{ "version": "v3", "compatibility": "0.1.3", "collections": [ { "name": "Default (7 x SOPs)", "sops": [ { "name": "Eclipse creator tutorial", "steps": [ "Hand Tracking - Hold hands directly infront of face. They should be overlayed with purple gloves", "SOP steps UI - Tap tick to move forwards through the SOP steps and back arrow to move backwards. You can move the SOP UI by grabbing at the highlighted edges and dragging into position", "Dispensers - The small white boxes are dispensers with unlimited supply of consumables. If you hover your hand over them and grab or pinch, you will pick up the item contained. Have a practice at doing this", "Grabbing objects - Grab larger assets like bottles and bags etc to move them", "Heavy objects - Grab 'heavy' devices like the bioreactor vessel with 1 hand at the base to move from place to place.", "Pinching - Practice picking up small and thin objects like tubing and connectors. These are found in dispensers. Try using a pinching action.", "Tubing - The tubing can be picked up in the centre or manipulated at the ends (hover over to see blue orb). The tubing can also be stretched to reach attachment points.", "Snapslots - When inserting or connecting, snapslots will highlight to show the attachment can take place. Some objects may automatically snap while you may need to release others for them to position correctly. Try attaching the biobag to the bag stand and try placing the centrifuge tube on the vortex.", "Connecting - When connecting tubing or wires to a connector, filter or cap, pinch them by one end and pull to where or what you need to connect to. Still holding the tube end, attach connectors by positioning next to the tubing attachment point and releasing once the snapslot highlight appears.", "Pipette attachment - Pipette tips attach to micropipettes the same as they would in the lab. Remove the 1000ul micropipette from the stand and stab at the corresponding tip box. A tip should attach. To remove, hover the tip end inside of a bin.", "Pipetting - Similarly all pipetting assets including controllers and micropipettes interactions work the same as in the lab, using the relevant digits to withdraw and expel liquid. You can use the controller interfaces to help you with this.", "Opening/closing - Most devices and assets will open and close if you tap them at the opening/handle. For instance, try removing the cap from the bottle of distilled water by tapping it. ", "Device screens & UIs - Try using the touch screens on a device by tapping with the end of your index finger.", "Disposal - Dispose of consumables or waste into bins by holding them inside until you see the object outline appear and then releasing.", "Pop-ups - when hovering over liquid containers or instruments, a pop-up may appear. This can give details of the apparatus name or even the contents/features. These may be useful when creating solutions." ], "layout": { "id": "main", "name": "Main grid", "x": 0, "y": 0, "w": 40, "h": 40, "ueBlueprint": "", "relativeRotation": 0, "absoluteRotation": 0, "isSurface": true, "requiresSurface": false, "items": [ { "id": "Table_180_x_60_0", "name": "Table 180 x 60", "x": 0, "y": 0, "w": 36, "h": 12, "ueBlueprint": "/Game/Instruments/Tables/BP_Table_180x60.BP_Table_180x60_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "furniture/table_180x60.png", "thumbnailImage": "furniture/table_large.png", "isSurface": true, "requiresSurface": true, "items": [ { "id": "Stirred_Tank_Bioreactor_-_Vessel_0", "name": "Stirred Tank Bioreactor - Vessel", "x": 20, "y": 1, "w": 6, "h": 5, "ueBlueprint": "/Game/Instruments/Bioreactors/StirredTankBioreactor/BP_Stirred_Tank_Bioreactor.BP_Stirred_Tank_Bioreactor_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/univessel.png", "thumbnailImage": "devices/univessel.png", "isSurface": false, "requiresSurface": true, "items": [], "parts": [ { "slot": "TemperatureProbeSnap", "ueBlueprint": "/Game/Instruments/Bioreactors/StirredTankBioreactor/BP_Cap_Bioreactor_Probe_Insert.BP_Cap_Bioreactor_Probe_Insert_C", "parts": [] }, { "slot": "PHProbeSnap", "ueBlueprint": "/Game/Instruments/Bioreactors/StirredTankBioreactor/BP_Cap_Bioreactor_Probe_Insert.BP_Cap_Bioreactor_Probe_Insert_C", "parts": [] }, { "slot": "OxygenSnap", "ueBlueprint": "/Game/Instruments/Bioreactors/StirredTankBioreactor/BP_Cap_Bioreactor_Probe_Insert.BP_Cap_Bioreactor_Probe_Insert_C", "parts": [] }, { "slot": "AntifoamSnap", "ueBlueprint": "/Game/Instruments/Bioreactors/StirredTankBioreactor/BP_Cap_Bioreactor_Probe_Insert.BP_Cap_Bioreactor_Probe_Insert_C", "parts": [] } ] }, { "id": "Vortex_0", "name": "Vortex", "x": 15, "y": 1, "w": 5, "h": 5, "ueBlueprint": "/Game/Instruments/Vortex/BP_Vortex.BP_Vortex_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/vortex.png", "thumbnailImage": "devices/vortex.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Mini_Centrifuge_0", "name": "Mini Centrifuge", "x": 11, "y": 1, "w": 4, "h": 5, "ueBlueprint": "/Game/Instruments/Centrifuge/MiniCentrifuge/BP_Mini_Centrifuge.BP_Mini_Centrifuge_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/mini_centrifuge.png", "thumbnailImage": "devices/mini_centrifuge.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Pipettes_Mechanical_10ul,_25ul,_100ul,_1000ul_0", "name": "Pipettes Mechanical 10ul, 25ul, 100ul, 1000ul", "x": 5, "y": 1, "w": 5, "h": 3, "ueBlueprint": "/Game/Instruments/Pipettes/BP_Mechanical_Pipette_Stand.BP_Mechanical_Pipette_Stand_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/mechanical_pipette_rack.png", "thumbnailImage": "devices/mechanical_pipette_rack.png", "isSurface": false, "requiresSurface": true, "parts": [ { "slot": "Pipette10Snap", "ueBlueprint": "/Game/Instruments/Pipettes/BP_Mechanical_Pipette_Controller_10.BP_Mechanical_Pipette_Controller_10_C" }, { "slot": "Pipette20Snap", "ueBlueprint": "/Game/Instruments/Pipettes/BP_Mechanical_Pipette_Controller_20.BP_Mechanical_Pipette_Controller_20_C" }, { "slot": "Pipette200Snap", "ueBlueprint": "/Game/Instruments/Pipettes/BP_Mechanical_Pipette_Controller_200.BP_Mechanical_Pipette_Controller_200_C" }, { "slot": "Pipette1000Snap", "ueBlueprint": "/Game/Instruments/Pipettes/BP_Mechanical_Pipette_Controller_1000.BP_Mechanical_Pipette_Controller_1000_C" } ], "items": [] }, { "id": "Bin_Sharps_0", "name": "Bin Sharps", "x": 32, "y": 0, "w": 4, "h": 4, "ueBlueprint": "/Game/Instruments/Bins/BP_Bin_Sharps.BP_Bin_Sharps_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "cleaning/bin_sharps.png", "thumbnailImage": "cleaning/bin_sharps.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Bag_Stand_50cm_0", "name": "Bag Stand 50cm", "x": 27, "y": 0, "w": 4, "h": 4, "ueBlueprint": "/Game/Instruments/BagStands/BP_Bag_Stand_50cm.BP_Bag_Stand_50cm_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "bags/bag_stand_50cm.png", "thumbnailImage": "bags/bag_stand_50cm.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Distilled_Water_1L_0", "name": "Distilled Water 1L", "x": 32, "y": 5, "w": 3, "h": 3, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Distilled_Water_1L.BP_Bottle_Distilled_Water_1L_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle01_1l.png", "thumbnailImage": "containers/bottle1l_distilled_water.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Ethanol_Spray_Bottle_0", "name": "Ethanol Spray Bottle", "x": 1, "y": 1, "w": 3, "h": 2, "ueBlueprint": "/Game/Instruments/Sanitising/BP_Spray.BP_Spray_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "cleaning/spray_bottle.png", "thumbnailImage": "cleaning/spray_bottle.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Pipette_Controller_0", "name": "Pipette Controller", "x": 2, "y": 5, "w": 4, "h": 1, "ueBlueprint": "/Game/Instruments/Pipettes/BP_Pipette_Controller.BP_Pipette_Controller_C", "absoluteRotation": 90, "relativeRotation": 90, "gridImage": "devices/pipette_controller.png", "thumbnailImage": "devices/pipette_controller.png", "isSurface": false, "requiresSurface": false, "items": [] }, { "id": "Biofluid_Bag_3L_0", "name": "Biofluid Bag 3L", "x": 27, "y": 5, "w": 3, "h": 7, "ueBlueprint": "/Game/Instruments/Containers/Bags/BP_Fluid_Bag_BioFluid_3L.BP_Fluid_Bag_BioFluid_3L_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "bags/biofluid_bag_3l.png", "thumbnailImage": "bags/biofluid_bag_3l.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Pipette_tip_box_1000ul_0", "name": "Pipette tip box 1000ul", "x": 7, "y": 5, "w": 3, "h": 3, "ueBlueprint": "/Game/Instruments/Pipettes/Dispensers/BP_Dispenser_Micro_Pipettes_1000.BP_Dispenser_Micro_Pipettes_1000_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/dispenser_pipette_tips_1000ul.png", "thumbnailImage": "containers/dispenser_pipette_tips_1000ul.png", "isSurface": false, "requiresSurface": true, "items": [] } ] }, { "id": "Bio_Bin_6L_0", "name": "Bio Bin 6L", "x": 32, "y": 14, "w": 4, "h": 5, "ueBlueprint": "/Game/Instruments/Bins/BP_Bin_6L_Bio.BP_Bin_6L_Bio_C", "absoluteRotation": 90, "relativeRotation": 90, "gridImage": "cleaning/bio_bin_6l.png", "thumbnailImage": "cleaning/bio_bin_6l.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Table_120_x_45_0", "name": "Table 120 x 45", "x": 12, "y": 31, "w": 24, "h": 9, "ueBlueprint": "/Game/Instruments/Tables/BP_Table_Stand_Small_Thin.BP_Table_Stand_Small_Thin_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "furniture/table_thin.png", "thumbnailImage": "furniture/table_thin.png", "isSurface": true, "requiresSurface": true, "items": [ { "id": "Centrifuge_Tube_50ml_0", "name": "Centrifuge Tube 50ml", "x": 19, "y": 5, "w": 4, "h": 3, "ueBlueprint": "/Game/Instruments/Centrifuge/BP_Dispenser_Centrifuge_Tube_50ml.BP_Dispenser_Centrifuge_Tube_50ml_C", "absoluteRotation": 180, "relativeRotation": 180, "gridImage": "containers/dispenser_centrifuge_tube_50ml.png", "thumbnailImage": "containers/centrifuge_tube_50ml.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Microcentrifuge_Tube_1.5ml_0", "name": "Microcentrifuge Tube 1.5ml", "x": 20, "y": 2, "w": 3, "h": 2, "ueBlueprint": "/Game/Instruments/Centrifuge/BP_Dispenser_Microcentrifuge_Tube_1halfml.BP_Dispenser_Microcentrifuge_Tube_1halfml_C", "absoluteRotation": 180, "relativeRotation": 180, "gridImage": "containers/dispenser_microcentrifuge_tube_one_half_ml.png", "thumbnailImage": "containers/microcentrifuge_tube_one_half_ml.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Seropipette_10ml_0", "name": "Seropipette 10ml", "x": 10, "y": 7, "w": 8, "h": 1, "ueBlueprint": "/Game/Instruments/Pipettes/Dispensers/BP_Dispenser_Seropips_10ml.BP_Dispenser_Seropips_10ml_C", "absoluteRotation": 180, "relativeRotation": 180, "gridImage": "containers/dispenser_seropipette_10ml.png", "thumbnailImage": "containers/seropip_10ml.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Tubing_Generic_0", "name": "Tubing Generic", "x": 10, "y": 4, "w": 8, "h": 2, "ueBlueprint": "/Game/Instruments/Tubing/Dispensers/BP_Dispenser_Tubing.BP_Dispenser_Tubing_C", "absoluteRotation": 180, "relativeRotation": 180, "gridImage": "tubing/dispenser_tubing.png", "thumbnailImage": "tubing/tubing_generic.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Sterile_Filter_0", "name": "Sterile Filter", "x": 16, "y": 1, "w": 3, "h": 2, "ueBlueprint": "/Game/Instruments/Tubing/Dispensers/BP_Dispenser_Connector_Sterile_Filter.BP_Dispenser_Connector_Sterile_Filter_C", "absoluteRotation": 180, "relativeRotation": 180, "gridImage": "tubing/dispenser_sterile_filter.png", "thumbnailImage": "tubing/sterile_filter.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Tube_Connector_Y_0", "name": "Tube Connector Y", "x": 13, "y": 2, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Tubing/Dispensers/BP_Dispenser_Connector_Y.BP_Dispenser_Connector_Y_C", "absoluteRotation": 180, "relativeRotation": 180, "gridImage": "tubing/dispenser_tube_y.png", "thumbnailImage": "tubing/tube_y.png", "isSurface": false, "requiresSurface": true, "items": [] } ] } ], "thumbnailImage": "", "gridImage": "", "parts": [] } }, { "name": "Precision weighing and measuring SOP ", "steps": [ "Purpose and Scope: Initial training into the calibration of a weighing scale prior to weighing liquids for accuracy post pipetting. ", "Check for visible damage, loose components, or debris on the weighing platform.", "Ensure the draft shield door on the scales is closed. ", "Turn on the analytical scale and allow it to stabilise.", "Check the scale is balanced by inspecting the indicator. The bubble should be in the centre of the indicator ring. If not this can be adjusted by tapping each wheel. ", "Check that the measurement mode is in grams.", "Press the Function then Tare button to access to the calibration screen.", "Gently open the draft field door.", "Pick up the smallest/lightest calibration weight from the box and place in the centre of the scale. ", "Gently close the draft field door by touching the handle.", "The scale should show weight but wait a few seconds until balance settles on a weight.", "Make a note of the weight in a calibration book.", "Gently open the draft field door by touching the handle.", "Remove the weight from the scale and close the draft field door.", "Re-TARE the scale.", "Repeat the calibration/calibration weight weighing process with the other weights in order lightest-heaviest.", "Once all the weights have been calibrated , double tap the function button to take you back to the sample measuring mode.", "Press the TARE button. ", "Gently open the draft field door. ", "Place the empty 50ml centrifuge tube on the scale and gently close the draft field door. ", "Make a note of the weight pre-liquid. ", "Press TARE. ", "Gently open the draft field door and remove the empty centrifuge tube. ", "Gently close the draft field door. ", "Using a serological pipette and pipette controller, transfer 36ml FBS into the centrifuge tube. ", "Gently open the draft field door. ", "Place the filled 50ml centrifuge tube on the scale and gently close the draft field door. ", "Make a note of the weight post FBS. ", "Gently open the draft field door and remove the filled centrifuge tube. ", "Gently close the draft field door.", "Using a 1000ul mechanical pipette and 1000ul tips, transfer 4ml of DMSO 1ml by 1ml pipetting up and down each time to mix, into the centrifuge tube to bring the total volume to 40ml. ", "Gently open the draft field door.", "Place the filled 50ml centrifuge tube on the scale and gently close the draft field door.", "Make a note of the weight post FBS&DMSO to determine if the final weight corresponds to the total volume.", "Gently open the draft field door and remove the filled centrifuge tube.", "Close the draft field door.", "Turn off the scales." ], "layout": { "id": "main", "name": "Main grid", "x": 0, "y": 0, "w": 40, "h": 40, "ueBlueprint": "", "relativeRotation": 0, "absoluteRotation": 0, "isSurface": true, "requiresSurface": false, "items": [ { "id": "Table_200_x_70_0", "name": "Table 200 x 70", "x": 0, "y": 1, "w": 40, "h": 14, "ueBlueprint": "/Game/Instruments/Tables/BP_Table_200x70.BP_Table_200x70_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "furniture/table_200x70.png", "thumbnailImage": "furniture/table_large.png", "isSurface": true, "requiresSurface": true, "items": [ { "id": "Dispenser_Paper_Table_0", "name": "Dispenser Paper Table", "x": 2, "y": 1, "w": 4, "h": 4, "ueBlueprint": "/Game/Instruments/Sanitising/BP_Dispenser_Paper_Towel.BP_Dispenser_Paper_Towel_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "cleaning/dispenser_paper_table.png", "thumbnailImage": "cleaning/dispenser_paper_table.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Wipes_IPA_0", "name": "Wipes IPA", "x": 2, "y": 6, "w": 5, "h": 3, "ueBlueprint": "/Game/Instruments/Sanitising/BP_Dispenser_Wipes.BP_Dispenser_Wipes_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "cleaning/wipes_ipa.png", "thumbnailImage": "cleaning/wipes_ipa.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Ethanol_Spray_Bottle_0", "name": "Ethanol Spray Bottle", "x": 3, "y": 10, "w": 3, "h": 2, "ueBlueprint": "/Game/Instruments/Sanitising/BP_Spray.BP_Spray_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "cleaning/spray_bottle.png", "thumbnailImage": "cleaning/spray_bottle.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Analytical_Scale_0", "name": "Analytical Scale", "x": 9, "y": 1, "w": 7, "h": 7, "ueBlueprint": "/Game/Instruments/Measuring/WeighingScale/BP_Analytical_Scale.BP_Analytical_Scale_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/analytical_scale.png", "thumbnailImage": "devices/analytical_scale.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Analytical_Scale_-_Calibration_Weights_0", "name": "Analytical Scale - Calibration Weights", "x": 17, "y": 1, "w": 6, "h": 2, "ueBlueprint": "/Game/Instruments/Measuring/WeighingScale/CalibrationWeights/BP_Calibration_Weights_Box.BP_Calibration_Weights_Box_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/analytical_scale_weights.png", "thumbnailImage": "devices/analytical_scale_weights.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Pipette_tip_box_1000ul_0", "name": "Pipette tip box 1000ul", "x": 24, "y": 1, "w": 3, "h": 3, "ueBlueprint": "/Game/Instruments/Pipettes/Dispensers/BP_Dispenser_Micro_Pipettes_1000.BP_Dispenser_Micro_Pipettes_1000_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/dispenser_pipette_tips_1000ul.png", "thumbnailImage": "containers/dispenser_pipette_tips_1000ul.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Pipette_Controller_0", "name": "Pipette Controller", "x": 31, "y": 5, "w": 1, "h": 4, "ueBlueprint": "/Game/Instruments/Pipettes/BP_Pipette_Controller.BP_Pipette_Controller_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/pipette_controller.png", "thumbnailImage": "devices/pipette_controller.png", "isSurface": false, "requiresSurface": false, "items": [] }, { "id": "Bin_Sharps_0", "name": "Bin Sharps", "x": 35, "y": 1, "w": 4, "h": 4, "ueBlueprint": "/Game/Instruments/Bins/BP_Bin_Sharps.BP_Bin_Sharps_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "cleaning/bin_sharps.png", "thumbnailImage": "cleaning/bin_sharps.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Pipettes_Mechanical_10ul,_25ul,_100ul,_1000ul_0", "name": "Pipettes Mechanical 10ul, 25ul, 100ul, 1000ul", "x": 28, "y": 1, "w": 5, "h": 3, "ueBlueprint": "/Game/Instruments/Pipettes/BP_Mechanical_Pipette_Stand.BP_Mechanical_Pipette_Stand_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/mechanical_pipette_rack.png", "thumbnailImage": "devices/mechanical_pipette_rack.png", "isSurface": false, "requiresSurface": true, "parts": [ { "slot": "Pipette10Snap", "ueBlueprint": "/Game/Instruments/Pipettes/BP_Mechanical_Pipette_Controller_10.BP_Mechanical_Pipette_Controller_10_C" }, { "slot": "Pipette20Snap", "ueBlueprint": "/Game/Instruments/Pipettes/BP_Mechanical_Pipette_Controller_20.BP_Mechanical_Pipette_Controller_20_C" }, { "slot": "Pipette200Snap", "ueBlueprint": "/Game/Instruments/Pipettes/BP_Mechanical_Pipette_Controller_200.BP_Mechanical_Pipette_Controller_200_C" }, { "slot": "Pipette1000Snap", "ueBlueprint": "/Game/Instruments/Pipettes/BP_Mechanical_Pipette_Controller_1000.BP_Mechanical_Pipette_Controller_1000_C" } ], "items": [] }, { "id": "Fetal_Bovine_Serum_(FBS)_1L_0", "name": "Fetal Bovine Serum (FBS) 1L", "x": 20, "y": 6, "w": 3, "h": 3, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_FBS_1L.BP_Bottle_FBS_1L_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle02_1l.png", "thumbnailImage": "containers/bottle1l_fbs.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "DMSO_100ml_0", "name": "DMSO 100ml", "x": 25, "y": 7, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_DMSO_100ml.BP_Bottle_DMSO_100ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle02_100ml.png", "thumbnailImage": "containers/bottle100ml_dmso.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Centrifuge_Tube_50ml_0", "name": "Centrifuge Tube 50ml", "x": 34, "y": 6, "w": 4, "h": 3, "ueBlueprint": "/Game/Instruments/Centrifuge/BP_Dispenser_Centrifuge_Tube_50ml.BP_Dispenser_Centrifuge_Tube_50ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/dispenser_centrifuge_tube_50ml.png", "thumbnailImage": "containers/centrifuge_tube_50ml.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Seropipette_25ml_0", "name": "Seropipette 25ml", "x": 30, "y": 10, "w": 8, "h": 1, "ueBlueprint": "/Game/Instruments/Pipettes/Dispensers/BP_Dispenser_Seropips_25ml.BP_Dispenser_Seropips_25ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/dispenser_seropipette_25ml.png", "thumbnailImage": "containers/seropip_25ml.png", "isSurface": false, "requiresSurface": true, "items": [] } ] }, { "id": "Bio_Bin_6L_0", "name": "Bio Bin 6L", "x": 35, "y": 16, "w": 5, "h": 4, "ueBlueprint": "/Game/Instruments/Bins/BP_Bin_6L_Bio.BP_Bin_6L_Bio_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "cleaning/bio_bin_6l.png", "thumbnailImage": "cleaning/bio_bin_6l.png", "isSurface": false, "requiresSurface": true, "items": [] } ], "thumbnailImage": "", "gridImage": "", "parts": [] } }, { "name": "Pipetting", "steps": [ "Purpose and scope: Pipetting is used for transferring solutions between locations. It is also a common technique used in cell culture. For the purposes of this training you will learn 3 types of pipetting: Serological pipetting with large volumes of liquid; Micro-pipetting with small volumes of liquid and Multichannel pipetting for more efficient micro-pipetting.", "Turn on Microbiological/Biological safety cabinet (MSC/BSC).", "Turn on MSC light.", "Turn on laminar airflow (fan).", "Use buttons to lift MSC glass sash.", "Spray hands with ethanol spray before entering MSC.", "Retrieve 1x 200ml centrifuge tube from dispenser.", "Open media bottle and 200ml centrifuge tube.", "Retrieve 1x100ml serological pipette from dispenser box and attach to the end of the pipette controller. ", "Using the correct pipetting action (index finger in and middle finger out to withdraw and middle finger in but index finger out to expel), withdraw 100ml of DMEM from bottle and expel into empty 200ml centrifuge tube. ", "Remove waste serological pipette and place in biobin. ", "Retrieve a 10ml serological pipette from dispenser box and attach to the end of the pipette controller. ", "Withdraw 10ml DMEM from the DMEM bottle and expel into the same 200ml centrifuge tube. ", "Remove waste serological pipette and place in biobin. ", "Place the pipette controller to the side of the working surface.", "Obtain a 1ml pipette from the pipette rack and insert it into the 1000µl pipette tip box to attach a tip. ", "Ensuring the pipette is set at 1000ul, withdraw 1ml DMEM from the DMEM bottle and expel into the same 200ml centrifuge tube. ", "Remove the waste pipette tip by ejecting into the sharps bin and return 1ml micropipette to stand. ", "Close lid of 200ml waste centrifuge and place to left side of cabinet.", "Open lid of bottle of FBS.", "Retrieve a 100ml serological pipette from dispenser box and attach to the end of the pipette controller.", "Withdraw 100ml FBS from FBS bottle and expel into DMEM bottle. ", "Mix by flushing DMEM+FBS mixture up and down in the serological pipette. ", "Close FBS bottle lid. ", "Remove waste serological pipette and place in biobin. ", "Open bottle of Ultra Glutamine.", "Retrieve 10ml serological pipette from dispenser box and attach to the end of the pipette controller. ", "Withdraw 10ml Ultra Glutamine and expel into DMEM bottle. ", "Mix by flushing DMEM+FBS+Ultra Glutamine mixture up and down in the serological pipette. ", "Close Ultra Glutamine bottle lid. ", "Open aliquot of bFGF.", "Obtain a 1ml pipette from the pipette rack and insert it into the 1000µl pipette tip box to attach a tip. ", "Withdraw 1ml bFGF and expel into DMEM bottle. ", "Close aliquot of bFGF.", "Dispose of pipette tip in the biobin.", "Retrieve 100ml serological pipette from dispenser box and attach to the end of the pipette controller. ", "Mix complete culture medium by flushing DMEM+FBS+Ultra Glutamine+bFGF mixture up and down using a serological pipette. ", "Expel 100ml of this complete culture media into a sterile trough using a serological pipette. ", "Dispose of waste serological pipette and close complete media bottle. ", "Retrieve a 96-well plate. ", "Attach multichannel pipette tips to the multichannel pipette and withdraw complete cell culture media from the trough into the tips using the correct pipetting motion. ", "Expel the media from these tips and into the 96 well plate row by row until all rows are filled with complete media. ", "Once filled, dispose of waste pipette tips and trough. " ], "layout": { "id": "main", "name": "Main grid", "x": 0, "y": 0, "w": 40, "h": 40, "ueBlueprint": "", "relativeRotation": 0, "absoluteRotation": 0, "isSurface": true, "requiresSurface": false, "items": [ { "id": "Table_60_x_45_0", "name": "Table 60 x 45", "x": 30, "y": 17, "w": 9, "h": 12, "ueBlueprint": "/Game/Instruments/Tables/BP_Table_Stand_Small.BP_Table_Stand_Small_C", "absoluteRotation": 90, "relativeRotation": 90, "gridImage": "furniture/table_stand.png", "thumbnailImage": "furniture/table_stand.png", "isSurface": true, "requiresSurface": true, "items": [ { "id": "Ethanol_Spray_Bottle_0", "name": "Ethanol Spray Bottle", "x": 1, "y": 1, "w": 3, "h": 2, "ueBlueprint": "/Game/Instruments/Sanitising/BP_Spray.BP_Spray_C", "absoluteRotation": 0, "relativeRotation": 270, "gridImage": "cleaning/spray_bottle.png", "thumbnailImage": "cleaning/spray_bottle.png", "isSurface": false, "requiresSurface": true, "items": [] } ] }, { "id": "MSC_Large_0", "name": "MSC Large", "x": 0, "y": 0, 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{ "id": "bFGF_0", "name": "bFGF", "x": 27, "y": 6, "w": 3, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Dispensers/BP_Dispenser_Vial_bFGF.BP_Dispenser_Vial_bFGF_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/dispenser_bfgf.png", "thumbnailImage": "containers/bfgf.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Pipette_tip_box_1000ul_0", "name": "Pipette tip box 1000ul", "x": 21, "y": 2, "w": 3, "h": 3, "ueBlueprint": "/Game/Instruments/Pipettes/Dispensers/BP_Dispenser_Micro_Pipettes_1000.BP_Dispenser_Micro_Pipettes_1000_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/dispenser_pipette_tips_1000ul.png", "thumbnailImage": "containers/dispenser_pipette_tips_1000ul.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Multichannel_pipette_tip_box_0", "name": "Multichannel pipette tip box", "x": 17, "y": 2, "w": 3, "h": 3, "ueBlueprint": 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"/Game/Instruments/Containers/WellPlates/BP_Dispener_Well_Plate_96.BP_Dispener_Well_Plate_96_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/dispenser_well_plate_96.png", "thumbnailImage": "containers/well_plate_96.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Centrifuge_Tube_200ml_0", "name": "Centrifuge Tube 200ml", "x": 7, "y": 2, "w": 4, "h": 3, "ueBlueprint": "/Game/Instruments/Centrifuge/BP_Dispenser_Centrifuge_Tube_200ml.BP_Dispenser_Centrifuge_Tube_200ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/dispenser_centrifuge_tube_200ml.png", "thumbnailImage": "containers/centrifuge_tube_200ml.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Bin_Sharps_0", "name": "Bin Sharps", "x": 2, "y": 2, "w": 4, "h": 4, "ueBlueprint": "/Game/Instruments/Bins/BP_Bin_Sharps.BP_Bin_Sharps_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "cleaning/bin_sharps.png", "thumbnailImage": "cleaning/bin_sharps.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "T25_Flask_0", "name": "T25 Flask", "x": 11, "y": 6, "w": 4, "h": 3, "ueBlueprint": "/Game/Instruments/Containers/TFlask/BP_Dispenser_T25_Flask.BP_Dispenser_T25_Flask_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/dispenser_t25.png", "thumbnailImage": "containers/flask_horizontal.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Seropipette_100ml_0", "name": "Seropipette 100ml", "x": 2, "y": 6, "w": 8, "h": 2, "ueBlueprint": "/Game/Instruments/Pipettes/Dispensers/BP_Dispenser_Seropips_100ml.BP_Dispenser_Seropips_100ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/dispenser_seropipette_100ml.png", "thumbnailImage": "containers/seropip_100ml.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Seropipette_10ml_0", "name": "Seropipette 10ml", "x": 2, "y": 8, "w": 8, "h": 1, "ueBlueprint": "/Game/Instruments/Pipettes/Dispensers/BP_Dispenser_Seropips_10ml.BP_Dispenser_Seropips_10ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/dispenser_seropipette_10ml.png", "thumbnailImage": "containers/seropip_10ml.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Trough_0", "name": "Trough", "x": 2, "y": 10, "w": 4, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Troughs/BP_Dispenser_Trough.BP_Dispenser_Trough_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/dispenser_trough.png", "thumbnailImage": "containers/trough.png", "isSurface": false, "requiresSurface": true, "items": [] } ] }, { "id": "Bio_Bin_6L_0", "name": "Bio Bin 6L", "x": 26, "y": 25, "w": 4, "h": 5, "ueBlueprint": "/Game/Instruments/Bins/BP_Bin_6L_Bio.BP_Bin_6L_Bio_C", "absoluteRotation": 90, "relativeRotation": 90, "gridImage": "cleaning/bio_bin_6l.png", "thumbnailImage": "cleaning/bio_bin_6l.png", "isSurface": false, "requiresSurface": true, "items": [] } ], "thumbnailImage": "", "gridImage": "", "parts": [] } }, { "name": "Peristaltic pump SOP", "steps": [ "This document describes the operation of a peristaltic pump for the purpose of filling Biobags with cell culture Media under sterile conditions. ", "Turn on Microbiological safety cabinet (MSC) by pressing the power button on the MSC’s touch display. ", "On the MSC’s touch display, turn on MSC light (lightbulb button).", "On the MSC’s touch display, turn on laminar airflow (fan).", "On the MSC’s touch display, use the upwards arrow button to lift MSC glass sash. Wait until the sash is fully open before continuing.", "Grab the ethanol spray bottle located on the table opposite the MSC and liberally spray both hands to sanitise them before working in the MSC.", "Attach biobag (1L) to bag stand.", "Retrieve a sterile filter from the dispenser box.", "Retrieve sterile tubing from the dispenser box.", "Attach a sterile filter to one end of the tubing and attach this end to the biobag on the bag stand .", "Remove the lid from the bottle of General culture medium", "Retrieve a sterile fluid transfer cap from the dispenser box. ", "Attach the sterile fluid transfer cap to the bottle of medium. ", "Insert the other end of the tubing to the media sterile fluid transfer cap tubing.", "On the front of the peristaltic pump, press the modulus to open.", "Place the centre of the tube into the pump modulus.", "Retrieve pinch clamps from the pinch clamp dispenser and attach at the tubing ends closest to the biofluid bag and closest to the fluid transfer cap.", "Tap open on the pinch clamps to open them both.", "Adjust the speed to 3500ml/min on the pump using the up and down arrows .", "Press the start button to begin pumping media from the bottle to the bag.", "Once the bag is full and bottle empty, press the start/pause button again to stop the pumping.", "Close the pinch clamps.", "Open modulus and remove the tubing.", "Remove the lid from sterile water bottle. ", "Retrieve a sterile fluid transfer cap from the dispenser box.  ", "Attach the sterile fluid transfer cap to the bottle of sterile water. ", "Open the lid of the empty waste bottle", "Retrieve a sterile fluid transfer cap from the dispenser box.  ", "Attach the sterile fluid transfer cap to the empty waste bottle. ", "Remove tubing from the bag, leaving the sterile filter, and remove tubing from the empty media bottle, leaving the sterile fluid transfer cap. ", "Insert the bottle end of the tubing to the sterile water sterile fluid transfer cap tubing.", "Insert the bag end of the tubing to the waste bottle sterile fluid transfer cap.", "Discard of the cell culture media bottle and used fluid transfer cap. ", "Spray hands with ethanol before re-entering the MSC.", "The sterile filter should remain attached to the biobag and both left as they are. The biobag is now full of cell culture media ready for the inoculation of the bioreactor vessel.", "Open the pump modulus and place the centre of the tube inside it.", "Open the pinch clamps.", "Press the start button to begin pumping water from the bottle to the waste bottle, diluting out and washing away culture medium. ", "Once the waste bottle is full and sterile water container empty, press the start/pause button again to stop the pumping.", "You have now rinsed the tubing of cell culture media and have an uncontaminated system ready for pumping fresh biofluids." ], "layout": { "id": "main", "name": "Main grid", "x": 0, "y": 0, "w": 40, "h": 40, "ueBlueprint": "", "relativeRotation": 0, "absoluteRotation": 0, "isSurface": true, "requiresSurface": false, "items": [ { "id": "MSC_Large_0", "name": "MSC Large", "x": 0, "y": 0, "w": 36, "h": 17, "ueBlueprint": "/Game/Instruments/MSC/BP_MSC_Herasafe_Standing_Large.BP_MSC_Herasafe_Standing_Large_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/msc_large.png", "thumbnailImage": "devices/msc_large.png", "isSurface": true, "requiresSurface": false, "items": [ { "id": "Tubing_Generic_0", "name": "Tubing Generic", "x": 2, "y": 2, "w": 8, "h": 2, "ueBlueprint": 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"tubing/dispenser_fluid_transfer_cap.png", "thumbnailImage": "tubing/fluid_transfer_cap.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Peristaltic_Pump_Digital_0", "name": "Peristaltic Pump Digital", "x": 18, "y": 2, "w": 5, "h": 9, "ueBlueprint": "/Game/Instruments/LiquidPumps/PeristalticPump/BP_Peristaltic_Pump.BP_Peristaltic_Pump_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/peristaltic_pump.png", "thumbnailImage": "devices/peristaltic_pump.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Bag_Stand_50cm_0", "name": "Bag Stand 50cm", "x": 25, "y": 2, "w": 4, "h": 4, "ueBlueprint": "/Game/Instruments/BagStands/BP_Bag_Stand_50cm.BP_Bag_Stand_50cm_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "bags/bag_stand_50cm.png", "thumbnailImage": "bags/bag_stand_50cm.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Biofluid_Bag_1L_0", "name": "Biofluid Bag 1L", "x": 25, "y": 7, "w": 4, "h": 5, "ueBlueprint": "/Game/Instruments/Containers/Bags/BP_Fluid_Bag_BioFluid_1L.BP_Fluid_Bag_BioFluid_1L_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "bags/biofluid_bag_1l.png", "thumbnailImage": "bags/biofluid_bag_1l.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Sterile_Water_1L_0", "name": "Sterile Water 1L", "x": 14, "y": 2, "w": 3, "h": 3, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Sterile_Water_1L.BP_Bottle_Sterile_Water_1L_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle01_1l.png", "thumbnailImage": "containers/bottle1l_sterile_water.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Pinch_Clamp_0", "name": "Pinch Clamp", "x": 8, "y": 8, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Tubing/Dispensers/BP_Dispenser_Connector_Pinch_Clamp.BP_Dispenser_Connector_Pinch_Clamp_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "tubing/dispenser_pinch_clamp.png", "thumbnailImage": "tubing/pinch_clamp.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Bottle_1L_0", "name": "Bottle 1L", "x": 31, "y": 2, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/GL45/BP_Bottle_GL45_1L.BP_Bottle_GL45_1L_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle03_1l.png", "thumbnailImage": "containers/bottle03_1l.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "General_Culture_Media_0", "name": "General Culture Media", "x": 14, "y": 6, "w": 3, "h": 3, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Cell_Culture_Medium_1L.BP_Bottle_Cell_Culture_Medium_1L_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle02_1l.png", "thumbnailImage": "containers/general_culture_media1L.png", "isSurface": false, "requiresSurface": true, "items": [] } ] }, { "id": "Bio_Bin_6L_0", "name": "Bio Bin 6L", "x": 26, "y": 25, "w": 4, "h": 5, "ueBlueprint": "/Game/Instruments/Bins/BP_Bin_6L_Bio.BP_Bin_6L_Bio_C", "absoluteRotation": 90, "relativeRotation": 90, "gridImage": "cleaning/bio_bin_6l.png", "thumbnailImage": "cleaning/bio_bin_6l.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Table_60_x_45_0", "name": "Table 60 x 45", "x": 31, "y": 21, "w": 9, "h": 12, "ueBlueprint": "/Game/Instruments/Tables/BP_Table_Stand_Small.BP_Table_Stand_Small_C", "absoluteRotation": 90, "relativeRotation": 90, "gridImage": "furniture/table_stand.png", "thumbnailImage": "furniture/table_stand.png", "isSurface": true, "requiresSurface": true, "items": [ { "id": "Ethanol_Spray_Bottle_0", "name": "Ethanol Spray Bottle", "x": 1, "y": 1, "w": 3, "h": 2, "ueBlueprint": "/Game/Instruments/Sanitising/BP_Spray.BP_Spray_C", "absoluteRotation": 0, "relativeRotation": 270, "gridImage": "cleaning/spray_bottle.png", "thumbnailImage": "cleaning/spray_bottle.png", "isSurface": false, "requiresSurface": true, "items": [] } ] } ], "thumbnailImage": "", "gridImage": "", "parts": [] } }, { "name": "Stirred tank bioreactor set up SOP", "steps": [ "Purpose and Scope: This document describes the set up and calibration of a stirred tank bioreactor vessel and controller.", "Turn on Microbiological safety cabinet (MSC) by pressing the power button on the MSC’s touch display. ", "On the MSC’s touch display, turn on MSC light.", "On the MSC’s touch display, turn on laminar airflow (fan).", "On the MSC’s touch display, use the upwards arrow button to lift MSC glass sash. Wait until the sash is fully open before continuing.", "Grab the ethanol spray bottle located on the table opposite the MSC and liberally spray both hands to sanitise them before working in the MSC.", "On the bioreactor headplate, there are four (4) probe ports with blank plugs. Remove each of these blank plugs and set them aside (in the MSC). Do so by pinching your forefinger and thumb as if lifting a small object.", "Pinch to pick up temperature probe and insert into the bioreactor vessel thermowell open sensor port. ", "Pinch to pick up dissolved oxygen sensor probe (aka dO2 probe) and pass it through the compatible bioreactor vessel open sensor port. ", "Pinch to pick up PH sensor probe and pass it through the compatible bioreactor vessel open sensor port. ", "Pinch to pick up antifoam sensor probe and pass it through the compatible bioreactor vessel open sensor port. ", "With one hand at the base, grab the assembled bioreactor and remove it from the MSC.", "Place the bioreactor into the bioreactor base platform on the benchtop.", "Connect a length of sterile tubing to the filter on the bioreactor sparger line (the sparger immersed pipe looks L-shaped and metallic, with a ring end running below the impellers).", "Grab the end of the sparger tubing and connect this to the sparge gas outlet on the side of the bioreactor control tower. This outlet is labelled as “Air In / Sparger”", "Pick up the motor base attachment and connect this to the top of the motor shaft on the bioreactor headplate.", "Pick up motor and attach it on top of the motor attachment unit. ", "Connect the temperature probe cable to the temperature port on the bioreactor control tower. This port is labelled “Temp”.", "Connect the dissolved oxygen sensor cable to the dissolved oxygen port on the bioreactor control tower. This port is labelled “pO2” (pO2 = partial pressure of oxygen).", "Connect the pH probe cable to the pH port on the bioreactor control tower. This is labelled “PH”.", "Connect the antifoam sensor cable to the antifoam sensor port on the bioreactor control tower. This is labelled “Antifoam”.", "Connect the motor power cord to the socket labelled “Motor” on the bioreactor control tower.", "With one hand, pick up the thermal heating jacket and wrap the bioreactor.", "Connect the heating jacket’s power cable to the socket on the bioreactor control tower, labelled “Heat”", "Connect up the bioreactor vessel base stand wire to the \"scale\" port on the control tower unit.", "Move to the bioreactor control tower touchscreen.", "On the bioreactor controller main touch screen, press the button labelled “Heat” and select auto (37C) in the sub-menu to turn on temperature control.", "On the bioreactor controller main touch screen, press the button labelled “AIR” and select “Auto” in the sub-menu (1.85 lpm)", "In the same way as step 27, set the settings for O2, N2 and CO2 to “auto” in their individual sub-menus. Respectively this should yield set-point flowrates of 1.00 lpm (O2), 0 lpm (N2) and 0.15 lpm (CO2) (lpm = litres per minute)", "Press the “STIR” button on the bioreactor controller main touch screen and select auto (80rpm) in the sub-menu to begin agitation of the bioreactor." ], "layout": { "id": "main", "name": "Main grid", "x": 0, "y": 0, "w": 40, "h": 40, "ueBlueprint": "", "relativeRotation": 0, "absoluteRotation": 0, "isSurface": true, "requiresSurface": false, "items": [ { "id": "Table_200_x_70_0", "name": "Table 200 x 70", "x": 0, "y": 26, "w": 40, "h": 14, "ueBlueprint": "/Game/Instruments/Tables/BP_Table_200x70.BP_Table_200x70_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "furniture/table_200x70.png", "thumbnailImage": "furniture/table_large.png", "isSurface": true, "requiresSurface": true, "items": [ { "id": "Stirred_Tank_Bioreactor_-_Motor_0", "name": "Stirred Tank Bioreactor - Motor", "x": 27, "y": 4, "w": 2, "h": 7, "ueBlueprint": "/Game/Instruments/Bioreactors/StirredTankBioreactor/BP_Motor_Stirred_Tank_Bioreactor.BP_Motor_Stirred_Tank_Bioreactor_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/univessel_motor.png", "thumbnailImage": "devices/univessel_motor.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Stirred_Tank_Bioreactor_-_Motor_Adapter_0", "name": "Stirred Tank Bioreactor - Motor Adapter", "x": 24, "y": 7, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Bioreactors/StirredTankBioreactor/BP_Motor_Base_Stirred_Tank_Bioreactor.BP_Motor_Base_Stirred_Tank_Bioreactor_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/univessel_motor_adapter.png", "thumbnailImage": "devices/univessel_motor_adapter.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Stirred_Tank_Bioreactor_-_Controller_0", "name": "Stirred Tank Bioreactor - Controller", "x": 1, "y": 1, "w": 8, "h": 11, "ueBlueprint": "/Game/Instruments/Biostat/BP_Biostat.BP_Biostat_C", "absoluteRotation": 180, "relativeRotation": 180, "gridImage": "devices/biostat_controller.png", "thumbnailImage": "devices/biostat_controller.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Stirred_Tank_Bioreactor_-_Thermal_Heating_Jacket_0", "name": "Stirred Tank Bioreactor - Thermal Heating Jacket", "x": 31, "y": 0, "w": 5, "h": 14, "ueBlueprint": "/Game/Instruments/Bioreactors/StirredTankBioreactor/BP_Thermal_Jacket.BP_Thermal_Jacket_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/heating_jacket.png", "thumbnailImage": "devices/heating_jacket.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Stirred_Tank_Bioreactor_-_Vessel_Base_0", "name": "Stirred Tank Bioreactor - Vessel Base", "x": 13, "y": 7, "w": 7, "h": 5, "ueBlueprint": "/Game/Instruments/Bioreactors/StirredTankBioreactor/BP_Base_Stirred_Tank_Bioreactor.BP_Base_Stirred_Tank_Bioreactor_C", "absoluteRotation": 270, "relativeRotation": 270, "gridImage": "devices/univessel_base.png", "thumbnailImage": "devices/univessel_base.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Tubing_Generic_0", "name": "Tubing Generic", "x": 15, "y": 2, "w": 8, "h": 2, "ueBlueprint": "/Game/Instruments/Tubing/Dispensers/BP_Dispenser_Tubing.BP_Dispenser_Tubing_C", "absoluteRotation": 180, "relativeRotation": 180, "gridImage": "tubing/dispenser_tubing.png", "thumbnailImage": "tubing/tubing_generic.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Ethanol_Spray_Bottle_0", "name": "Ethanol Spray Bottle", "x": 11, "y": 2, "w": 2, "h": 3, "ueBlueprint": "/Game/Instruments/Sanitising/BP_Spray.BP_Spray_C", "absoluteRotation": 90, "relativeRotation": 90, "gridImage": "cleaning/spray_bottle.png", "thumbnailImage": "cleaning/spray_bottle.png", "isSurface": false, "requiresSurface": true, "items": [] } ] }, { "id": "MSC_Small_0", "name": "MSC Small", "x": 0, "y": 0, "w": 28, "h": 13, "ueBlueprint": "/Game/Instruments/MSC/BP_MSC_Herasafe_Standing.BP_MSC_Herasafe_Standing_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/msc.png", "thumbnailImage": "devices/msc.png", "isSurface": true, "requiresSurface": false, "items": [ { "id": "Stirred_Tank_Bioreactor_-_Temperature_Probe_0", "name": "Stirred Tank Bioreactor - Temperature Probe", "x": 2, "y": 3, "w": 1, "h": 5, "ueBlueprint": "/Game/Instruments/Bioreactors/StirredTankBioreactor/BP_Temperature_Probe.BP_Temperature_Probe_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/temp_probe.png", "thumbnailImage": "devices/temp_probe.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Stirred_Tank_Bioreactor_-_PH_Probe_0", "name": "Stirred Tank Bioreactor - PH Probe", "x": 3, "y": 3, "w": 1, "h": 5, "ueBlueprint": "/Game/Instruments/Bioreactors/StirredTankBioreactor/BP_pH_Probe.BP_pH_Probe_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/ph_probe.png", "thumbnailImage": "devices/ph_probe.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Stirred_Tank_Bioreactor_-_dO2_Sensor_0", "name": "Stirred Tank Bioreactor - dO2 Sensor", "x": 4, "y": 3, "w": 1, "h": 4, "ueBlueprint": "/Game/Instruments/Bioreactors/StirredTankBioreactor/BP_d02_Sensor.BP_d02_Sensor_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/o2_sensor.png", "thumbnailImage": "devices/o2_sensor.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Stirred_Tank_Bioreactor_-_Antifoam_Sensor_0", "name": "Stirred Tank Bioreactor - Antifoam Sensor", "x": 5, "y": 3, "w": 1, "h": 4, "ueBlueprint": "/Game/Instruments/Bioreactors/StirredTankBioreactor/BP_Antifoam_Sensor.BP_Antifoam_Sensor_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/antifoam_sensor.png", "thumbnailImage": "devices/antifoam_sensor.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Stirred_Tank_Bioreactor_-_Vessel_0", "name": "Stirred Tank Bioreactor - Vessel", "x": 9, "y": 3, "w": 6, "h": 5, "ueBlueprint": "/Game/Instruments/Bioreactors/StirredTankBioreactor/BP_Stirred_Tank_Bioreactor.BP_Stirred_Tank_Bioreactor_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/univessel.png", "thumbnailImage": "devices/univessel.png", "isSurface": false, "requiresSurface": true, "items": [], "parts": [ { "slot": "TemperatureProbeSnap", "ueBlueprint": "/Game/Instruments/Bioreactors/StirredTankBioreactor/BP_Cap_Bioreactor_Probe_Insert.BP_Cap_Bioreactor_Probe_Insert_C", "parts": [] }, { "slot": "PHProbeSnap", "ueBlueprint": "/Game/Instruments/Bioreactors/StirredTankBioreactor/BP_Cap_Bioreactor_Probe_Insert.BP_Cap_Bioreactor_Probe_Insert_C", "parts": [] }, { "slot": "OxygenSnap", "ueBlueprint": "/Game/Instruments/Bioreactors/StirredTankBioreactor/BP_Cap_Bioreactor_Probe_Insert.BP_Cap_Bioreactor_Probe_Insert_C", "parts": [] }, { "slot": "AntifoamSnap", "ueBlueprint": "/Game/Instruments/Bioreactors/StirredTankBioreactor/BP_Cap_Bioreactor_Probe_Insert.BP_Cap_Bioreactor_Probe_Insert_C", "parts": [] } ] } ] }, { "id": "Bio_Bin_6L_0", "name": "Bio Bin 6L", "x": 25, "y": 13, "w": 5, "h": 4, "ueBlueprint": "/Game/Instruments/Bins/BP_Bin_6L_Bio.BP_Bin_6L_Bio_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "cleaning/bio_bin_6l.png", "thumbnailImage": "cleaning/bio_bin_6l.png", "isSurface": false, "requiresSurface": true, "items": [] } ], "thumbnailImage": "", "gridImage": "", "parts": [] } }, { "name": "Tube welder SOP", "steps": [ "Purpose and scope: This document describes the general operation of a Tube Welder under sterile conditions.", "Turn on Microbiological safety cabinet (MSC) by pressing the power button on the MSC’s touch display. ", "On the MSC’s touch display, turn on MSC light (lightbulb button).", "On the MSC’s touch display, turn on laminar airflow (fan).", "On the MSC’s touch display, use the upwards arrow button to lift MSC glass sash. Wait until the sash is fully open before continuing.", "Grab the ethanol spray bottle located on the table opposite the MSC and liberally spray both hands to sanitise them before working in the MSC.", "Press tick to enter on the user details on the tube welder screen.", "Open the tube welder lid.", "Remove the tube holders from inside the tube welder.", "Close the lid of the tube welder.", "On the display screen you will see ‘Initialize Axis’ screen with a time bar. Wait for this to complete.", "Once the lid has automatically reopened, insert the two tube holders back into the tube welder.", "Retrieve a cutting blade from the blade box and place the cutting blade in the blade slot inside the tube welder above the tube holders.", "Remove a sterile tube from the Tubing dispenser.", "Attach a filter or connector from the dispensers at each end of the sterile tubing.", "Repeat this for a second set of sterile tubing.", "Place the centre of 2 sterile tubes horizontal into the tube welder holders in the device.", "Close the tube welder lid.", "Press the 'tick' button on the weld screen to begin welding.", "Once the time bar has finished, the lid should open on completion of the process.", "Remove the blade from the tube welder and place in the sharps bin.", "Remove the welded tubing from the device.", "Close the lid." ], "layout": { "id": "main", "name": "Main grid", "x": 0, "y": 0, "w": 40, "h": 40, "ueBlueprint": "", "relativeRotation": 0, "absoluteRotation": 0, "isSurface": true, "requiresSurface": false, "items": [ { "id": "MSC_Large_0", "name": "MSC Large", "x": 1, "y": 0, "w": 36, "h": 17, "ueBlueprint": "/Game/Instruments/MSC/BP_MSC_Herasafe_Standing_Large.BP_MSC_Herasafe_Standing_Large_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/msc_large.png", "thumbnailImage": "devices/msc_large.png", "isSurface": true, "requiresSurface": false, "items": [ { "id": "Tube_Welder_Aseptic_0", "name": "Tube Welder Aseptic", "x": 9, "y": 1, "w": 6, "h": 11, "ueBlueprint": "/Game/Instruments/TubeWelder/BP_Tube_Welder.BP_Tube_Welder_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/tube_welder_aseptic_sartorius.png", "thumbnailImage": "devices/tube_welder_aseptic_sartorius.png", "isSurface": false, "requiresSurface": true, "parts": [ { "slot": "TubeHolderSnap1", "ueBlueprint": "/Game/Instruments/TubeWelder/BP_Welder_Tube_Holder.BP_Welder_Tube_Holder_C", "parts": [] }, { "slot": "TubeHolderSnap2", "ueBlueprint": "/Game/Instruments/TubeWelder/BP_Welder_Tube_Holder.BP_Welder_Tube_Holder_C", "parts": [] } ], "items": [] }, { "id": "Bin_Sharps_0", "name": "Bin Sharps", "x": 2, "y": 2, "w": 4, "h": 4, "ueBlueprint": "/Game/Instruments/Bins/BP_Bin_Sharps.BP_Bin_Sharps_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "cleaning/bin_sharps.png", "thumbnailImage": "cleaning/bin_sharps.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Tube_Welder_Aseptic_-_Blades_0", "name": "Tube Welder Aseptic - Blades", "x": 17, "y": 2, "w": 2, "h": 1, "ueBlueprint": "/Game/Instruments/TubeWelder/BP_Dispenser_Welder_Blades.BP_Dispenser_Welder_Blades_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/tube_welder_blades.png", "thumbnailImage": "devices/tube_welder_blades.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Tubing_Generic_0", "name": "Tubing Generic", "x": 26, "y": 2, "w": 8, "h": 2, "ueBlueprint": "/Game/Instruments/Tubing/Dispensers/BP_Dispenser_Tubing.BP_Dispenser_Tubing_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "tubing/dispenser_tubing.png", "thumbnailImage": "tubing/tubing_generic.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Tube_Connector_Elbow_0", "name": "Tube Connector Elbow", "x": 31, "y": 5, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Tubing/Dispensers/BP_Dispenser_Connector_Elbow.BP_Dispenser_Connector_Elbow_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "tubing/dispenser_tube_elbow.png", "thumbnailImage": "tubing/tube_elbow.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Sterile_Filter_0", "name": "Sterile Filter", "x": 27, "y": 5, "w": 3, "h": 2, "ueBlueprint": "/Game/Instruments/Tubing/Dispensers/BP_Dispenser_Connector_Sterile_Filter.BP_Dispenser_Connector_Sterile_Filter_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "tubing/dispenser_sterile_filter.png", "thumbnailImage": "tubing/sterile_filter.png", "isSurface": false, "requiresSurface": true, "items": [] } ] }, { "id": "Bio_Bin_6L_0", "name": "Bio Bin 6L", "x": 27, "y": 25, "w": 4, "h": 5, "ueBlueprint": "/Game/Instruments/Bins/BP_Bin_6L_Bio.BP_Bin_6L_Bio_C", "absoluteRotation": 90, "relativeRotation": 90, "gridImage": "cleaning/bio_bin_6l.png", "thumbnailImage": "cleaning/bio_bin_6l.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Table_60_x_45_0", "name": "Table 60 x 45", "x": 31, "y": 21, "w": 9, "h": 12, "ueBlueprint": "/Game/Instruments/Tables/BP_Table_Stand_Small.BP_Table_Stand_Small_C", "absoluteRotation": 90, "relativeRotation": 90, "gridImage": "furniture/table_stand.png", "thumbnailImage": "furniture/table_stand.png", "isSurface": true, "requiresSurface": true, "items": [ { "id": "Ethanol_Spray_Bottle_0", "name": "Ethanol Spray Bottle", "x": 5, "y": 2, "w": 3, "h": 2, "ueBlueprint": "/Game/Instruments/Sanitising/BP_Spray.BP_Spray_C", "absoluteRotation": 0, "relativeRotation": 270, "gridImage": "cleaning/spray_bottle.png", "thumbnailImage": "cleaning/spray_bottle.png", "isSurface": false, "requiresSurface": true, "items": [] } ] } ], "thumbnailImage": "", "gridImage": "", "parts": [] } }, { "name": "PH meter calibration", "steps": [ "Purpose and scope: PH meter calibration is important as it ensures accurate pH measurements by adjusting the meter against standardized buffer solutions.", "This procedure should be done in a fume hood if possible. ", "Place Beaker underneath PH meter and turn on the PH meter.", "Remove protective moistened tip end cap and place on surface.", "Rinse electrode and PH meter in sterile distilled water into the beaker. ", "Press CAL (Calibrate button).", "Move water filled beaker to one side.", "Remove cap from PH 7 solution bottle and place below PH meter.", "Dip PH probe into sterile uncapped PH 7 solution + note down PH reading. ", " If it's reading is more than 0.05 pH away from the standard buffer solution, the PH meter will require re-calibration.", "Remove PH 7 solution from below probe.", "Immediately place beaker of distilled water below probe and rinse probe well with distilled water.", "Remove beaker from below probe and replace with uncapped PH 4 solution.", "Press CAL (Calibrate) and dip PH probe into sterile uncapped PH 4 solution + note down PH reading. ", "Remove PH 4 solution from below probe.", "Place beaker of distilled water below probe and rinse probe well with distilled water.", "Remove beaker from below probe and replace with uncapped PH 10 solution.", "Press CAL (Calibrate) and dip PH probe into sterile uncapped PH 10 solution + note down PH reading. ", "Remove PH 10 solution from below probe.", "Place beaker of distilled water below probe and rinse probe well with distilled water.", "Recap probe with protective moistened dip end cap. ", " Once the PH probe and meter is calibrated you can start measuring unknown solutions using the method below.", "Remove lid from solution of unknown PH.", "Grab at the serological pipette dispenser with one hand and grab serological pipette controller with the other hand.", "Attach serological pipette to controller by hovering it over the snapslot until it shows and then releasing.", "With your middle finger extended and your index finger pressed down on the withdraw button, withdraw liquid into the serological pipette controller whilst holding the pipette in the liquid. Remove enough to fill the bottom of the beaker. ", "Then with your index finger extended and middle finger down on the expel button, expel enough liquid to fill the bottom of the beaker.", "Remove used serological pipette from controller once all liquid has been expelled and dispose of into contaminated waste bin.", "Re-cap the solution of unknown PH and place the pipette controller on the surface.", "Place beaker of solution to be measured below PH probe on stand.", "Re-press CAL on PH meter to perform a measurement.", "Remove protective moistened dip end cap and place on surface.", "Dip PH probe into unknown solution in beaker + note down PH reading. Press enter.", "Remove PH probe from the solution.", "Move unknown solution to the side.", "Rinse electrode and PH meter in sterile distilled water into the distilled water beaker.", "Re-measure solution of unknown PH 3x in total for accuracy, rinsing with distilled water into water beaker between each measurement.", "Move unknown solution to the side and dispose of/store as intended.", "Rinse electrode and PH meter in sterile distilled water into the distilled water beaker a final time.", "Recap probe with protective moistened dip end cap. ", "Switch off PH meter." ], "layout": { "id": "main", "name": "Main grid", "x": 0, "y": 0, "w": 40, "h": 40, "ueBlueprint": "", "relativeRotation": 0, "absoluteRotation": 0, "isSurface": true, "requiresSurface": false, "items": [ { "id": "Bio_Bin_6L_0", "name": "Bio Bin 6L", "x": 24, "y": 15, "w": 5, "h": 4, "ueBlueprint": "/Game/Instruments/Bins/BP_Bin_6L_Bio.BP_Bin_6L_Bio_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "cleaning/bio_bin_6l.png", "thumbnailImage": "cleaning/bio_bin_6l.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Table_180_x_60_0", "name": "Table 180 x 60", "x": 1, "y": 2, "w": 36, "h": 12, "ueBlueprint": "/Game/Instruments/Tables/BP_Table_180x60.BP_Table_180x60_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "furniture/table_180x60.png", "thumbnailImage": "furniture/table_large.png", "isSurface": true, "requiresSurface": true, "items": [ { "id": "Water_Wash_Bottle_0", "name": "Water Wash Bottle", "x": 2, "y": 1, "w": 3, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/SqueezeBottles/BP_Squeeze_Bottle_Water.BP_Squeeze_Bottle_Water_C", "absoluteRotation": 180, "relativeRotation": 180, "gridImage": "containers/wash_bottle_water_5l.png", "thumbnailImage": "containers/wash_bottle_water_5l.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Beaker_250ml_0", "name": "Beaker 250ml", "x": 2, "y": 4, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Beakers/BP_Beaker_250ml.BP_Beaker_250ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/beaker_250ml.png", "thumbnailImage": "containers/beaker_250ml.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Beaker_250ml_1", "name": "Beaker 250ml", "x": 2, "y": 6, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Beakers/BP_Beaker_250ml.BP_Beaker_250ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/beaker_250ml.png", "thumbnailImage": "containers/beaker_250ml.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Beaker_250ml_2", "name": "Beaker 250ml", "x": 2, "y": 8, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Beakers/BP_Beaker_250ml.BP_Beaker_250ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/beaker_250ml.png", "thumbnailImage": "containers/beaker_250ml.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Ph_Meter_0", "name": "Ph Meter", "x": 7, "y": 3, "w": 7, "h": 5, "ueBlueprint": "/Game/Instruments/Measuring/pHReader/BP_pH_Reader.BP_pH_Reader_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "devices/ph_meter.png", "thumbnailImage": "devices/ph_meter.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Seropipette_50ml_0", "name": "Seropipette 50ml", "x": 17, "y": 2, "w": 8, "h": 2, "ueBlueprint": 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"containers/bottle01_1l.png", "thumbnailImage": "containers/bottle1l_distilled_water.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "Acetate_Buffer_100ml_0", "name": "Acetate Buffer 100ml", "x": 30, "y": 6, "w": 2, "h": 2, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_Acetate_Buffer_100ml.BP_Bottle_Acetate_Buffer_100ml_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle02_100ml.png", "thumbnailImage": "containers/bottle100ml_acetate_buffer.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": "pH7_solution_100ml_0", "name": "pH7 solution 100ml", "x": 31, "y": 9, "w": 1, "h": 1, "ueBlueprint": "/Game/Instruments/Containers/Solutions/BP_Bottle_pH_7_Solution.BP_Bottle_pH_7_Solution_C", "absoluteRotation": 0, "relativeRotation": 0, "gridImage": "containers/bottle03_100ml.png", "thumbnailImage": "containers/bottle100ml_ph7.png", "isSurface": false, "requiresSurface": true, "items": [] }, { "id": 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