_20_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/dispenser_pipette_tips_20ul.png", "thumbnailImage": "containers/dispenser_pipette_tips_20ul.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Microcentrifuge_Tube_1.5ml_0", "name": "Microcentrifuge Tube 1.5ml", "x": 21, "y": 6, "w": 3, "h": 2, "ueBlueprint": "/Game/Instruments/Centrifuge/BP_Dispenser_Microcentrifuge_Tube_1halfml.BP_Dispenser_Microcentrifuge_Tube_1halfml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/dispenser_microcentrifuge_tube_one_half_ml.png", "thumbnailImage": "containers/microcentrifuge_tube_one_half_ml.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Pipette_tip_box_1000ul_0", "name": "Pipette tip box 1000ul", "x": 17, "y": 5, "w": 3, "h": 3, "ueBlueprint": "/Game/Instruments/Pipettes/Dispensers/BP_Dispenser_Micro_Pipettes_1000.BP_Dispenser_Micro_Pipettes_1000_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/dispenser_pipette_tips_1000ul.png", "thumbnailImage": "containers/dispenser_pipette_tips_1000ul.png", "isSurface": false, "requiresSurface": true, "items": [] } ] }, { "id": "Table_120_x_45_0", "name": "Table 120 x 45", "x": 27, "y": 2, "w": 9, "h": 24, "ueBlueprint": "/Game/Instruments/Tables/BP_Table_Stand_Small_Thin.BP_Table_Stand_Small_Thin_C", "absoluteRotation": 90, "relativeRotation": 90, "gridImage": "furniture/table_thin.png", "thumbnailImage": "furniture/table_thin.png", "isSurface": true, "requiresSurface": true, "items": [ { "id": "Analytical_Scale_0", "name": "Analytical Scale", "x": 1, "y": 14, "w": 7, "h": 7, "ueBlueprint": "/Game/Instruments/Measuring/WeighingScale/BP_Analytical_Scale.BP_Analytical_Scale_C", "absoluteRotation": 90, "relativeRotation": 0, "gridImage": "devices/analytical_scale.png", "thumbnailImage": "devices/analytical_scale.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Analytical_Scale_-_Calibration_Weights_0", "name": "Analytical Scale - Calibration Weights", "x": 5, "y": 7, "w": 2, "h": 6, "ueBlueprint": "/Game/Instruments/Measuring/WeighingScale/CalibrationWeights/BP_Calibration_Weights_Box.BP_Calibration_Weights_Box_C", "absoluteRotation": 90, "relativeRotation": 0, "gridImage": "devices/analytical_scale_weights.png", "thumbnailImage": "devices/analytical_scale_weights.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Dispenser_Paper_Table_0", "name": "Dispenser Paper Table", "x": 4, "y": 1, "w": 4, "h": 4, "ueBlueprint": "/Game/Instruments/Sanitising/BP_Dispenser_Paper_Towel.BP_Dispenser_Paper_Towel_C", "absoluteRotation": 0, "relativeRotation": 270, "gridImage": "cleaning/dispenser_paper_table.png", "thumbnailImage": "cleaning/dispenser_paper_table.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Ethanol_Spray_Bottle_0", "name": "Ethanol Spray Bottle", "x": 1, "y": 2, "w": 2, "h": 3, "ueBlueprint": "/Game/Instruments/Sanitising/BP_Spray.BP_Spray_C", "absoluteRotation": 270, "relativeRotation": 180, "gridImage": "cleaning/spray_bottle.png", "thumbnailImage": "cleaning/spray_bottle.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Digital_Thermometer_0", "name": "Digital Thermometer", "x": 2, "y": 6, "w": 5, "h": 1, "ueBlueprint": "/Game/Instruments/Thermometer/BP_Handheld_Thermometer.BP_Handheld_Thermometer_C", "absoluteRotation": 0, "relativeRotation": 270, "gridImage": "devices/digital_thermometer.png", "thumbnailImage": "devices/digital_thermometer.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Tube_Rack_Micro_0", "name": "Tube Rack Micro", "x": 2, "y": 8, "w": 1, "h": 3, "ueBlueprint": "/Game/Instruments/Centrifuge/TubeRacks/BP_Centrifuge_Tube_Rack_Micro.BP_Centrifuge_Tube_Rack_Micro_C", "absoluteRotation": 90, "relativeRotation": 0, "gridImage": "other/tube_rack_micro.png", "thumbnailImage": "other/tube_rack_micro.png", "isSurface": false, "requiresSurface": true, "items": [] } ] }, { "id": "Bio_Bin_6L_0", "name": "Bio Bin 6L", "x": 27, "y": 27, "w": 4, "h": 5, "ueBlueprint": "/Game/Instruments/Bins/BP_Bin_6L_Bio.BP_Bin_6L_Bio_C", "absoluteRotation": 90, "relativeRotation": 90, "gridImage": "cleaning/bio_bin_6l.png", "thumbnailImage": "cleaning/bio_bin_6l.png", "isSurface": false, "requiresSurface": true, "items": [] } ], "thumbnailImage": "", "gridImage": "", "parts": [] } }, { "name": "PH meter calibration", "steps": [ "Purpose and scope: pH meter calibration is important as it ensures accurate pH measurements by adjusting the meter against standardized buffer solutions.", "Turn on Microbiological/Biological Safety Cabinet (MSC/BSC). This procedure should ideally be performed in a fume hood if possible.", "Turn on MSC light.", "Turn on laminar airflow (fan).", "Use buttons to lift MSC glass sash.", "Spray hands with ethanol before working inside the MSC.", "Place beaker underneath pH meter and turn on the pH meter.", "Remove protective moistened tip end cap and place on surface.", "Rinse electrode of the pH meter in sterile distilled water into the beaker.", "Press CAL (Calibrate button).", "Move water filled beaker to one side.", "Remove cap from pH 7 solution bottle and place below pH meter.", "Dip pH probe into sterile uncapped pH 7 solution and note down the pH reading", " If it's reading is more than 0.05 pH away from the standard buffer solution, the pH meter will require re-calibration.", "Remove pH 7 solution from below probe.", "Immediately place beaker of distilled water below probe and rinse probe well with distilled water.", "Remove beaker from below probe and replace with uncapped pH 4 solution.", "Press CAL (Calibrate) and dip pH probe into sterile uncapped pH 4 solution and note down the pH reading.", "Remove pH 4 solution from below probe.", "Place beaker of distilled water below probe and rinse probe well with distilled water.", "Remove beaker from below probe and replace with uncapped pH 10 solution.", "Press CAL (Calibrate) and dip pH probe into sterile uncapped pH 10 solution and note down the pH reading.", "Remove pH 10 solution from below probe.", "Place beaker of distilled water below probe and rinse probe well with distilled water.", "Recap probe with protective moistened dip end cap. ", " Once the pH probe and meter are calibrated you can start measuring unknown solutions using the method below.", "Remove lid from solution of unknown pH.", "Grab the serological pipette dispenser with one hand and grab serological pipette controller with the other hand.", "Attach serological pipette to controller by hovering it over the snap slot until it clicks into place, then release.", "With your middle finger extended and your index finger pressed down on the withdraw button, withdraw liquid into the serological pipette controller whilst holding the pipette in the liquid. Remove enough to fill the bottom of the beaker. ", "Then with your index finger extended and middle finger down on the expel button, expel enough liquid to fill the bottom of the beaker.", "Remove used serological pipette from controller once all liquid has been expelled and dispose of it in the contaminated waste bin.", "Re-cap the solution of unknown pH and place the pipette controller on the surface.", "Place beaker of solution to be measured below pH probe on stand.", "Re-press CAL on pH meter to perform a measurement.", "Remove protective moistened dip end cap and place on surface.", "Dip pH probe into unknown solution in beaker and note down the pH reading. Press Enter.", "Remove pH probe from the solution.", "Move unknown solution to the side.", "Rinse electrode and pH meter in sterile distilled water into the distilled water beaker.", "Re-measure solution of unknown pH three times in total for accuracy, rinsing with distilled water into water beaker between each measurement.", "Move unknown solution to the side and dispose of/store as intended.", "Rinse electrode and pH meter in sterile distilled water into the distilled water beaker a final time.", "Recap probe with protective moistened dip end cap. ", "Switch off pH meter." ], "layout": { "id": "main", "name": "Main grid", "x": 0, "y": 0, "w": 40, "h": 40, "ueBlueprint": "", "relativeRotation": 0, "absoluteRotation": 0, "isSurface": true, "requiresSurface": false, "items": [ { "id": "MSC_Small_0", "name": "MSC Small", "x": 1, "y": 1, "w": 28, "h": 13, "ueBlueprint": "/Game/Instruments/MSC/BP_MSC_Herasafe_Standing.BP_MSC_Herasafe_Standing_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/msc.png", "thumbnailImage": "devices/msc.png", "isSurface": true, "requiresSurface": false, "items": [ { "id": "Beaker_250ml_0", "name": "Beaker 250ml", "x": 2, "y": 4, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Beakers/BP_Beaker_250ml.BP_Beaker_250ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/beaker_250ml.png", "thumbnailImage": "containers/beaker_250ml.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Beaker_250ml_1", "name": "Beaker 250ml", "x": 2, "y": 6, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Beakers/BP_Beaker_250ml.BP_Beaker_250ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/beaker_250ml.png", "thumbnailImage": "containers/beaker_250ml.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Beaker_250ml_2", "name": "Beaker 250ml", "x": 2, "y": 8, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Beakers/BP_Beaker_250ml.BP_Beaker_250ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/beaker_250ml.png", "thumbnailImage": "containers/beaker_250ml.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Acetate_Buffer_100ml_0", "name": "Acetate Buffer 100ml", "x": 21, "y": 1, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Acetate_Buffer_100ml.BP_Bottle_Acetate_Buffer_100ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle02_100ml.png", "thumbnailImage": "containers/bottle100ml_acetate_buffer.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Tris_Buffer_100ml_0", "name": "Tris Buffer 100ml", "x": 19, "y": 1, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Tris_Buffer_100ml.BP_Bottle_Tris_Buffer_100ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle02_100ml.png", "thumbnailImage": "containers/bottle100ml_tris_buffer.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Potassium_Nitrate_100ml_0", "name": "Potassium Nitrate 100ml", "x": 17, "y": 1, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Potassium_Nitrate_100ml.BP_Bottle_Potassium_Nitrate_100ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle02_100ml.png", "thumbnailImage": "containers/bottle100ml_potassium_nitrate.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Ph_Meter_0", "name": "Ph Meter", "x": 6, "y": 2, "w": 7, "h": 5, "ueBlueprint": "/Game/Instruments/Measuring/pHReader/BP_pH_Reader.BP_pH_Reader_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/ph_meter.png", "thumbnailImage": "devices/ph_meter.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Water_Wash_Bottle_0", "name": "Water Wash Bottle", "x": 2, "y": 2, "w": 3, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/SqueezeBottles/BP_Squeeze_Bottle_Water.BP_Squeeze_Bottle_Water_C", "absoluteRotation": 180, "relativeRotation": 180, "gridImage": "containers/wash_bottle_water_5l.png", "thumbnailImage": "containers/wash_bottle_water_5l.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Distilled_Water_1L_0", "name": "Distilled Water 1L", "x": 23, "y": 2, "w": 3, "h": 3, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Distilled_Water_1L.BP_Bottle_Distilled_Water_1L_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle01_1l.png", "thumbnailImage": "containers/bottle1l_distilled_water.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Seropipette_50ml_0", "name": "Seropipette 50ml", "x": 15, "y": 3, "w": 8, "h": 2, "ueBlueprint": "/Game/Instruments/Pipettes/Dispensers/BP_Dispenser_Seropips_50ml.BP_Dispenser_Seropips_50ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/dispenser_seropipette_50ml.png", "thumbnailImage": "containers/seropip_50ml.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Pipette_Controller_0", "name": "Pipette Controller", "x": 22, "y": 6, "w": 4, "h": 1, "ueBlueprint": "/Game/Instruments/Pipettes/BP_Pipette_Controller.BP_Pipette_Controller_C", "absoluteRotation": 90, "relativeRotation": 90, "gridImage": "devices/pipette_controller.png", "thumbnailImage": "devices/pipette_controller.png", "isSurface": false, "requiresSurface": false, "items": [] }, { "id": "pH4_solution_100ml_0", "name": "pH4 solution 100ml", "x": 20, "y": 8, "w": 1, "h": 1, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_pH_4_Solution.BP_Bottle_pH_4_Solution_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle03_100ml.png", "thumbnailImage": "containers/bottle100ml_ph4.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "pH10_solution_100ml_0", "name": "pH10 solution 100ml", "x": 22, "y": 8, "w": 1, "h": 1, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_pH_10_Solution.BP_Bottle_pH_10_Solution_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle03_100ml.png", "thumbnailImage": "containers/bottle100ml_ph10.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "pH7_solution_100ml_0", "name": "pH7 solution 100ml", "x": 24, "y": 8, "w": 1, "h": 1, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_pH_7_Solution.BP_Bottle_pH_7_Solution_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle03_100ml.png", "thumbnailImage": "containers/bottle100ml_ph7.png", "isSurface": false, "requiresSurface": true, "items": [] } ] }, { "id": "Table_60_x_45_0", "name": "Table 60 x 45", "x": 29, "y": 15, "w": 9, "h": 12, "ueBlueprint": "/Game/Instruments/Tables/BP_Table_Stand_Small.BP_Table_Stand_Small_C", "absoluteRotation": 90, "relativeRotation": 90, "gridImage": "furniture/table_stand.png", "thumbnailImage": "furniture/table_stand.png", "isSurface": true, "requiresSurface": true, "items": [ { "id": "Ethanol_Spray_Bottle_0", "name": "Ethanol Spray Bottle", "x": 2, "y": 1, "w": 3, "h": 2, "ueBlueprint": "/Game/Instruments/Sanitising/BP_Spray.BP_Spray_C", "absoluteRotation": 0, "relativeRotation": 270, "gridImage": "cleaning/spray_bottle.png", "thumbnailImage": "cleaning/spray_bottle.png", "isSurface": false, "requiresSurface": true, "items": [] } ] }, { "id": "Bio_Bin_6L_0", "name": "Bio Bin 6L", "x": 24, "y": 15, "w": 5, "h": 4, "ueBlueprint": "/Game/Instruments/Bins/BP_Bin_6L_Bio.BP_Bin_6L_Bio_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "cleaning/bio_bin_6l.png", "thumbnailImage": "cleaning/bio_bin_6l.png", "isSurface": false, "requiresSurface": true, "items": [] } ], "thumbnailImage": "", "gridImage": "", "parts": [] } }, { "name": "Calibrating an Analytical Scale", "steps": [ "Purpose and Scope: Initial training into the calibration of a weighing scale prior to weighing liquids for accuracy post pipetting. ", "Check for visible damage, loose components, or debris on the weighing platform.", "Ensure the draft shield door on the scale is closed. ", "Turn on the analytical scale and allow it to stabilise.", "Check the scale is balanced by inspecting the indicator. The bubble should be in the centre of the indicator ring. If not this can be adjusted by tapping each wheel. ", "Check that the measurement mode is in grams.", "Press the Function, then the Tare button to access the calibration screen.", "Gently open the draft shield door.", "Pick up the smallest/lightest calibration weight from the box and place it in the centre of the scale. ", "Gently close the draft shield door by touching the handle.", "The scale should show the weight but wait a few seconds until the balance stabilises.", "Make a note of the weight in a calibration book.", "Gently open the draft shield door by touching the handle.", "Remove the weight from the scale and close the draft shield door.", "Re-TARE the scale.", "Repeat the calibration/calibration weight weighing process with the other weights in order lightest-heaviest.", "Once all the weights have been calibrated , double tap the function button to take you back to the sample measuring mode.", "Press the TARE button. ", "Gently open the draft shield door. ", "Place the empty 50ml centrifuge tube on the scale and gently close the draft shield door. ", "Make a note of the weight before adding liquid.", "Press TARE. ", "Gently open the draft shield door and remove the empty centrifuge tube. ", "Gently close the draft shield door. ", "Using a serological pipette and pipette controller, transfer 36ml of FBS into the centrifuge tube. ", "Gently open the draft shield door. ", "Place the filled 50ml centrifuge tube on the scale and gently close the draft shield door. ", "Make a note of the weight post FBS. ", "Gently open the draft shield door and remove the filled centrifuge tube. ", "Gently close the draft shield door.", "Using a 1000 µL mechanical pipette and 1000 µL tips, transfer 4 mL of DMSO, adding 1 mL at a time and pipetting up and down to mix, into the centrifuge tube to bring the total volume to 40 mL.", "Gently open the draft shield door.", "Place the filled 50ml centrifuge tube on the scale and gently close the draft shield door.", "Make a note of the weight after adding FBS and DMSO to determine if the final weight corresponds to the total volume.", "Gently open the draft shield door and remove the filled centrifuge tube.", "Close the draft shield door.", "Turn off the scales." ], "layout": { "id": "main", "name": "Main grid", "x": 0, "y": 0, "w": 40, "h": 40, "ueBlueprint": "", "relativeRotation": 0, "absoluteRotation": 0, "isSurface": true, "requiresSurface": false, "items": [ { "id": "Table_200_x_70_0", "name": "Table 200 x 70", "x": 0, "y": 1, "w": 40, "h": 14, "ueBlueprint": "/Game/Instruments/Tables/BP_Table_200x70.BP_Table_200x70_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "furniture/table_200x70.png", "thumbnailImage": "furniture/table_large.png", "isSurface": true, "requiresSurface": true, "items": [ { "id": "Dispenser_Paper_Table_0", "name": "Dispenser Paper Table", "x": 2, "y": 1, "w": 4, "h": 4, "ueBlueprint": "/Game/Instruments/Sanitising/BP_Dispenser_Paper_Towel.BP_Dispenser_Paper_Towel_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "cleaning/dispenser_paper_table.png", "thumbnailImage": "cleaning/dispenser_paper_table.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Wipes_IPA_0", "name": "Wipes IPA", "x": 2, "y": 6, "w": 5, "h": 3, "ueBlueprint": "/Game/Instruments/Sanitising/BP_Dispenser_Wipes.BP_Dispenser_Wipes_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "cleaning/wipes_ipa.png", "thumbnailImage": "cleaning/wipes_ipa.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Ethanol_Spray_Bottle_0", "name": "Ethanol Spray Bottle", "x": 3, "y": 10, "w": 3, "h": 2, "ueBlueprint": "/Game/Instruments/Sanitising/BP_Spray.BP_Spray_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "cleaning/spray_bottle.png", "thumbnailImage": "cleaning/spray_bottle.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Analytical_Scale_0", "name": "Analytical Scale", "x": 9, "y": 1, "w": 7, "h": 7, "ueBlueprint": "/Game/Instruments/Measuring/WeighingScale/BP_Analytical_Scale.BP_Analytical_Scale_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/analytical_scale.png", "thumbnailImage": "devices/analytical_scale.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Analytical_Scale_-_Calibration_Weights_0", "name": "Analytical Scale - 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